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Article: Glutamate receptors at bipolar synapses in the inner plexiform layer of primate retina: Light microscopic analysis

TitleGlutamate receptors at bipolar synapses in the inner plexiform layer of primate retina: Light microscopic analysis
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2003
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248
Citation
Journal Of Comparative Neurology, 2003, v. 466 n. 1, p. 136-147 How to Cite?
AbstractAt least 10 different types of bipolar cells have been distinguished in the primate retina. The axon terminals of these cells stratify in distinct strata in the inner plexiform layer and are involved in parallel pathways to distinct types of ganglion cells. Ionotropic glutamate receptor (GluR) subunits also show a stratified distribution in the inner plexiform layer. Here, we investigated whether different types of bipolar cells are associated with different types of ionotropic glutamate receptors in the inner retina of a New World primate, the common marmoset Callithrix jacchus. Vertical cryostat sections through central retina were double labeled with immunohistochemical markers for bipolar cell types and with antibodies to α -amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunits GluR1 to 4, kainate receptor subunits GluR6/7, and the NR1C2′ subunit of the N-methyl-D-aspartate (NMDA) receptor. The axon terminals of bipolar cell types were reconstructed from confocal sections, and the colocalized immunoreactive puncta were quantified. For all bipolar cell types, immunoreactive puncta for the AMPA receptor subunits GluR2, 2/3, and 4 were colocalized at highest densities, whereas GluR1-immunoreactive puncta were expressed at very low densities. The kainate receptor subunits GluR6/7 were predominantly associated with diffuse bipolar (DB6) and rod bipolar cells. The NMDA receptor subunit NR1C2′ was specifically colocalized with flat midget and DB3 axons. These findings suggest that rod and cone bipolar cell types contribute to multiple but distinct glutamate receptor pathways in primate retina. © 2003 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/90788
ISSN
2015 Impact Factor: 3.331
2015 SCImago Journal Rankings: 2.345
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGrünert, Uen_HK
dc.contributor.authorLin, Ben_HK
dc.contributor.authorMartin, PRen_HK
dc.date.accessioned2010-09-17T10:08:24Z-
dc.date.available2010-09-17T10:08:24Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Comparative Neurology, 2003, v. 466 n. 1, p. 136-147en_HK
dc.identifier.issn0021-9967en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90788-
dc.description.abstractAt least 10 different types of bipolar cells have been distinguished in the primate retina. The axon terminals of these cells stratify in distinct strata in the inner plexiform layer and are involved in parallel pathways to distinct types of ganglion cells. Ionotropic glutamate receptor (GluR) subunits also show a stratified distribution in the inner plexiform layer. Here, we investigated whether different types of bipolar cells are associated with different types of ionotropic glutamate receptors in the inner retina of a New World primate, the common marmoset Callithrix jacchus. Vertical cryostat sections through central retina were double labeled with immunohistochemical markers for bipolar cell types and with antibodies to α -amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunits GluR1 to 4, kainate receptor subunits GluR6/7, and the NR1C2′ subunit of the N-methyl-D-aspartate (NMDA) receptor. The axon terminals of bipolar cell types were reconstructed from confocal sections, and the colocalized immunoreactive puncta were quantified. For all bipolar cell types, immunoreactive puncta for the AMPA receptor subunits GluR2, 2/3, and 4 were colocalized at highest densities, whereas GluR1-immunoreactive puncta were expressed at very low densities. The kainate receptor subunits GluR6/7 were predominantly associated with diffuse bipolar (DB6) and rod bipolar cells. The NMDA receptor subunit NR1C2′ was specifically colocalized with flat midget and DB3 axons. These findings suggest that rod and cone bipolar cell types contribute to multiple but distinct glutamate receptor pathways in primate retina. © 2003 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248en_HK
dc.relation.ispartofJournal of Comparative Neurologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCallithrix - anatomy & histology - metabolismen_HK
dc.subject.meshFluorescent Antibody Techniqueen_HK
dc.subject.meshGlutamic Acid - metabolismen_HK
dc.subject.meshInterneurons - cytology - metabolismen_HK
dc.subject.meshMicroscopy, Confocalen_HK
dc.subject.meshPresynaptic Terminals - metabolism - ultrastructureen_HK
dc.subject.meshProtein Subunitsen_HK
dc.subject.meshReceptors, AMPA - metabolismen_HK
dc.subject.meshReceptors, Glutamate - metabolismen_HK
dc.subject.meshReceptors, Kainic Acid - metabolismen_HK
dc.subject.meshReceptors, N-Methyl-D-Aspartate - metabolismen_HK
dc.subject.meshRetina - cytology - metabolismen_HK
dc.subject.meshSynapses - metabolism - ultrastructureen_HK
dc.subject.meshSynaptic Transmission - physiologyen_HK
dc.subject.meshVision, Ocular - physiologyen_HK
dc.titleGlutamate receptors at bipolar synapses in the inner plexiform layer of primate retina: Light microscopic analysisen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.identifier.authorityLin, B=rp01356en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/cne.10862en_HK
dc.identifier.pmid14515245en_HK
dc.identifier.scopuseid_2-s2.0-0141705247en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0141705247&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume466en_HK
dc.identifier.issue1en_HK
dc.identifier.spage136en_HK
dc.identifier.epage147en_HK
dc.identifier.isiWOS:000185627800009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGrünert, U=7004613233en_HK
dc.identifier.scopusauthoridLin, B=36165916900en_HK
dc.identifier.scopusauthoridMartin, PR=7406040488en_HK

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