File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Remodeling of cone photoreceptor cells after rod degeneration in rd mice

TitleRemodeling of cone photoreceptor cells after rod degeneration in rd mice
Authors
Keywordscone photoreceptors
degeneration
rd1 mouse
remodeling
retina
viral vector
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexer
Citation
Experimental Eye Research, 2009, v. 88 n. 3, p. 589-599 How to Cite?
AbstractWe studied the survival of cone photoreceptors following the degeneration of rods in the rd mouse. Cones were visualized by selective expression of green fluorescent protein (GFP) following transduction with an adeno-associated virus (AAV) vector. As previously reported, many cones survive after the initial degeneration of the rods. Soon after the initial degeneration, they lose their outer segments and all but a vestigial inner segment; and they partially retract or lose their axon and synaptic pedicle. However, they retain many fundamental features of the cone phenotype, and for many weeks show a polarized morphology indicative of substantial regrowth of processes. The cells retain their laminar position, forming a cell row just distal to a much thinned outer plexiform layer. The somata subsequently enlarge. Most of the cells extend bipolar processes, recreating the original bipolar morphology of a photoreceptor cell - though now turned on its side relative to the native position. The cells express short- or middle-wavelength opsins, recoverin and connexin36. One or more of the polarized processes could often be shown to contain synaptic ribbons, as visualized by antibodies against RIBEYE. The cones do not express protein kinase C alpha, Go alpha, ChX10 or calbindin, markers of bipolar or horizontal cells. The partially differentiated cone morphology persists for at least several months, after which the processes begin to retract and there is slow loss of the cells. Thus, during the time following the loss of their rod-dominated microenvironment, the cones achieve a semi-stable state in which much of their normal phenotype is preserved. Cone photoreceptors in retinas of human RP donors appear from their morphology to undergo a similar progression. The therapeutic window for rescue of cone photoreceptors may be longer than would have been thought.
Persistent Identifierhttp://hdl.handle.net/10722/90957
ISSN
2015 Impact Factor: 2.998
2015 SCImago Journal Rankings: 1.218
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
NIHEY 017169
R01 EY12654
Italian CNR
Funding Information:

This work was supported by NIH grant EY 017169. RHM is a Senior Investigator of Research to Prevent Blindness. ES was supported by the Italian CNR and NIH grant R01 EY12654.

References

 

DC FieldValueLanguage
dc.contributor.authorLin, Ben_HK
dc.contributor.authorMasland, RHen_HK
dc.contributor.authorStrettoi, Een_HK
dc.date.accessioned2010-09-17T10:10:55Z-
dc.date.available2010-09-17T10:10:55Z-
dc.date.issued2009en_HK
dc.identifier.citationExperimental Eye Research, 2009, v. 88 n. 3, p. 589-599en_HK
dc.identifier.issn0014-4835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90957-
dc.description.abstractWe studied the survival of cone photoreceptors following the degeneration of rods in the rd mouse. Cones were visualized by selective expression of green fluorescent protein (GFP) following transduction with an adeno-associated virus (AAV) vector. As previously reported, many cones survive after the initial degeneration of the rods. Soon after the initial degeneration, they lose their outer segments and all but a vestigial inner segment; and they partially retract or lose their axon and synaptic pedicle. However, they retain many fundamental features of the cone phenotype, and for many weeks show a polarized morphology indicative of substantial regrowth of processes. The cells retain their laminar position, forming a cell row just distal to a much thinned outer plexiform layer. The somata subsequently enlarge. Most of the cells extend bipolar processes, recreating the original bipolar morphology of a photoreceptor cell - though now turned on its side relative to the native position. The cells express short- or middle-wavelength opsins, recoverin and connexin36. One or more of the polarized processes could often be shown to contain synaptic ribbons, as visualized by antibodies against RIBEYE. The cones do not express protein kinase C alpha, Go alpha, ChX10 or calbindin, markers of bipolar or horizontal cells. The partially differentiated cone morphology persists for at least several months, after which the processes begin to retract and there is slow loss of the cells. Thus, during the time following the loss of their rod-dominated microenvironment, the cones achieve a semi-stable state in which much of their normal phenotype is preserved. Cone photoreceptors in retinas of human RP donors appear from their morphology to undergo a similar progression. The therapeutic window for rescue of cone photoreceptors may be longer than would have been thought.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexeren_HK
dc.relation.ispartofExperimental Eye Researchen_HK
dc.subjectcone photoreceptorsen_HK
dc.subjectdegenerationen_HK
dc.subjectrd1 mouseen_HK
dc.subjectremodelingen_HK
dc.subjectretinaen_HK
dc.subjectviral vectoren_HK
dc.titleRemodeling of cone photoreceptor cells after rod degeneration in rd miceen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.identifier.authorityLin, B=rp01356en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.exer.2008.11.022en_HK
dc.identifier.pmid19087876-
dc.identifier.pmcidPMC2656412-
dc.identifier.scopuseid_2-s2.0-61549136093en_HK
dc.identifier.hkuros228173-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-61549136093&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume88en_HK
dc.identifier.issue3en_HK
dc.identifier.spage589en_HK
dc.identifier.epage599en_HK
dc.identifier.eissn1096-0007-
dc.identifier.isiWOS:000264783900032-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLin, B=36165916900en_HK
dc.identifier.scopusauthoridMasland, RH=7007167900en_HK
dc.identifier.scopusauthoridStrettoi, E=6701678655en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats