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Article: Cloning and characterization of maize B chromosome sequences derived from microdissection

TitleCloning and characterization of maize B chromosome sequences derived from microdissection
Authors
KeywordsSpecies Index: Embryophyta
Zea Mays
Issue Date2003
PublisherGenetics Society of America. The Journal's web site is located at http://www.genetics.org/contents-by-date.0.shtml
Citation
Genetics, 2003, v. 164 n. 1, p. 299-310 How to Cite?
AbstractIsolation of sequences from the maize B chromosome is always hampered by its high homology with the normal complements. In this study, this handicap was overcome by cloning the sequences from the pachytene B chromosomes dissected out of a slide by a micromanipulator followed by degenerate oligonucleotide-primed PCR. The isolated sequences were found to hybridize with genomic DNA in a B-dosage-dependent manner and with the pachytene B chromosome by fluorescence in situ hybridization (FISH), corroborating their B origin. A total of 19 B sequences were isolated, all of which are repetitive and, with one exception, are homologous to the A chromosome(s). Three sequences have strong homology to maize sequences that include two knob repeats and one zein gene (noncoding region), and 10 others are homologous to the noncoding region of Adh1, Bz1, Gag, Zein, and B centromere to a lesser degree. Six sequences have no homology to any gene. In addition to FISH, the B-specific sequence and a partially B-specific one were also mapped, by seven newly characterized TB-10L translocations, to a similar location on the central portion of the distal heterochromatic region, spreading over a region of about one-third of the B chromosome.
Persistent Identifierhttp://hdl.handle.net/10722/90975
ISSN
2015 Impact Factor: 4.644
2015 SCImago Journal Rankings: 4.270
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheng, Y-Men_HK
dc.contributor.authorLin, B-Yen_HK
dc.date.accessioned2010-09-17T10:11:11Z-
dc.date.available2010-09-17T10:11:11Z-
dc.date.issued2003en_HK
dc.identifier.citationGenetics, 2003, v. 164 n. 1, p. 299-310en_HK
dc.identifier.issn0016-6731en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90975-
dc.description.abstractIsolation of sequences from the maize B chromosome is always hampered by its high homology with the normal complements. In this study, this handicap was overcome by cloning the sequences from the pachytene B chromosomes dissected out of a slide by a micromanipulator followed by degenerate oligonucleotide-primed PCR. The isolated sequences were found to hybridize with genomic DNA in a B-dosage-dependent manner and with the pachytene B chromosome by fluorescence in situ hybridization (FISH), corroborating their B origin. A total of 19 B sequences were isolated, all of which are repetitive and, with one exception, are homologous to the A chromosome(s). Three sequences have strong homology to maize sequences that include two knob repeats and one zein gene (noncoding region), and 10 others are homologous to the noncoding region of Adh1, Bz1, Gag, Zein, and B centromere to a lesser degree. Six sequences have no homology to any gene. In addition to FISH, the B-specific sequence and a partially B-specific one were also mapped, by seven newly characterized TB-10L translocations, to a similar location on the central portion of the distal heterochromatic region, spreading over a region of about one-third of the B chromosome.en_HK
dc.languageengen_HK
dc.publisherGenetics Society of America. The Journal's web site is located at http://www.genetics.org/contents-by-date.0.shtmlen_HK
dc.relation.ispartofGeneticsen_HK
dc.subjectSpecies Index: Embryophytaen_HK
dc.subjectZea Maysen_HK
dc.titleCloning and characterization of maize B chromosome sequences derived from microdissectionen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid12750341-
dc.identifier.scopuseid_2-s2.0-0038192137en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038192137&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume164en_HK
dc.identifier.issue1en_HK
dc.identifier.spage299en_HK
dc.identifier.epage310en_HK
dc.identifier.isiWOS:000183119700027-

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