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Article: Characterization of Actinobacillus actinomycetemcomitans isolated from young Chinese aggressive periodontitis patients

TitleCharacterization of Actinobacillus actinomycetemcomitans isolated from young Chinese aggressive periodontitis patients
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2005
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal Of Periodontal Research, 2005, v. 40 n. 3, p. 258-268 How to Cite?
AbstractObjective: This study characterized Actinobacillus actinomycetemcomitans isolates from young Chinese aggressive periodontitis patients. Methods: Subgingival plaque samples (two/subject) were collected from diseased subjects < 25 years old (n = 9, mean age 21.1 ± 1.6 years) and age-matched periodontitis-free controls (n = 47, mean age 22.0 ± 1.1 years). Selective and anaerobic culture were used. The serotype, leukotoxin gene (ltx) operon promoter and the cytolethal distending toxin (cdt) genes complex of the A. actinomycetemcomitans isolates were investigated. Effects of the isolates on non-keratinizing periodontal ligament epithelial cells monolayer were studied. Results: Diseased subjects had significantly higher full-mouth bleeding score (p = 0.002) and total viable counts from plaque samples (7.2 × 10 6 vs. 2.1 × 105 CFU/paperpoint, p < 0.005). A. actinomycetemcomitans was isolated from 67%/56% or 6%/4% of diseased or controls subject/sites, respectively (p < 0.001). The proportion of A. actinomycetemcomitans isolatable from aggressive periodontitis or periodontitis-free associated subgingival plaque was low (0.7% vs. 0.1%, p < 0.02). The serotype of the isolates was characterized. All isolates possessed 652-like ltx gene promoter and all but one serotype c isolate from a diseased patient had intact cdtABC genes. That particular strain appeared to confer the least cellular damages on periodontal ligament epithelial monolayer compared to others. Conclusion: This preliminary study confirmed the notion of increased prevalence and quantity of A. actinomycetemcomitans associated with aggressive periodontitis in young patients. The overall ltx promoter and cdt characteristics of the A. actinomycetemcomitans isolates, however, were similar among the diseased and control groups. A strain lacking the cdtABC gene appeared to be less damaging to a periodontal ligament epithelial cell model. Further studies therefore are warranted to clarify the pathogenic role and potentials of A. actinomycetemcomitans in aggressive periodontitis. © Blackwell Munksgaard 2005.
Persistent Identifierhttp://hdl.handle.net/10722/90726
ISSN
2015 Impact Factor: 2.474
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, WKen_HK
dc.contributor.authorNgai, VKSen_HK
dc.contributor.authorYau, JYYen_HK
dc.contributor.authorCheung, BPKen_HK
dc.contributor.authorTsang, PWKen_HK
dc.contributor.authorCorbet, EFen_HK
dc.date.accessioned2010-09-17T10:07:23Z-
dc.date.available2010-09-17T10:07:23Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Periodontal Research, 2005, v. 40 n. 3, p. 258-268en_HK
dc.identifier.issn0022-3484en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90726-
dc.description.abstractObjective: This study characterized Actinobacillus actinomycetemcomitans isolates from young Chinese aggressive periodontitis patients. Methods: Subgingival plaque samples (two/subject) were collected from diseased subjects < 25 years old (n = 9, mean age 21.1 ± 1.6 years) and age-matched periodontitis-free controls (n = 47, mean age 22.0 ± 1.1 years). Selective and anaerobic culture were used. The serotype, leukotoxin gene (ltx) operon promoter and the cytolethal distending toxin (cdt) genes complex of the A. actinomycetemcomitans isolates were investigated. Effects of the isolates on non-keratinizing periodontal ligament epithelial cells monolayer were studied. Results: Diseased subjects had significantly higher full-mouth bleeding score (p = 0.002) and total viable counts from plaque samples (7.2 × 10 6 vs. 2.1 × 105 CFU/paperpoint, p < 0.005). A. actinomycetemcomitans was isolated from 67%/56% or 6%/4% of diseased or controls subject/sites, respectively (p < 0.001). The proportion of A. actinomycetemcomitans isolatable from aggressive periodontitis or periodontitis-free associated subgingival plaque was low (0.7% vs. 0.1%, p < 0.02). The serotype of the isolates was characterized. All isolates possessed 652-like ltx gene promoter and all but one serotype c isolate from a diseased patient had intact cdtABC genes. That particular strain appeared to confer the least cellular damages on periodontal ligament epithelial monolayer compared to others. Conclusion: This preliminary study confirmed the notion of increased prevalence and quantity of A. actinomycetemcomitans associated with aggressive periodontitis in young patients. The overall ltx promoter and cdt characteristics of the A. actinomycetemcomitans isolates, however, were similar among the diseased and control groups. A strain lacking the cdtABC gene appeared to be less damaging to a periodontal ligament epithelial cell model. Further studies therefore are warranted to clarify the pathogenic role and potentials of A. actinomycetemcomitans in aggressive periodontitis. © Blackwell Munksgaard 2005.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1en_HK
dc.relation.ispartofJournal of Periodontal Researchen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.subject.meshActinobacillus Infectionsen_HK
dc.subject.meshActinobacillus actinomycetemcomitans - classification - genetics - isolation & purificationen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBacterial Toxins - analysisen_HK
dc.subject.meshCase-Control Studiesen_HK
dc.subject.meshExotoxins - analysisen_HK
dc.subject.meshHumansen_HK
dc.subject.meshPeriodontitis - microbiologyen_HK
dc.subject.meshSwineen_HK
dc.titleCharacterization of Actinobacillus actinomycetemcomitans isolated from young Chinese aggressive periodontitis patientsen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, WK:ewkleung@hkucc.hku.hken_HK
dc.identifier.emailTsang, PWK:pwktsang@hku.hken_HK
dc.identifier.emailCorbet, EF:efcorbet@hku.hken_HK
dc.identifier.authorityLeung, WK=rp00019en_HK
dc.identifier.authorityTsang, PWK=rp01388en_HK
dc.identifier.authorityCorbet, EF=rp00005en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1111/j.1600-0765.2005.00805.xen_HK
dc.identifier.pmid15853973-
dc.identifier.scopuseid_2-s2.0-18544387270en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18544387270&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue3en_HK
dc.identifier.spage258en_HK
dc.identifier.epage268en_HK
dc.identifier.isiWOS:000229071200011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLeung, WK=25224691800en_HK
dc.identifier.scopusauthoridNgai, VKS=8334953200en_HK
dc.identifier.scopusauthoridYau, JYY=7102167568en_HK
dc.identifier.scopusauthoridCheung, BPK=7103294773en_HK
dc.identifier.scopusauthoridTsang, PWK=8334953500en_HK
dc.identifier.scopusauthoridCorbet, EF=35609873200en_HK
dc.identifier.citeulike168432-

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