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Article: RNF8 Transduces the DNA-Damage Signal via Histone Ubiquitylation and Checkpoint Protein Assembly

TitleRNF8 Transduces the DNA-Damage Signal via Histone Ubiquitylation and Checkpoint Protein Assembly
Authors
KeywordsDNA
PROTEINS
SIGNALING
Issue Date2007
PublisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cell
Citation
Cell, 2007, v. 131 n. 5, p. 901-914 How to Cite?
AbstractDNA-damage signaling utilizes a multitude of posttranslational modifiers as molecular switches to regulate cell-cycle checkpoints, DNA repair, cellular senescence, and apoptosis. Here we show that RNF8, a FHA/RING domain-containing protein, plays a critical role in the early DNA-damage response. We have solved the X-ray crystal structure of the FHA domain structure at 1.35 Å. We have shown that RNF8 facilitates the accumulation of checkpoint mediator proteins BRCA1 and 53BP1 to the damaged chromatin, on one hand through the phospho-dependent FHA domain-mediated binding of RNF8 to MDC1, on the other hand via its role in ubiquitylating H2AX and possibly other substrates at damage sites. Moreover, RNF8-depleted cells displayed a defective G2/M checkpoint and increased IR sensitivity. Together, our study implicates RNF8 as a novel DNA-damage-responsive protein that integrates protein phosphorylation and ubiquitylation signaling and plays a critical role in the cellular response to genotoxic stress. © 2007 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/90859
ISSN
2015 Impact Factor: 28.71
2015 SCImago Journal Rankings: 28.188
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuen, MSYen_HK
dc.contributor.authorGrant, Ren_HK
dc.contributor.authorManke, Ien_HK
dc.contributor.authorMinn, Ken_HK
dc.contributor.authorYu, Xen_HK
dc.contributor.authorYaffe, MBen_HK
dc.contributor.authorChen, Jen_HK
dc.date.accessioned2010-09-17T10:09:27Z-
dc.date.available2010-09-17T10:09:27Z-
dc.date.issued2007en_HK
dc.identifier.citationCell, 2007, v. 131 n. 5, p. 901-914en_HK
dc.identifier.issn0092-8674en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90859-
dc.description.abstractDNA-damage signaling utilizes a multitude of posttranslational modifiers as molecular switches to regulate cell-cycle checkpoints, DNA repair, cellular senescence, and apoptosis. Here we show that RNF8, a FHA/RING domain-containing protein, plays a critical role in the early DNA-damage response. We have solved the X-ray crystal structure of the FHA domain structure at 1.35 Å. We have shown that RNF8 facilitates the accumulation of checkpoint mediator proteins BRCA1 and 53BP1 to the damaged chromatin, on one hand through the phospho-dependent FHA domain-mediated binding of RNF8 to MDC1, on the other hand via its role in ubiquitylating H2AX and possibly other substrates at damage sites. Moreover, RNF8-depleted cells displayed a defective G2/M checkpoint and increased IR sensitivity. Together, our study implicates RNF8 as a novel DNA-damage-responsive protein that integrates protein phosphorylation and ubiquitylation signaling and plays a critical role in the cellular response to genotoxic stress. © 2007 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cellen_HK
dc.relation.ispartofCellen_HK
dc.subjectDNAen_HK
dc.subjectPROTEINSen_HK
dc.subjectSIGNALINGen_HK
dc.titleRNF8 Transduces the DNA-Damage Signal via Histone Ubiquitylation and Checkpoint Protein Assemblyen_HK
dc.typeArticleen_HK
dc.identifier.emailHuen, MSY:huen.michael@hku.hken_HK
dc.identifier.authorityHuen, MSY=rp01336en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.cell.2007.09.041en_HK
dc.identifier.pmid18001825-
dc.identifier.pmcidPMC2149842-
dc.identifier.scopuseid_2-s2.0-36249031962en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36249031962&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume131en_HK
dc.identifier.issue5en_HK
dc.identifier.spage901en_HK
dc.identifier.epage914en_HK
dc.identifier.isiWOS:000251800900013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.f10001098145-
dc.identifier.scopusauthoridHuen, MSY=23004751500en_HK
dc.identifier.scopusauthoridGrant, R=7402006067en_HK
dc.identifier.scopusauthoridManke, I=6601975451en_HK
dc.identifier.scopusauthoridMinn, K=6701859567en_HK
dc.identifier.scopusauthoridYu, X=35249962700en_HK
dc.identifier.scopusauthoridYaffe, MB=7101980618en_HK
dc.identifier.scopusauthoridChen, J=35261693300en_HK
dc.identifier.citeulike2105305-

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