File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Relationships between Microbial Community Structure and Hydrochemistry in a Landfill Leachate-Polluted Aquifer

TitleRelationships between Microbial Community Structure and Hydrochemistry in a Landfill Leachate-Polluted Aquifer
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2001
PublisherAmerican Society for Microbiology
Citation
Applied and Environmental Microbiology, 2001, v. 67 n. 10, p. 4619-4629 How to Cite?
AbstractKnowledge about the relationship between microbial community structure and hydrogeochemistry (e.g., pollution, redox and degradation processes) in landfill leachate-polluted aquifers is required to develop tools for predicting and monitoring natural attenuation. In this study analyses of pollutant and redox chemistry were conducted in parallel with culture-independent profiling of microbial communities present in a well-defined aquifer (Banisveld, The Netherlands). Degradation of organic contaminants occurred under iron-reducing conditions in the plume of pollution, while upstream of the landfill and above the plume denitrification was the dominant redox process. Beneath the plume iron reduction occurred. Numerical comparison of 16S ribosomal DNA (rDNA)-based denaturing gradient gel electrophoresis (DGGE) profiles of Bacteria and Archaea in 29 groundwater samples revealed a clear difference between the microbial community structures inside and outside the contaminant plume. A similar relationship was not evident in sediment samples. DGGE data were supported by sequencing cloned 16S rDNA. Upstream of the landfill members of the β subclass of the class Proteobacteria (β-proteobacteria) dominated. This group was not encountered beneath the landfill, where gram-positive bacteria dominated. Further downstream the contribution of gram-positive bacteria to the clone library decreased, while the contribution of δ-proteobacteria strongly increased and β-proteobacteria reappeared. The β-proteobacteria (Acidovorax, Rhodoferax) differed considerably from those found upstream (Gallionella, Azoarcus). Direct comparisons of cloned 16S rDNA with bands in DGGE profiles revealed that the data from each analysis were comparable. A relationship was observed between the dominant redox processes and the bacteria identified. In the iron-reducing plume members of the family Geobacteraceae made a strong contribution to the microbial communities. Because the only known aromatic hydrocarbon-degrading, iron-reducing bacteria are Geobacter spp., their occurrence in landfill leachate-contaminated aquifers deserves more detailed consideration.
Persistent Identifierhttp://hdl.handle.net/10722/90918
ISSN
2015 Impact Factor: 3.823
2015 SCImago Journal Rankings: 1.891
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRöling, WFMen_HK
dc.contributor.authorVan Breukelen, BMen_HK
dc.contributor.authorBraster, Men_HK
dc.contributor.authorLin, Ben_HK
dc.contributor.authorVan Verseveld, HWen_HK
dc.date.accessioned2010-09-17T10:10:20Z-
dc.date.available2010-09-17T10:10:20Z-
dc.date.issued2001en_HK
dc.identifier.citationApplied and Environmental Microbiology, 2001, v. 67 n. 10, p. 4619-4629en_HK
dc.identifier.issn0099-2240en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90918-
dc.description.abstractKnowledge about the relationship between microbial community structure and hydrogeochemistry (e.g., pollution, redox and degradation processes) in landfill leachate-polluted aquifers is required to develop tools for predicting and monitoring natural attenuation. In this study analyses of pollutant and redox chemistry were conducted in parallel with culture-independent profiling of microbial communities present in a well-defined aquifer (Banisveld, The Netherlands). Degradation of organic contaminants occurred under iron-reducing conditions in the plume of pollution, while upstream of the landfill and above the plume denitrification was the dominant redox process. Beneath the plume iron reduction occurred. Numerical comparison of 16S ribosomal DNA (rDNA)-based denaturing gradient gel electrophoresis (DGGE) profiles of Bacteria and Archaea in 29 groundwater samples revealed a clear difference between the microbial community structures inside and outside the contaminant plume. A similar relationship was not evident in sediment samples. DGGE data were supported by sequencing cloned 16S rDNA. Upstream of the landfill members of the β subclass of the class Proteobacteria (β-proteobacteria) dominated. This group was not encountered beneath the landfill, where gram-positive bacteria dominated. Further downstream the contribution of gram-positive bacteria to the clone library decreased, while the contribution of δ-proteobacteria strongly increased and β-proteobacteria reappeared. The β-proteobacteria (Acidovorax, Rhodoferax) differed considerably from those found upstream (Gallionella, Azoarcus). Direct comparisons of cloned 16S rDNA with bands in DGGE profiles revealed that the data from each analysis were comparable. A relationship was observed between the dominant redox processes and the bacteria identified. In the iron-reducing plume members of the family Geobacteraceae made a strong contribution to the microbial communities. Because the only known aromatic hydrocarbon-degrading, iron-reducing bacteria are Geobacter spp., their occurrence in landfill leachate-contaminated aquifers deserves more detailed consideration.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiologyen_HK
dc.relation.ispartofApplied and Environmental Microbiologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleRelationships between Microbial Community Structure and Hydrochemistry in a Landfill Leachate-Polluted Aquiferen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/AEM.67.10.4619-4629.2001en_HK
dc.identifier.pmid11571165-
dc.identifier.pmcidPMC93212-
dc.identifier.scopuseid_2-s2.0-0035491310en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035491310&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume67en_HK
dc.identifier.issue10en_HK
dc.identifier.spage4619en_HK
dc.identifier.epage4629en_HK
dc.identifier.isiWOS:000171237700031-
dc.identifier.f10001000933-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats