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Article: Long-term potentiation alters the modulator pharmacology of AMPA-type glutamate receptors

TitleLong-term potentiation alters the modulator pharmacology of AMPA-type glutamate receptors
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2002
PublisherAmerican Physiological Society
Citation
Journal of Neurophysiology, 2002, v. 87 n. 6, p. 2790-2800 How to Cite?
AbstractChanges in the biophysical properties of AMPA-type glutamate receptors have been proposed to mediate the expression of long-term potentiation (LTP). The present study tested if, as predicted from this hypothesis, AMPA receptor modulators differentially affect potentiated versus control synaptic currents. Whole cell recordings were collected from CA1 pyramidal neurons in hippocampal slices from adult rats. Within-neuron comparisons were made of the excitatory postsynaptic currents (EPSCs) elicited by two separate groups of Schaffer-collateral/commissural synapses. LTP was induced by theta burst stimulation in one set of inputs; cyclothiazide (CTZ), a drug that acts on the desensitization kinetics of AMPA receptors, was infused 30 min later. The decay time constants of the potentiated EPSCs prior to drug infusion were slightly, but significantly, shorter than those of control EPSCs. CTZ slowed the decay of the EPSCs, as reported in prior studies, and did so to a significantly greater degree in the potentiated synapses. Additionally, infusion of CTZ resulted in significantly greater effects on amplitude in potentiated pathways as compared with control pathways. The interaction between LTP and CTZ was also obtained in a separate set of experiments in which GABA receptor antagonists were used to block inhibitory postsynaptic currents. Additionally, there was no significant change in paired-pulse facilitation in the presence of CTZ, indicating that presynaptic effects of the drug were negligible. These findings provide new evidence that LTP modifies AMPA receptor kinetics. Candidates for the changes responsible for the observed effects of LTP were evaluated using a model of AMPA receptor kinetics; a simple increase in the channel opening rate provided the most satisfactory match with the LTP data.
Persistent Identifierhttp://hdl.handle.net/10722/90789
ISSN
2015 Impact Factor: 2.653
2015 SCImago Journal Rankings: 2.230
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLin, Ben_HK
dc.contributor.authorBrücher, FAen_HK
dc.contributor.authorColgin, LLen_HK
dc.contributor.authorLynch, Gen_HK
dc.date.accessioned2010-09-17T10:08:25Z-
dc.date.available2010-09-17T10:08:25Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal of Neurophysiology, 2002, v. 87 n. 6, p. 2790-2800en_HK
dc.identifier.issn0022-3077en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90789-
dc.description.abstractChanges in the biophysical properties of AMPA-type glutamate receptors have been proposed to mediate the expression of long-term potentiation (LTP). The present study tested if, as predicted from this hypothesis, AMPA receptor modulators differentially affect potentiated versus control synaptic currents. Whole cell recordings were collected from CA1 pyramidal neurons in hippocampal slices from adult rats. Within-neuron comparisons were made of the excitatory postsynaptic currents (EPSCs) elicited by two separate groups of Schaffer-collateral/commissural synapses. LTP was induced by theta burst stimulation in one set of inputs; cyclothiazide (CTZ), a drug that acts on the desensitization kinetics of AMPA receptors, was infused 30 min later. The decay time constants of the potentiated EPSCs prior to drug infusion were slightly, but significantly, shorter than those of control EPSCs. CTZ slowed the decay of the EPSCs, as reported in prior studies, and did so to a significantly greater degree in the potentiated synapses. Additionally, infusion of CTZ resulted in significantly greater effects on amplitude in potentiated pathways as compared with control pathways. The interaction between LTP and CTZ was also obtained in a separate set of experiments in which GABA receptor antagonists were used to block inhibitory postsynaptic currents. Additionally, there was no significant change in paired-pulse facilitation in the presence of CTZ, indicating that presynaptic effects of the drug were negligible. These findings provide new evidence that LTP modifies AMPA receptor kinetics. Candidates for the changes responsible for the observed effects of LTP were evaluated using a model of AMPA receptor kinetics; a simple increase in the channel opening rate provided the most satisfactory match with the LTP data.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Societyen_HK
dc.relation.ispartofJournal of Neurophysiologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleLong-term potentiation alters the modulator pharmacology of AMPA-type glutamate receptorsen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid12037181-
dc.identifier.scopuseid_2-s2.0-0036083122en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036083122&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume87en_HK
dc.identifier.issue6en_HK
dc.identifier.spage2790en_HK
dc.identifier.epage2800en_HK
dc.identifier.isiWOS:000175878900016-

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