Effect of residual water on dentin bond strength and hybridization of a one-bottle adhesive system

Abstract

This research investigated the effects of wet and dry conditions of phosphoric acid etched dentin on resin bonding and determined the optimum moisture condition for resin bonding using an ethanol-based one-bottle adhesive system. Bovine dentin surfaces were etched with 35% phosphoric acid and rinsed with water. Under four wet and dry conditions (overwet, blot dry, one-second dry and desiccated), resin composite was bonded using Single Bond. Tensile bond strength was measured and the results analyzed by one-way ANOVA and Fisher's PLSD test at the 5% level. The resin-dentin interfaces of bonded specimens were observed with SEM. The bond strength of overwet, blot dry, one-second dry and desiccated groups were 5.2 MPa, 12.6 MPa, 11.9 MPa and 4.4 Mpa, respectively. The blot dry group and one-second dry groups revealed significantly higher bond strengths than the desiccated and overwet groups (p<0.05). The formation of hybrid layers approximately 5 μm thick (overwet and blot dry), 2 pm (one-second dry) and 3 μm (desiccated) were observed. The coefficient of variation in the blot dry group was very high, even though a higher mean was observed. In the one-second dry group, the moisture content of the collagen network was possibly too low, such that hybrid layer formation was not as good even though the bond strength was high.