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Article: Crucial role of the 5′ conserved structure of Bamboo mosaic virus satellite RNA in downregulation of helper viral RNA replication

TitleCrucial role of the 5′ conserved structure of Bamboo mosaic virus satellite RNA in downregulation of helper viral RNA replication
Authors
KeywordsSpecies Index: Bamboo Mosaic Virus
Bamboo Mosaic Virus Satellite Rna
Miridae
Nicotiana Benthamiana
Issue Date2006
PublisherAmerican Society for Microbiology
Citation
Journal of Virology, 2006, v. 80 n. 5, p. 2566-2574 How to Cite?
AbstractSatellite RNA of Bamboo mosaic virus (satBaMV), a single-stranded mRNA type satellite encoding a protein of 20 kDa (P20), depends on the helper BaMV for replication and encapsidation. Two satBaMV isolates, BSF4 and BSL6, exhibit distinctly differential phenotypes in Nicotiana benthamiana plants when coinoculated with BaMV RNA. BSL6 significantly reduces BaMV RNA replication and suppresses the BaMV-induced symptoms, whereas BSF4 does not. By studies with chimeric satBaMVs generated by exchanging the components between BSF4 and BSL6, the genetic determinants responsible for the downregulation of BaMV replication and symptom expression were mapped at the 5′ untranslated region (UTR) of BSL6. The 5′ UTR of BSL6 alone is sufficient to diminish BaMV RNA replication when the 5′ UTR is inserted in cis into the BaMV expression vector or when coinoculation with mutants that block the synthesis of P20 protein takes place. Further, the 5′ UTR of natural satBaMV isolates contains one hypervariable (HV) region which folds into a conserved apical hairpin stem-loop (AHSL) structure (W. B. Yeh, Y. H. Hsu, II. C. Chen, and N. S. Lin, Virology 330:105-115, 2004). Interchanges of AHSL segment of HV regions between BSF4 and BSL6 led to the ability of chimeric satBaMV to interfere with BaMV replication and symptom expression. The conserved secondary structure within the HV region is a potent determinant of the downregulation of helper virus replication. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/90885
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHsu, Y-Hen_HK
dc.contributor.authorChen, H-Cen_HK
dc.contributor.authorCheng, Jen_HK
dc.contributor.authorAnnamali, Pen_HK
dc.contributor.authorLin, B-Yen_HK
dc.contributor.authorWu, C-Ten_HK
dc.contributor.authorYeh, W-Ben_HK
dc.contributor.authorLin, N-Sen_HK
dc.date.accessioned2010-09-17T10:09:50Z-
dc.date.available2010-09-17T10:09:50Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal of Virology, 2006, v. 80 n. 5, p. 2566-2574en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/90885-
dc.description.abstractSatellite RNA of Bamboo mosaic virus (satBaMV), a single-stranded mRNA type satellite encoding a protein of 20 kDa (P20), depends on the helper BaMV for replication and encapsidation. Two satBaMV isolates, BSF4 and BSL6, exhibit distinctly differential phenotypes in Nicotiana benthamiana plants when coinoculated with BaMV RNA. BSL6 significantly reduces BaMV RNA replication and suppresses the BaMV-induced symptoms, whereas BSF4 does not. By studies with chimeric satBaMVs generated by exchanging the components between BSF4 and BSL6, the genetic determinants responsible for the downregulation of BaMV replication and symptom expression were mapped at the 5′ untranslated region (UTR) of BSL6. The 5′ UTR of BSL6 alone is sufficient to diminish BaMV RNA replication when the 5′ UTR is inserted in cis into the BaMV expression vector or when coinoculation with mutants that block the synthesis of P20 protein takes place. Further, the 5′ UTR of natural satBaMV isolates contains one hypervariable (HV) region which folds into a conserved apical hairpin stem-loop (AHSL) structure (W. B. Yeh, Y. H. Hsu, II. C. Chen, and N. S. Lin, Virology 330:105-115, 2004). Interchanges of AHSL segment of HV regions between BSF4 and BSL6 led to the ability of chimeric satBaMV to interfere with BaMV replication and symptom expression. The conserved secondary structure within the HV region is a potent determinant of the downregulation of helper virus replication. Copyright © 2006, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiologyen_HK
dc.relation.ispartofJournal of Virologyen_HK
dc.subjectSpecies Index: Bamboo Mosaic Virusen_HK
dc.subjectBamboo Mosaic Virus Satellite Rnaen_HK
dc.subjectMiridaeen_HK
dc.subjectNicotiana Benthamianaen_HK
dc.titleCrucial role of the 5′ conserved structure of Bamboo mosaic virus satellite RNA in downregulation of helper viral RNA replicationen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/JVI.80.5.2566-2574.2006en_HK
dc.identifier.pmid16474162-
dc.identifier.pmcidPMC1395367-
dc.identifier.scopuseid_2-s2.0-33144479701en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33144479701&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume80en_HK
dc.identifier.issue5en_HK
dc.identifier.spage2566en_HK
dc.identifier.epage2574en_HK
dc.identifier.isiWOS:000235388400048-

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