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Article: Different functional types of bipolar cells use different gap-junctional proteins

TitleDifferent functional types of bipolar cells use different gap-junctional proteins
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2005
PublisherSociety for Neuroscience. The Journal's web site is located at http://www.jneurosci.org
Citation
Journal Of Neuroscience, 2005, v. 25 n. 28, p. 6696-6701 How to Cite?
AbstractRod signals are transmitted to ON retinal ganglion cells by means of gap junctions between All amacrine cells and ON bipolars. The All amacrine cells are known to express connexin36 (Cx36), but previous studies of Cx36 in ON cone bipolars have been ambiguous. Here, we studied bipolar cells in a transgenic mouse line that expresses high levels of green fluorescent protein (GFP) in one type of ON cone bipolar cell. We found strong Cx36 immunostaining in the axon terminals of the GFP-labeled type 357 bipolar cells in both vertical sections and whole mounts of the retina. This finding was confirmed by single-cell immunostaining and single-cell reverse transcription-PCR (RT-PCR). As reported previously (Maxeiner et al., 2005), Cx45 was found in some ON bipolar cells, but RT-PCR showed Cx36 and not Cx45 to be expressed by the type 357 bipolar cells. Some of the remaining GFP-negative bipolar cells expressed Cx45 but not Cx36. It appears that different types of ON cone bipolar cells express different connexins at their gap junctions with All amacrine cells. Copyright © 2005 Society for Neuroscience.
Persistent Identifierhttp://hdl.handle.net/10722/90958
ISSN
2015 Impact Factor: 5.924
2015 SCImago Journal Rankings: 5.105
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLin, Ben_HK
dc.contributor.authorJakobs, TCen_HK
dc.contributor.authorMasland, RHen_HK
dc.date.accessioned2010-09-17T10:10:56Z-
dc.date.available2010-09-17T10:10:56Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Neuroscience, 2005, v. 25 n. 28, p. 6696-6701en_HK
dc.identifier.issn0270-6474en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90958-
dc.description.abstractRod signals are transmitted to ON retinal ganglion cells by means of gap junctions between All amacrine cells and ON bipolars. The All amacrine cells are known to express connexin36 (Cx36), but previous studies of Cx36 in ON cone bipolars have been ambiguous. Here, we studied bipolar cells in a transgenic mouse line that expresses high levels of green fluorescent protein (GFP) in one type of ON cone bipolar cell. We found strong Cx36 immunostaining in the axon terminals of the GFP-labeled type 357 bipolar cells in both vertical sections and whole mounts of the retina. This finding was confirmed by single-cell immunostaining and single-cell reverse transcription-PCR (RT-PCR). As reported previously (Maxeiner et al., 2005), Cx45 was found in some ON bipolar cells, but RT-PCR showed Cx36 and not Cx45 to be expressed by the type 357 bipolar cells. Some of the remaining GFP-negative bipolar cells expressed Cx45 but not Cx36. It appears that different types of ON cone bipolar cells express different connexins at their gap junctions with All amacrine cells. Copyright © 2005 Society for Neuroscience.en_HK
dc.languageengen_HK
dc.publisherSociety for Neuroscience. The Journal's web site is located at http://www.jneurosci.orgen_HK
dc.relation.ispartofJournal of Neuroscienceen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.subject.meshAmacrine Cells - physiologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Communicationen_HK
dc.subject.meshConnexins - analysis - biosynthesis - genetics - physiologyen_HK
dc.subject.meshFluorescent Dyes - analysisen_HK
dc.subject.meshGap Junctions - physiologyen_HK
dc.subject.meshGreen Fluorescent Proteins - analysis - geneticsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Transgenicen_HK
dc.subject.meshMicroinjectionsen_HK
dc.subject.meshMicroscopy, Confocalen_HK
dc.subject.meshNerve Tissue Proteins - analysis - biosynthesis - genetics - physiologyen_HK
dc.subject.meshRNA, Messenger - biosynthesis - geneticsen_HK
dc.subject.meshRetinal Bipolar Cells - physiologyen_HK
dc.subject.meshRetinal Cone Photoreceptor Cells - cytology - physiologyen_HK
dc.subject.meshRetinal Rod Photoreceptor Cells - cytology - physiologyen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.titleDifferent functional types of bipolar cells use different gap-junctional proteinsen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.identifier.authorityLin, B=rp01356en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1523/JNEUROSCI.1894-05.2005en_HK
dc.identifier.pmid16014731en_HK
dc.identifier.scopuseid_2-s2.0-22244479983en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-22244479983&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue28en_HK
dc.identifier.spage6696en_HK
dc.identifier.epage6701en_HK
dc.identifier.isiWOS:000230478100023-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLin, B=36165916900en_HK
dc.identifier.scopusauthoridJakobs, TC=6701452375en_HK
dc.identifier.scopusauthoridMasland, RH=7007167900en_HK

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