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Article: A defective RNA associated with bamboo mosaic virus and the possible common mechanisms for RNA recombination in potexviruses

TitleA defective RNA associated with bamboo mosaic virus and the possible common mechanisms for RNA recombination in potexviruses
Authors
KeywordsSpecies Index: Bamboo Mosaic Virus
Nicotiana Benthamiana
Potexvirus
Rna Viruses
Issue Date1999
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-8569
Citation
Virus Genes, 1999, v. 18 n. 2, p. 121-128 How to Cite?
AbstractA naturally occurring 1.1 kb RNA was isolated from purified virions of bamboo mosaic potexvirus isolate S (BaMV-S). This RNA is a defective RNA (D RNA) derived from a single internal deletion of the BaMV genome. A cDNA clone representing the complete nucleotide sequence of the BaMV-S D RNA was generated and its nucleotide sequence was determined. The BaMV D cDNA is 1015 nts in length [excluding the poly(A) tail] and consists of two regions corresponding to 867 nts of the 5' terminus and 148 nts of the 3' terminus of the BaMV genomic RNA. BaMV D cDNA contains a single open reading frame (ORF) encoding a putative 29.7 kDa protein comprised of a fusion of the first 258 amino acids of BaMV ORF 1 and the last 2 amino acids of coat protein. The coding capacity of D RNA was verified by in vitro translation of native BaMV-S D RNA and of 1.1 kb RNA transcribed in vitro from the full-length D cDNA. BaMV D RNA can be reproducibly generated by serial passages of BaMV-S in Nicotiana benthamiana and is the first D RNA in the potexvirus group shown to be generated de novo. Alignments of sequences surrounding the 5' and 3' junction borders of reported potexvirus D RNAs reveal a 65.2-84.6% sequence identity, suggesting that common mechanisms for viral RNA recombination are involved in the generation of potexvirus D RNAs.
Persistent Identifierhttp://hdl.handle.net/10722/90840
ISSN
2015 Impact Factor: 1.285
2015 SCImago Journal Rankings: 0.767
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYeh, T-Yen_HK
dc.contributor.authorLin, B-Yen_HK
dc.contributor.authorChang, Y-Cen_HK
dc.contributor.authorHsu, Y-Hen_HK
dc.contributor.authorLin, N-Sen_HK
dc.date.accessioned2010-09-17T10:09:10Z-
dc.date.available2010-09-17T10:09:10Z-
dc.date.issued1999en_HK
dc.identifier.citationVirus Genes, 1999, v. 18 n. 2, p. 121-128en_HK
dc.identifier.issn0920-8569en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90840-
dc.description.abstractA naturally occurring 1.1 kb RNA was isolated from purified virions of bamboo mosaic potexvirus isolate S (BaMV-S). This RNA is a defective RNA (D RNA) derived from a single internal deletion of the BaMV genome. A cDNA clone representing the complete nucleotide sequence of the BaMV-S D RNA was generated and its nucleotide sequence was determined. The BaMV D cDNA is 1015 nts in length [excluding the poly(A) tail] and consists of two regions corresponding to 867 nts of the 5' terminus and 148 nts of the 3' terminus of the BaMV genomic RNA. BaMV D cDNA contains a single open reading frame (ORF) encoding a putative 29.7 kDa protein comprised of a fusion of the first 258 amino acids of BaMV ORF 1 and the last 2 amino acids of coat protein. The coding capacity of D RNA was verified by in vitro translation of native BaMV-S D RNA and of 1.1 kb RNA transcribed in vitro from the full-length D cDNA. BaMV D RNA can be reproducibly generated by serial passages of BaMV-S in Nicotiana benthamiana and is the first D RNA in the potexvirus group shown to be generated de novo. Alignments of sequences surrounding the 5' and 3' junction borders of reported potexvirus D RNAs reveal a 65.2-84.6% sequence identity, suggesting that common mechanisms for viral RNA recombination are involved in the generation of potexvirus D RNAs.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-8569en_HK
dc.relation.ispartofVirus Genesen_HK
dc.subjectSpecies Index: Bamboo Mosaic Virusen_HK
dc.subjectNicotiana Benthamianaen_HK
dc.subjectPotexvirusen_HK
dc.subjectRna Virusesen_HK
dc.titleA defective RNA associated with bamboo mosaic virus and the possible common mechanisms for RNA recombination in potexvirusesen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1023/A:1008008400653en_HK
dc.identifier.pmid10403698-
dc.identifier.scopuseid_2-s2.0-0033048669en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033048669&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue2en_HK
dc.identifier.spage121en_HK
dc.identifier.epage128en_HK
dc.identifier.isiWOS:000080737100004-

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