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Article: Interspecies variation in Candida biofilm formation studied using the Calgary biofilm device

TitleInterspecies variation in Candida biofilm formation studied using the Calgary biofilm device
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2006
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
Apmis, 2006, v. 114 n. 4, p. 298-306 How to Cite?
AbstractAn in vitro assay to study multiple Candida biofilms, in parallel, has been carried out using the Calgary biofilm device (CBD). We here report: i) standardization of the CBD for Candida albicans biofilm formation, ii) kinetics of C. albicans biofilm formation, iii) biofilm formation by five Candida species, and iv) effect of dietary carbohydrates on biofilm formation. The biofilm metabolic activity on all CBD pegs was similar (p=0.6693) and C. albicans biofilm formation revealed slow growth up to 36 h and significantly higher growth up to 48 h (p<0.001). Significant differences in total biofilm metabolic activity were seen for glucose, fructose and lactose grown C. albicans compared with sucrose and maltose grown yeasts. Candida krusei developed the largest biofilm mass (p<0.05) relative to C. albicans, C. glabrata, C. dubliniensis and C. tropicalis. Scanning electron microscopy revealed that C. krusei produced a thick multilayered biofilm of pseudohyphal forms embedded within the polymer matrix, whereas C. albicans, C. dubliniensis and C. tropicalis biofilms consisted of clusters or chains of cells with sparse extracellular matrix material. We conclude that CBD is a useful, simple, low cost miniature device for parallel study of Candida biofilms and factors modulating this phenomenon. © 2006 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/90724
ISSN
2015 Impact Factor: 1.933
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorParahitiyawa, NBen_HK
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorYe, Jen_HK
dc.contributor.authorTsang, PWKen_HK
dc.contributor.authorCheung, BPKen_HK
dc.contributor.authorYau, JYYen_HK
dc.contributor.authorYeung, SKWen_HK
dc.date.accessioned2010-09-17T10:07:21Z-
dc.date.available2010-09-17T10:07:21Z-
dc.date.issued2006en_HK
dc.identifier.citationApmis, 2006, v. 114 n. 4, p. 298-306en_HK
dc.identifier.issn0903-4641en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90724-
dc.description.abstractAn in vitro assay to study multiple Candida biofilms, in parallel, has been carried out using the Calgary biofilm device (CBD). We here report: i) standardization of the CBD for Candida albicans biofilm formation, ii) kinetics of C. albicans biofilm formation, iii) biofilm formation by five Candida species, and iv) effect of dietary carbohydrates on biofilm formation. The biofilm metabolic activity on all CBD pegs was similar (p=0.6693) and C. albicans biofilm formation revealed slow growth up to 36 h and significantly higher growth up to 48 h (p<0.001). Significant differences in total biofilm metabolic activity were seen for glucose, fructose and lactose grown C. albicans compared with sucrose and maltose grown yeasts. Candida krusei developed the largest biofilm mass (p<0.05) relative to C. albicans, C. glabrata, C. dubliniensis and C. tropicalis. Scanning electron microscopy revealed that C. krusei produced a thick multilayered biofilm of pseudohyphal forms embedded within the polymer matrix, whereas C. albicans, C. dubliniensis and C. tropicalis biofilms consisted of clusters or chains of cells with sparse extracellular matrix material. We conclude that CBD is a useful, simple, low cost miniature device for parallel study of Candida biofilms and factors modulating this phenomenon. © 2006 The Authors.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_HK
dc.relation.ispartofAPMISen_HK
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.subject.meshBiofilms - drug effects - growth & developmenten_HK
dc.subject.meshBioreactors - standardsen_HK
dc.subject.meshCandida - drug effects - physiology - ultrastructureen_HK
dc.subject.meshDietary Carbohydrates - pharmacologyen_HK
dc.subject.meshKineticsen_HK
dc.subject.meshMicroscopy, Electron, Scanningen_HK
dc.titleInterspecies variation in Candida biofilm formation studied using the Calgary biofilm deviceen_HK
dc.typeArticleen_HK
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.emailTsang, PWK:pwktsang@hku.hken_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityTsang, PWK=rp01388en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-0463.2006.apm_394.xen_HK
dc.identifier.pmid16689830-
dc.identifier.scopuseid_2-s2.0-33646521925en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646521925&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume114en_HK
dc.identifier.issue4en_HK
dc.identifier.spage298en_HK
dc.identifier.epage306en_HK
dc.identifier.eissn1600-0463-
dc.identifier.isiWOS:000237302500009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridParahitiyawa, NB=13408948800en_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridYe, J=23669624100en_HK
dc.identifier.scopusauthoridTsang, PWK=8334953500en_HK
dc.identifier.scopusauthoridCheung, BPK=7103294773en_HK
dc.identifier.scopusauthoridYau, JYY=7102167568en_HK
dc.identifier.scopusauthoridYeung, SKW=13408312500en_HK
dc.identifier.citeulike615041-

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