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Article: IL-12 augments antigen-dependent proliferation of activated T lymphocytes

TitleIL-12 augments antigen-dependent proliferation of activated T lymphocytes
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date1992
PublisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org
Citation
Journal of Immunology, 1992, v. 149 n. 12, p. 3778-3783 How to Cite?
AbstractAg-dependent T cell activation requires multiple transmembrane signals including activation of Ag-specific T cell receptor in combination with signals delivered through cytokine receptors. IL-12 is a heterodimeric cytokine involved in the regulation of NK and T lymphocyte responses. In examining the role of IL-12 in T cell activation, we found a direct relationship between Ag stimulation and IL-12-induced proliferation. Unlike IL-2, which induced proliferation of CTL either in the presence or absence of a CD3/TCR co-signal, IL-12 mediated proliferation of CTL only when the cells were recently co-stimulated with alloantigen or solid-phase anti-CD3 antibody. After culture in the absence of alloantigen or anti-CD3 for 7 to 14 days, these CTL lost the ability to proliferate to IL-12 alone. Under these conditions, however, IL-12 synergized with low-dose IL-2 to induce CTL proliferation. Restimulation with alloantigen or solid-phase anti-CD3 restored the ability of the CTL to proliferate to IL-12 alone. Not all Ag signals resulted in IL-2 independent proliferation to IL-12. For example, CTL with specificity for influenza matrix peptide proliferated best when co- cultured with peptide Ag presented on self MHC and a combination of IL-2 and IL-12. This evidence suggests that IL-12 may be useful in expanding an Ag- specific T cell population, as the culture of CTL with IL-12 and low-dose IL- 2 leads to proliferation only in response to an Ag co-signal.
Persistent Identifierhttp://hdl.handle.net/10722/90913
ISSN
2015 Impact Factor: 4.985
2015 SCImago Journal Rankings: 3.549
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBertagnolli, MMen_HK
dc.contributor.authorLin, B-Yen_HK
dc.contributor.authorYoung, Den_HK
dc.contributor.authorHerrmann, SHen_HK
dc.date.accessioned2010-09-17T10:10:16Z-
dc.date.available2010-09-17T10:10:16Z-
dc.date.issued1992en_HK
dc.identifier.citationJournal of Immunology, 1992, v. 149 n. 12, p. 3778-3783en_HK
dc.identifier.issn0022-1767en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90913-
dc.description.abstractAg-dependent T cell activation requires multiple transmembrane signals including activation of Ag-specific T cell receptor in combination with signals delivered through cytokine receptors. IL-12 is a heterodimeric cytokine involved in the regulation of NK and T lymphocyte responses. In examining the role of IL-12 in T cell activation, we found a direct relationship between Ag stimulation and IL-12-induced proliferation. Unlike IL-2, which induced proliferation of CTL either in the presence or absence of a CD3/TCR co-signal, IL-12 mediated proliferation of CTL only when the cells were recently co-stimulated with alloantigen or solid-phase anti-CD3 antibody. After culture in the absence of alloantigen or anti-CD3 for 7 to 14 days, these CTL lost the ability to proliferate to IL-12 alone. Under these conditions, however, IL-12 synergized with low-dose IL-2 to induce CTL proliferation. Restimulation with alloantigen or solid-phase anti-CD3 restored the ability of the CTL to proliferate to IL-12 alone. Not all Ag signals resulted in IL-2 independent proliferation to IL-12. For example, CTL with specificity for influenza matrix peptide proliferated best when co- cultured with peptide Ag presented on self MHC and a combination of IL-2 and IL-12. This evidence suggests that IL-12 may be useful in expanding an Ag- specific T cell population, as the culture of CTL with IL-12 and low-dose IL- 2 leads to proliferation only in response to an Ag co-signal.en_HK
dc.languageengen_HK
dc.publisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.orgen_HK
dc.relation.ispartofJournal of Immunologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleIL-12 augments antigen-dependent proliferation of activated T lymphocytesen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid1360994-
dc.identifier.scopuseid_2-s2.0-0027075937en_HK
dc.identifier.volume149en_HK
dc.identifier.issue12en_HK
dc.identifier.spage3778en_HK
dc.identifier.epage3783en_HK
dc.identifier.isiWOS:A1992KB98200002-

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