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Article: Cell density and cell aging as factors modulating antifungal resistance of Candida albicans biofilms

TitleCell density and cell aging as factors modulating antifungal resistance of Candida albicans biofilms
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2008
PublisherAmerican Society for Microbiology
Citation
Antimicrobial Agents And Chemotherapy, 2008, v. 52 n. 9, p. 3259-3266 How to Cite?
AbstractBiofilm formation is a major virulence attribute of Candida pathogenicity which contributes to higher antifungal resistance. We investigated the roles of cell density and cellular aging on the relative antifungal susceptibility of planktonic, biofilm, and biofilm-derived planktonic modes of Candida. A reference and a wild-type strain of Candida albicans were used to evaluate the MICs of caspofungin (CAS), amphotericin B (AMB), nystatin (NYT), ketoconazole (KTC), and flucytosine (5FC). Standard, NCCLS, and European Committee on Antibiotic Susceptibility Testing methods were used for planktonic MIC determination. Candida biofilms were then developed on polystyrene wells, and MICs were determined with a standard 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino)carbonyl]-2H-tetrazolium hydroxide assay. Subsequently, antifungal susceptibility testing was performed for greater inoculum concentrations and 24- and 48-h-old cultures of planktonic Candida. Furthermore, Candida biofilm-derived planktonic cells (BDPC) were also subjected to antifungal susceptibility testing. The MICs for both C. albicans strains in the planktonic mode were low, although on increasing the inoculum concentration (up to 1 × 108 cells/ml), a variable MIC was noted. On the contrary, for Candida biofilms, the MICs of antifungals were 15- to > 1,000-fold higher. Interestingly, the MICs for BDPC were lower and were similar to those for planktonic-mode cells, particularly those of CAS and AMB. Our data indicate that higher antifungal resistance of Candida biofilms is an intrinsic feature possibly related to the biofilm architecture rather than cellular density or cellular aging. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/90707
ISSN
2015 Impact Factor: 4.415
2015 SCImago Journal Rankings: 2.322
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong ResearchHKU 7624/06 M
Funding Information:

This work was supported by the Hong Kong Research Grants Council, RGC no. HKU 7624/06 M.

References

 

DC FieldValueLanguage
dc.contributor.authorSeneviratne, CJen_HK
dc.contributor.authorJin, LJen_HK
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-17T10:07:05Z-
dc.date.available2010-09-17T10:07:05Z-
dc.date.issued2008en_HK
dc.identifier.citationAntimicrobial Agents And Chemotherapy, 2008, v. 52 n. 9, p. 3259-3266en_HK
dc.identifier.issn0066-4804en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90707-
dc.description.abstractBiofilm formation is a major virulence attribute of Candida pathogenicity which contributes to higher antifungal resistance. We investigated the roles of cell density and cellular aging on the relative antifungal susceptibility of planktonic, biofilm, and biofilm-derived planktonic modes of Candida. A reference and a wild-type strain of Candida albicans were used to evaluate the MICs of caspofungin (CAS), amphotericin B (AMB), nystatin (NYT), ketoconazole (KTC), and flucytosine (5FC). Standard, NCCLS, and European Committee on Antibiotic Susceptibility Testing methods were used for planktonic MIC determination. Candida biofilms were then developed on polystyrene wells, and MICs were determined with a standard 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino)carbonyl]-2H-tetrazolium hydroxide assay. Subsequently, antifungal susceptibility testing was performed for greater inoculum concentrations and 24- and 48-h-old cultures of planktonic Candida. Furthermore, Candida biofilm-derived planktonic cells (BDPC) were also subjected to antifungal susceptibility testing. The MICs for both C. albicans strains in the planktonic mode were low, although on increasing the inoculum concentration (up to 1 × 108 cells/ml), a variable MIC was noted. On the contrary, for Candida biofilms, the MICs of antifungals were 15- to > 1,000-fold higher. Interestingly, the MICs for BDPC were lower and were similar to those for planktonic-mode cells, particularly those of CAS and AMB. Our data indicate that higher antifungal resistance of Candida biofilms is an intrinsic feature possibly related to the biofilm architecture rather than cellular density or cellular aging. Copyright © 2008, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiologyen_HK
dc.relation.ispartofAntimicrobial Agents and Chemotherapyen_HK
dc.rightsAntimicrobial Agents and Chemotherapy. Copyright © American Society for Microbiology.-
dc.subjectChemicals And Cas Registry Numbersen_HK
dc.titleCell density and cell aging as factors modulating antifungal resistance of Candida albicans biofilmsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0066-4804&volume=52&issue=9&spage=3259&epage=3266&date=2008&atitle=Cell+density+and+cell+aging+as+factors+modulating+antifungal+resistance+of+Candida+albicans+biofilm-
dc.identifier.emailSeneviratne, CJ: jaya@hku.hken_HK
dc.identifier.emailJin, LJ: ljjin@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, YH: hema@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.authoritySeneviratne, CJ=rp01372en_HK
dc.identifier.authorityJin, LJ=rp00028en_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/AAC.00541-08en_HK
dc.identifier.pmid18625775-
dc.identifier.pmcidPMC2533466-
dc.identifier.scopuseid_2-s2.0-50949124754en_HK
dc.identifier.hkuros150434-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-50949124754&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue9en_HK
dc.identifier.spage3259en_HK
dc.identifier.epage3266en_HK
dc.identifier.isiWOS:000258667300036-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSeneviratne, CJ=6701897753en_HK
dc.identifier.scopusauthoridJin, LJ=7403328850en_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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