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Article: Role of oral streptococci in the pH-dependent carious dentin

TitleRole of oral streptococci in the pH-dependent carious dentin
Authors
KeywordsCarious dentin
Oral streptococci
PCR
pH
S. mutans
Issue Date2006
Citation
Journal Of Medical And Dental Sciences, 2006, v. 53 n. 3, p. 159-166 How to Cite?
AbstractThe aim of the present study was to investigate the extent to which oral streptococci or lactic acid producing bacteria are able to regulate pH value, especially at low pH values associated with dentinal carious activity using a PCR method. One millimeter-thick sections were sliced from 24 extracted carious human molars. The pH values on the tooth sections were evaluated using a pH-imaging microscope. A dentin sample (1X1X1mm) was prepared from the areas with the lowest or highest pH and homogenized to extract bacterial genomic DNA. Specific primers were used for nested PCR to mutans streptococci (MS: Streptococcus mutans and Streptococcus sobrinus). Conserved primers were also used for PCR to lactobacilli and gram positive bacteria. The PCR products were separated by electrophoresis, and then oral bacteria were identified. There was no significant difference between carious and intact dentin in MS identification. However, the frequency of the product ampli-fied by the conserved primers in carious dentin (16/24, 66.7%) was significantly higher than that in intact dentin (2/24, 8.3%), and PCR products demonstrated, by sequence analysis, various bacteria, including oral streptococci. It was concluded that oral streptococci may be associated with the development of "low pH-carious dentin".
Persistent Identifierhttp://hdl.handle.net/10722/90645
ISSN
2015 SCImago Journal Rankings: 0.210
References

 

DC FieldValueLanguage
dc.contributor.authorMaeda, Ten_HK
dc.contributor.authorKitasako, Yen_HK
dc.contributor.authorSenpuku, Hen_HK
dc.contributor.authorBurrow, MFen_HK
dc.contributor.authorTagami, Jen_HK
dc.date.accessioned2010-09-17T10:06:10Z-
dc.date.available2010-09-17T10:06:10Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Medical And Dental Sciences, 2006, v. 53 n. 3, p. 159-166en_HK
dc.identifier.issn1342-8810en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90645-
dc.description.abstractThe aim of the present study was to investigate the extent to which oral streptococci or lactic acid producing bacteria are able to regulate pH value, especially at low pH values associated with dentinal carious activity using a PCR method. One millimeter-thick sections were sliced from 24 extracted carious human molars. The pH values on the tooth sections were evaluated using a pH-imaging microscope. A dentin sample (1X1X1mm) was prepared from the areas with the lowest or highest pH and homogenized to extract bacterial genomic DNA. Specific primers were used for nested PCR to mutans streptococci (MS: Streptococcus mutans and Streptococcus sobrinus). Conserved primers were also used for PCR to lactobacilli and gram positive bacteria. The PCR products were separated by electrophoresis, and then oral bacteria were identified. There was no significant difference between carious and intact dentin in MS identification. However, the frequency of the product ampli-fied by the conserved primers in carious dentin (16/24, 66.7%) was significantly higher than that in intact dentin (2/24, 8.3%), and PCR products demonstrated, by sequence analysis, various bacteria, including oral streptococci. It was concluded that oral streptococci may be associated with the development of "low pH-carious dentin".en_HK
dc.languageengen_HK
dc.relation.ispartofJournal of Medical and Dental Sciencesen_HK
dc.subjectCarious dentinen_HK
dc.subjectOral streptococcien_HK
dc.subjectPCRen_HK
dc.subjectpHen_HK
dc.subjectS. mutansen_HK
dc.titleRole of oral streptococci in the pH-dependent carious dentinen_HK
dc.typeArticleen_HK
dc.identifier.emailBurrow, MF:mfburr58@hku.hken_HK
dc.identifier.authorityBurrow, MF=rp01306en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-34249020530en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34249020530&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume53en_HK
dc.identifier.issue3en_HK
dc.identifier.spage159en_HK
dc.identifier.epage166en_HK
dc.identifier.scopusauthoridMaeda, T=37043787400en_HK
dc.identifier.scopusauthoridKitasako, Y=7003435929en_HK
dc.identifier.scopusauthoridSenpuku, H=7003409740en_HK
dc.identifier.scopusauthoridBurrow, MF=7005876730en_HK
dc.identifier.scopusauthoridTagami, J=7005967527en_HK

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