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Article: Theta stimulation polymerizes actin in dendritic spines of hippocampus

TitleTheta stimulation polymerizes actin in dendritic spines of hippocampus
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2005
PublisherSociety for Neuroscience. The Journal's web site is located at http://www.jneurosci.org
Citation
Journal of Neuroscience, 2005, v. 25 n. 8, p. 2062-2069 How to Cite?
AbstractIt has been proposed that the endurance of long-term potentiation (LTP) depends on structural changes entailing reorganization of the spine actin cytoskeleton. The present study used a new technique involving intracellular and extracellular application of rhodamine-phalloidin to conventional hippocampal slices to test whether induction of LTP by naturalistic patterns of afferent activity selectively increases actin polymerization in juvenile to young adult spines. Rhodamine-phalloidin, which selectively binds to polymerized actin, was detected in perikarya and proximal dendrites of CA1 pyramidal cells that received low-frequency afferent activity but was essentially absent in spines and fine dendritic processes. Theta pattern stimulation induced LTP and caused a large (threefold), reliable increase in labeled spines and spine-like puncta in the proximal dendritic zone containing potentiated synapses. The spines frequently occurred in the absence of labeling to other structures but were also found in association with fluorescent dendritic processes. These effects were replicated (> 10-fold increase in labeled spines) using extracellular applications of rhodamine-phalloidin. Increases in labeling appeared within 2 min, were completely blocked by treatments that prevent LTP induction, and occurred in slices prepared from young adult rats. These results indicate that near-threshold conditions for inducing stable potentiation cause the rapid polymerization of actin in mature spines and suggest that the effect is both sufficiently discrete to satisfy the synapse-specificity rule of LTP as well as rapid enough to participate in the initial stages of LTP consolidation.
Persistent Identifierhttp://hdl.handle.net/10722/90820
ISSN
2015 Impact Factor: 5.924
2015 SCImago Journal Rankings: 5.105
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLin, Ben_HK
dc.contributor.authorKramár, EAen_HK
dc.contributor.authorBi, Xen_HK
dc.contributor.authorBrucher, FAen_HK
dc.contributor.authorGall, CMen_HK
dc.contributor.authorLynch, Gen_HK
dc.date.accessioned2010-09-17T10:08:52Z-
dc.date.available2010-09-17T10:08:52Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal of Neuroscience, 2005, v. 25 n. 8, p. 2062-2069en_HK
dc.identifier.issn0270-6474en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90820-
dc.description.abstractIt has been proposed that the endurance of long-term potentiation (LTP) depends on structural changes entailing reorganization of the spine actin cytoskeleton. The present study used a new technique involving intracellular and extracellular application of rhodamine-phalloidin to conventional hippocampal slices to test whether induction of LTP by naturalistic patterns of afferent activity selectively increases actin polymerization in juvenile to young adult spines. Rhodamine-phalloidin, which selectively binds to polymerized actin, was detected in perikarya and proximal dendrites of CA1 pyramidal cells that received low-frequency afferent activity but was essentially absent in spines and fine dendritic processes. Theta pattern stimulation induced LTP and caused a large (threefold), reliable increase in labeled spines and spine-like puncta in the proximal dendritic zone containing potentiated synapses. The spines frequently occurred in the absence of labeling to other structures but were also found in association with fluorescent dendritic processes. These effects were replicated (> 10-fold increase in labeled spines) using extracellular applications of rhodamine-phalloidin. Increases in labeling appeared within 2 min, were completely blocked by treatments that prevent LTP induction, and occurred in slices prepared from young adult rats. These results indicate that near-threshold conditions for inducing stable potentiation cause the rapid polymerization of actin in mature spines and suggest that the effect is both sufficiently discrete to satisfy the synapse-specificity rule of LTP as well as rapid enough to participate in the initial stages of LTP consolidation.en_HK
dc.languageengen_HK
dc.publisherSociety for Neuroscience. The Journal's web site is located at http://www.jneurosci.orgen_HK
dc.relation.ispartofJournal of Neuroscienceen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleTheta stimulation polymerizes actin in dendritic spines of hippocampusen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1523/JNEUROSCI.4283-04.2005en_HK
dc.identifier.pmid15728846-
dc.identifier.scopuseid_2-s2.0-14244254891en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-14244254891&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue8en_HK
dc.identifier.spage2062en_HK
dc.identifier.epage2069en_HK
dc.identifier.isiWOS:000227211000020-

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