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Article: Iron-reducing microorganisms in a landfill leachate-polluted aquifer: Complementing culture-independent information with enrichments and isolations

TitleIron-reducing microorganisms in a landfill leachate-polluted aquifer: Complementing culture-independent information with enrichments and isolations
Authors
KeywordsBioremediation
Competition
Geobacteraceae
Iron-Reduction
Landfill Leachate
Natural Attenuation
Issue Date2007
PublisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/01490451.asp
Citation
Geomicrobiology Journal, 2007, v. 24 n. 3-4, p. 283-294 How to Cite?
AbstractUsing culture-independent 16S rRNA gene-based methods, we previously observed that Geobacteraceae were a major component of the microbial communities in the iron-reducing aquifer polluted by the Banisveld landfill, The Netherlands. However, phylogenetic information does not tell about the functional potential of the detected Geobacteraceae, nor can phylogenetic information easily be used to establish the presence of other iron-reducers. Therefore, we enriched for iron-reducing consortia using a range of culturing media, with various electron donors and acceptors and varying incubation conditions (pH, temperature), and by applying dilution-to-extinction culturing. Enrichments and strains isolated from these enrichments were characterized by 16S rRNA gene-based methods. The number of culturable iron-reducers was less than 110 iron-reducing bacteria per gram of sediment. The Geobacter phylotype that was previously found to constitute a major part of the microbial communities in a part of the aquifer where organic matter was attenuated at a relatively high rate, was not isolated. The isolation of another Geobacter strain and Serratia, Clostridium, Rhodoferax and Desulfitobacteriumstrains suggest the presence of a diverse iron-reducing community. Physiological capabilities of the isolates are described and discussed in relation to the hydrogeochemistry and the high abundance of Geobacteraceae in the aquifer polluted by the Banisveld landfill.
Persistent Identifierhttp://hdl.handle.net/10722/90866
ISSN
2015 Impact Factor: 1.402
2015 SCImago Journal Rankings: 0.589
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLin, Ben_HK
dc.contributor.authorBraster, Men_HK
dc.contributor.authorRöling, WFMen_HK
dc.contributor.authorvan Breukelen, BMen_HK
dc.date.accessioned2010-09-17T10:09:33Z-
dc.date.available2010-09-17T10:09:33Z-
dc.date.issued2007en_HK
dc.identifier.citationGeomicrobiology Journal, 2007, v. 24 n. 3-4, p. 283-294en_HK
dc.identifier.issn0149-0451en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90866-
dc.description.abstractUsing culture-independent 16S rRNA gene-based methods, we previously observed that Geobacteraceae were a major component of the microbial communities in the iron-reducing aquifer polluted by the Banisveld landfill, The Netherlands. However, phylogenetic information does not tell about the functional potential of the detected Geobacteraceae, nor can phylogenetic information easily be used to establish the presence of other iron-reducers. Therefore, we enriched for iron-reducing consortia using a range of culturing media, with various electron donors and acceptors and varying incubation conditions (pH, temperature), and by applying dilution-to-extinction culturing. Enrichments and strains isolated from these enrichments were characterized by 16S rRNA gene-based methods. The number of culturable iron-reducers was less than 110 iron-reducing bacteria per gram of sediment. The Geobacter phylotype that was previously found to constitute a major part of the microbial communities in a part of the aquifer where organic matter was attenuated at a relatively high rate, was not isolated. The isolation of another Geobacter strain and Serratia, Clostridium, Rhodoferax and Desulfitobacteriumstrains suggest the presence of a diverse iron-reducing community. Physiological capabilities of the isolates are described and discussed in relation to the hydrogeochemistry and the high abundance of Geobacteraceae in the aquifer polluted by the Banisveld landfill.en_HK
dc.languageengen_HK
dc.publisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/01490451.aspen_HK
dc.relation.ispartofGeomicrobiology Journalen_HK
dc.subjectBioremediationen_HK
dc.subjectCompetitionen_HK
dc.subjectGeobacteraceaeen_HK
dc.subjectIron-Reductionen_HK
dc.subjectLandfill Leachateen_HK
dc.subjectNatural Attenuationen_HK
dc.titleIron-reducing microorganisms in a landfill leachate-polluted aquifer: Complementing culture-independent information with enrichments and isolationsen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/01490450701456834en_HK
dc.identifier.scopuseid_2-s2.0-34547494863en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34547494863&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue3-4en_HK
dc.identifier.spage283en_HK
dc.identifier.epage294en_HK
dc.identifier.eissn1521-0529-
dc.identifier.isiWOS:000248266400014-

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