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Article: Characterization of AFLP sequences from regions of maize B chromosome defined by 12 B-10L translocations

TitleCharacterization of AFLP sequences from regions of maize B chromosome defined by 12 B-10L translocations
Authors
KeywordsSpecies Index: Zea Mays
Issue Date2005
PublisherGenetics Society of America. The Journal's web site is located at http://www.genetics.org/contents-by-date.0.shtml
Citation
Genetics, 2005, v. 169 n. 1, p. 375-388 How to Cite?
AbstractMaize B chromosome sequences have been previously cloned by microdissection, and all are proven to be highly repetitive, to be homologous to the normal complement, and to show no similarity to any published gene other than mobile elements. In this study, we isolated sequences from defined B regions. The strategy involved identification and then mapping of AFLP-derived B fragments before cloning. Of 14 B AFLPs, 13 were mapped by 12 B-10L translocations: 3 around the centromeric knob region, 3 in the proximal euchromatic, 1 around the border of proximal euchromatic and distal heterochromatic, and 6 in the distal heterochromatic region of the B long arm. The AFLP fragments were cloned and sequenced. Analogous to the microdissected sequences, all sequences were repetitive, and all but two were highly homologous to the A chromosomes. FISH signals of all but three clones appeared in pachytene B as well as in somatic A and B chromosomes. None of these clones exhibits identity to any published gene. Six clones displayed homology to two centromeric BACs, four to sequences of chromosomes 3, 4, 7, and 10, four to retrotransposons, and three to no sequence deposited in GenBank. Furthermore, flanking regions of two highly B-specific clones were characterized, showing extension of a B-exclusive nature. The possibility of the presence of novel B repeat (s) is discussed.
Persistent Identifierhttp://hdl.handle.net/10722/91002
ISSN
2015 Impact Factor: 4.644
2015 SCImago Journal Rankings: 4.270
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPeng, S-Fen_HK
dc.contributor.authorLin, Y-Pen_HK
dc.contributor.authorLin, B-Yen_HK
dc.date.accessioned2010-09-17T10:11:34Z-
dc.date.available2010-09-17T10:11:34Z-
dc.date.issued2005en_HK
dc.identifier.citationGenetics, 2005, v. 169 n. 1, p. 375-388en_HK
dc.identifier.issn0016-6731en_HK
dc.identifier.urihttp://hdl.handle.net/10722/91002-
dc.description.abstractMaize B chromosome sequences have been previously cloned by microdissection, and all are proven to be highly repetitive, to be homologous to the normal complement, and to show no similarity to any published gene other than mobile elements. In this study, we isolated sequences from defined B regions. The strategy involved identification and then mapping of AFLP-derived B fragments before cloning. Of 14 B AFLPs, 13 were mapped by 12 B-10L translocations: 3 around the centromeric knob region, 3 in the proximal euchromatic, 1 around the border of proximal euchromatic and distal heterochromatic, and 6 in the distal heterochromatic region of the B long arm. The AFLP fragments were cloned and sequenced. Analogous to the microdissected sequences, all sequences were repetitive, and all but two were highly homologous to the A chromosomes. FISH signals of all but three clones appeared in pachytene B as well as in somatic A and B chromosomes. None of these clones exhibits identity to any published gene. Six clones displayed homology to two centromeric BACs, four to sequences of chromosomes 3, 4, 7, and 10, four to retrotransposons, and three to no sequence deposited in GenBank. Furthermore, flanking regions of two highly B-specific clones were characterized, showing extension of a B-exclusive nature. The possibility of the presence of novel B repeat (s) is discussed.en_HK
dc.languageengen_HK
dc.publisherGenetics Society of America. The Journal's web site is located at http://www.genetics.org/contents-by-date.0.shtmlen_HK
dc.relation.ispartofGeneticsen_HK
dc.subjectSpecies Index: Zea Maysen_HK
dc.titleCharacterization of AFLP sequences from regions of maize B chromosome defined by 12 B-10L translocationsen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1534/genetics.104.032417en_HK
dc.identifier.pmid15489531-
dc.identifier.pmcidPMC1448868-
dc.identifier.scopuseid_2-s2.0-13744256210en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-13744256210&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume169en_HK
dc.identifier.issue1en_HK
dc.identifier.spage375en_HK
dc.identifier.epage388en_HK
dc.identifier.isiWOS:000227039900031-

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