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Article: Phylogenetic and physiological diversity of dissimilatory ferric iron reducers in sediments of the polluted Scheldt estuary, Northwest Europe

TitlePhylogenetic and physiological diversity of dissimilatory ferric iron reducers in sediments of the polluted Scheldt estuary, Northwest Europe
Authors
KeywordsSpecies Index: Bacteria (Microorganisms)
Clostridium
Geobacteraceae
Ralstonia
Shewanella
Issue Date2007
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EMI
Citation
Environmental Microbiology, 2007, v. 9 n. 8, p. 1956-1968 How to Cite?
AbstractThe potential for dissimilatory ferric iron [Fe(III)] reduction in intertidal sediments of the polluted Scheldt estuary, Northwest Europe, was assessed by combining field-based geochemical measurements with laboratory experiments on the associated microbiology. Microbial communities at a freshwater and brackish location were characterized by culture-independent 16S rRNA gene analysis, as well as enrichments, strain isolation and physiological screening. Dilution-to-extinction batch enrichments using a variety of Fe(III) sources were performed. The dilution factor of the inoculum in the enrichments had a more determining effect on the Fe(III)-reducing microbial community structure than the Fe(III) source. Well-known Fe(III) reducers, including members of the family Geobacteraceae and the genus Shewanella, constituted only a small fraction (≤ 1%) of the in situ microbial community. Instead, facultative anaerobic Ralstonia and strictly anaerobic, spore-forming Clostridium species dominated Fe(III) reduction. These species were able to utilize a variety of electron acceptors. This flexibility may help the organisms to survive in the dynamic estuarine environment. The high diversity and abundance of culturable Fe(III) reducers (4.6 × 105 and 2.4 × 104 cells g-1 sediment at the freshwater and brackish site respectively), plus the high concentrations of chemically reducible solid-phase Fe(III) at the sites, implied a high potential for dissimilatory Fe(III) reduction in the estuarine sediments. Pore water chemical data further supported in situ dissimilatory Fe(III) reduction. © 2007 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/90775
ISSN
2015 Impact Factor: 5.932
2015 SCImago Journal Rankings: 3.002
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLin, Ben_HK
dc.contributor.authorHyacinthe, Cen_HK
dc.contributor.authorBonneville, Sen_HK
dc.contributor.authorBraster, Men_HK
dc.contributor.authorVan Cappellen, Pen_HK
dc.contributor.authorRöling, WFMen_HK
dc.date.accessioned2010-09-17T10:08:11Z-
dc.date.available2010-09-17T10:08:11Z-
dc.date.issued2007en_HK
dc.identifier.citationEnvironmental Microbiology, 2007, v. 9 n. 8, p. 1956-1968en_HK
dc.identifier.issn1462-2912en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90775-
dc.description.abstractThe potential for dissimilatory ferric iron [Fe(III)] reduction in intertidal sediments of the polluted Scheldt estuary, Northwest Europe, was assessed by combining field-based geochemical measurements with laboratory experiments on the associated microbiology. Microbial communities at a freshwater and brackish location were characterized by culture-independent 16S rRNA gene analysis, as well as enrichments, strain isolation and physiological screening. Dilution-to-extinction batch enrichments using a variety of Fe(III) sources were performed. The dilution factor of the inoculum in the enrichments had a more determining effect on the Fe(III)-reducing microbial community structure than the Fe(III) source. Well-known Fe(III) reducers, including members of the family Geobacteraceae and the genus Shewanella, constituted only a small fraction (≤ 1%) of the in situ microbial community. Instead, facultative anaerobic Ralstonia and strictly anaerobic, spore-forming Clostridium species dominated Fe(III) reduction. These species were able to utilize a variety of electron acceptors. This flexibility may help the organisms to survive in the dynamic estuarine environment. The high diversity and abundance of culturable Fe(III) reducers (4.6 × 105 and 2.4 × 104 cells g-1 sediment at the freshwater and brackish site respectively), plus the high concentrations of chemically reducible solid-phase Fe(III) at the sites, implied a high potential for dissimilatory Fe(III) reduction in the estuarine sediments. Pore water chemical data further supported in situ dissimilatory Fe(III) reduction. © 2007 The Authors.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EMIen_HK
dc.relation.ispartofEnvironmental Microbiologyen_HK
dc.subjectSpecies Index: Bacteria (Microorganisms)en_HK
dc.subjectClostridiumen_HK
dc.subjectGeobacteraceaeen_HK
dc.subjectRalstoniaen_HK
dc.subjectShewanellaen_HK
dc.titlePhylogenetic and physiological diversity of dissimilatory ferric iron reducers in sediments of the polluted Scheldt estuary, Northwest Europeen_HK
dc.typeArticleen_HK
dc.identifier.emailLin, B:blin@hku.hken_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1462-2920.2007.01312.xen_HK
dc.identifier.pmid17635542-
dc.identifier.scopuseid_2-s2.0-34447335642en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34447335642&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1956en_HK
dc.identifier.epage1968en_HK
dc.identifier.eissn1462-2920-
dc.identifier.isiWOS:000248451600008-
dc.identifier.citeulike1454622-

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