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Article: Physical mapping of four RAPDs in the B chromosome of maize

TitlePhysical mapping of four RAPDs in the B chromosome of maize
Authors
KeywordsB Chromosome
B-A Translocation
Rapd
Zea Mays
Issue Date1997
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00122/index.htm
Citation
Theoretical and Applied Genetics, 1997, v. 94 n. 3-4, p. 534-538 How to Cite?
AbstractFour DNA fragments were amplified specifically from the B chromosome by PCR using random 10-base oligonucleotides as primers. The location of the fragments in the B chromosome was determined based on whether or not they were amplified from the hypoploid DNA generated by four B-A translocations, three of which break in the proximal euchromatic region and the fourth in the distal one-third of the heterochromatic region on the B long arm. Since the hypoploid DNA carries the portion of the B chromosome distal to the breakpoint of a translocation, the presence of a fragment in the hypoploid DNA, but not in the control (which is devoid of any B chromatin), indicates that the fragments is located in the B region distal to the breakpoint in the B long arm. Two fragments were mapped to the euchromatic region and two others to either the distal portion of the euchromatic region or the proximal two-thirds of the heterochromatic region. These fragments in turn mapped three B-A translocations whose breakpoints were located in the euchromatic region.
Persistent Identifierhttp://hdl.handle.net/10722/90847
ISSN
2015 Impact Factor: 3.9
2015 SCImago Journal Rankings: 1.930
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLin, B-Yen_HK
dc.contributor.authorChou, H-Pen_HK
dc.date.accessioned2010-09-17T10:09:16Z-
dc.date.available2010-09-17T10:09:16Z-
dc.date.issued1997en_HK
dc.identifier.citationTheoretical and Applied Genetics, 1997, v. 94 n. 3-4, p. 534-538en_HK
dc.identifier.issn0040-5752en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90847-
dc.description.abstractFour DNA fragments were amplified specifically from the B chromosome by PCR using random 10-base oligonucleotides as primers. The location of the fragments in the B chromosome was determined based on whether or not they were amplified from the hypoploid DNA generated by four B-A translocations, three of which break in the proximal euchromatic region and the fourth in the distal one-third of the heterochromatic region on the B long arm. Since the hypoploid DNA carries the portion of the B chromosome distal to the breakpoint of a translocation, the presence of a fragment in the hypoploid DNA, but not in the control (which is devoid of any B chromatin), indicates that the fragments is located in the B region distal to the breakpoint in the B long arm. Two fragments were mapped to the euchromatic region and two others to either the distal portion of the euchromatic region or the proximal two-thirds of the heterochromatic region. These fragments in turn mapped three B-A translocations whose breakpoints were located in the euchromatic region.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00122/index.htmen_HK
dc.relation.ispartofTheoretical and Applied Geneticsen_HK
dc.subjectB Chromosomeen_HK
dc.subjectB-A Translocationen_HK
dc.subjectRapden_HK
dc.subjectZea Maysen_HK
dc.titlePhysical mapping of four RAPDs in the B chromosome of maizeen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s001220050448en_HK
dc.identifier.scopuseid_2-s2.0-0030943281en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030943281&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume94en_HK
dc.identifier.issue3-4en_HK
dc.identifier.spage534en_HK
dc.identifier.epage538en_HK
dc.identifier.isiWOS:A1997WR39500035-

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