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Article: Loss of heterozygosity, by mitotic gene conversion and crossing over, causes strain-specific adenine mutants in constitutive diploid Candida albicans

TitleLoss of heterozygosity, by mitotic gene conversion and crossing over, causes strain-specific adenine mutants in constitutive diploid Candida albicans
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date1999
PublisherSociety for General Microbiology. The Journal's web site is located at http://mic.sgmjournals.org
Citation
Microbiology, 1999, v. 145 n. 7, p. 1623-1629 How to Cite?
AbstractMolecular evidence is provided in this paper to elucidate a long-standing intriguing phenomenon in fungal genetics: that many natural isolates of the constitutive diploid organism Candida albicans yield strain-specific, recessive mutants at a reproducible frequency that is as high as a few percent of the surviving cells after exposure to UV irradiation or other mutagens. Southern hybridization analysis and DNA sequence data indicated that C. albicans CA12, a clinical isolate, is heterozygous for the ADE2 gene, carrying one functional and one null allele. Sequence analysis of the null allele revealed the presence of a 1.3 kb deletion, which locates between two AATC repeats and spans the promoter and coding regions of the gene. The adenine auxotrophic mutants, which were readily isolated after UV irradiation of C. albicans CA12, were proved to be the segregants of mitotic recombination as they remained as diploid, not hemizygous or haploid, cells and were homozygous for ade2. Analysis of reciprocal products of the mitotic recombination detected that the process of loss of heterozygosity was mediated by mitotic crossing over (reciprocal exchange of genetic information) as well as gene conversion (non-reciprocal exchange of genetic information).
Persistent Identifierhttp://hdl.handle.net/10722/90727
ISSN
2015 Impact Factor: 2.268
2015 SCImago Journal Rankings: 1.144
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTsang, PWKen_HK
dc.contributor.authorCao, Ben_HK
dc.contributor.authorSiu, PYLen_HK
dc.contributor.authorWang, Jen_HK
dc.date.accessioned2010-09-17T10:07:24Z-
dc.date.available2010-09-17T10:07:24Z-
dc.date.issued1999en_HK
dc.identifier.citationMicrobiology, 1999, v. 145 n. 7, p. 1623-1629en_HK
dc.identifier.issn1350-0872en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90727-
dc.description.abstractMolecular evidence is provided in this paper to elucidate a long-standing intriguing phenomenon in fungal genetics: that many natural isolates of the constitutive diploid organism Candida albicans yield strain-specific, recessive mutants at a reproducible frequency that is as high as a few percent of the surviving cells after exposure to UV irradiation or other mutagens. Southern hybridization analysis and DNA sequence data indicated that C. albicans CA12, a clinical isolate, is heterozygous for the ADE2 gene, carrying one functional and one null allele. Sequence analysis of the null allele revealed the presence of a 1.3 kb deletion, which locates between two AATC repeats and spans the promoter and coding regions of the gene. The adenine auxotrophic mutants, which were readily isolated after UV irradiation of C. albicans CA12, were proved to be the segregants of mitotic recombination as they remained as diploid, not hemizygous or haploid, cells and were homozygous for ade2. Analysis of reciprocal products of the mitotic recombination detected that the process of loss of heterozygosity was mediated by mitotic crossing over (reciprocal exchange of genetic information) as well as gene conversion (non-reciprocal exchange of genetic information).en_HK
dc.languageengen_HK
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://mic.sgmjournals.orgen_HK
dc.relation.ispartofMicrobiologyen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.subject.meshAdenine - metabolismen_HK
dc.subject.meshBlotting, Southernen_HK
dc.subject.meshCandida albicans - genetics - metabolism - radiation effectsen_HK
dc.subject.meshCarboxy-Lyases - geneticsen_HK
dc.subject.meshChromosome Mappingen_HK
dc.subject.meshCrossing Over, Geneticen_HK
dc.subject.meshGene Conversionen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLoss of Heterozygosityen_HK
dc.subject.meshMitosisen_HK
dc.subject.meshMutagenesisen_HK
dc.subject.meshTransformation, Geneticen_HK
dc.subject.meshUltraviolet Raysen_HK
dc.titleLoss of heterozygosity, by mitotic gene conversion and crossing over, causes strain-specific adenine mutants in constitutive diploid Candida albicansen_HK
dc.typeArticleen_HK
dc.identifier.emailTsang, PWK:pwktsang@hku.hken_HK
dc.identifier.authorityTsang, PWK=rp01388en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10439401-
dc.identifier.scopuseid_2-s2.0-0032773629en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032773629&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume145en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1623en_HK
dc.identifier.epage1629en_HK
dc.identifier.isiWOS:000081550200012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTsang, PWK=8334953500en_HK
dc.identifier.scopusauthoridCao, B=16749359000en_HK
dc.identifier.scopusauthoridSiu, PYL=7004170707en_HK
dc.identifier.scopusauthoridWang, J=9637013000en_HK

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