File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Scopus: eid_2-s2.0-0027075937
- PMID: 1360994
- WOS: WOS:A1992KB98200002
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: IL-12 augments antigen-dependent proliferation of activated T lymphocytes
Title | IL-12 augments antigen-dependent proliferation of activated T lymphocytes |
---|---|
Authors | |
Keywords | Chemicals And Cas Registry Numbers |
Issue Date | 1992 |
Publisher | American Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org |
Citation | Journal of Immunology, 1992, v. 149 n. 12, p. 3778-3783 How to Cite? |
Abstract | Ag-dependent T cell activation requires multiple transmembrane signals including activation of Ag-specific T cell receptor in combination with signals delivered through cytokine receptors. IL-12 is a heterodimeric cytokine involved in the regulation of NK and T lymphocyte responses. In examining the role of IL-12 in T cell activation, we found a direct relationship between Ag stimulation and IL-12-induced proliferation. Unlike IL-2, which induced proliferation of CTL either in the presence or absence of a CD3/TCR co-signal, IL-12 mediated proliferation of CTL only when the cells were recently co-stimulated with alloantigen or solid-phase anti-CD3 antibody. After culture in the absence of alloantigen or anti-CD3 for 7 to 14 days, these CTL lost the ability to proliferate to IL-12 alone. Under these conditions, however, IL-12 synergized with low-dose IL-2 to induce CTL proliferation. Restimulation with alloantigen or solid-phase anti-CD3 restored the ability of the CTL to proliferate to IL-12 alone. Not all Ag signals resulted in IL-2 independent proliferation to IL-12. For example, CTL with specificity for influenza matrix peptide proliferated best when co- cultured with peptide Ag presented on self MHC and a combination of IL-2 and IL-12. This evidence suggests that IL-12 may be useful in expanding an Ag- specific T cell population, as the culture of CTL with IL-12 and low-dose IL- 2 leads to proliferation only in response to an Ag co-signal. |
Persistent Identifier | http://hdl.handle.net/10722/90913 |
ISSN | 2023 Impact Factor: 3.6 2023 SCImago Journal Rankings: 1.558 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bertagnolli, MM | en_HK |
dc.contributor.author | Lin, B-Y | en_HK |
dc.contributor.author | Young, D | en_HK |
dc.contributor.author | Herrmann, SH | en_HK |
dc.date.accessioned | 2010-09-17T10:10:16Z | - |
dc.date.available | 2010-09-17T10:10:16Z | - |
dc.date.issued | 1992 | en_HK |
dc.identifier.citation | Journal of Immunology, 1992, v. 149 n. 12, p. 3778-3783 | en_HK |
dc.identifier.issn | 0022-1767 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/90913 | - |
dc.description.abstract | Ag-dependent T cell activation requires multiple transmembrane signals including activation of Ag-specific T cell receptor in combination with signals delivered through cytokine receptors. IL-12 is a heterodimeric cytokine involved in the regulation of NK and T lymphocyte responses. In examining the role of IL-12 in T cell activation, we found a direct relationship between Ag stimulation and IL-12-induced proliferation. Unlike IL-2, which induced proliferation of CTL either in the presence or absence of a CD3/TCR co-signal, IL-12 mediated proliferation of CTL only when the cells were recently co-stimulated with alloantigen or solid-phase anti-CD3 antibody. After culture in the absence of alloantigen or anti-CD3 for 7 to 14 days, these CTL lost the ability to proliferate to IL-12 alone. Under these conditions, however, IL-12 synergized with low-dose IL-2 to induce CTL proliferation. Restimulation with alloantigen or solid-phase anti-CD3 restored the ability of the CTL to proliferate to IL-12 alone. Not all Ag signals resulted in IL-2 independent proliferation to IL-12. For example, CTL with specificity for influenza matrix peptide proliferated best when co- cultured with peptide Ag presented on self MHC and a combination of IL-2 and IL-12. This evidence suggests that IL-12 may be useful in expanding an Ag- specific T cell population, as the culture of CTL with IL-12 and low-dose IL- 2 leads to proliferation only in response to an Ag co-signal. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org | en_HK |
dc.relation.ispartof | Journal of Immunology | en_HK |
dc.subject | Chemicals And Cas Registry Numbers | en_HK |
dc.title | IL-12 augments antigen-dependent proliferation of activated T lymphocytes | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Lin, B:blin@hku.hk | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.pmid | 1360994 | - |
dc.identifier.scopus | eid_2-s2.0-0027075937 | en_HK |
dc.identifier.volume | 149 | en_HK |
dc.identifier.issue | 12 | en_HK |
dc.identifier.spage | 3778 | en_HK |
dc.identifier.epage | 3783 | en_HK |
dc.identifier.isi | WOS:A1992KB98200002 | - |
dc.identifier.issnl | 0022-1767 | - |