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Article: Photodynamic inactivation of Candida albicans by BAM-SiPc: Original article

TitlePhotodynamic inactivation of Candida albicans by BAM-SiPc: Original article
Authors
KeywordsAntifungal drug
Photodynamic therapy
Photosensitiser
Phthalocyanine
Issue Date2010
PublisherWiley-Blackwell Verlag GmbH. The Journal's web site is located at http://www.blackwellpublishing.com/journals/MYC
Citation
Mycoses, 2010, v. 53 n. 3, p. 215-220 How to Cite?
AbstractPhotodynamic therapy is a treatment that combines the use of three non-toxic components, viz. photosensitiser, light and oxygen to cause localised oxidative photodamage. In the present study, the antifungal effect of the photosensitiser, BAM-SiPc, an unsymmetrical bisamino phthalocyanine, was investigated. BAM-SiPc was effective in photo-inactivating Candida albicans in a dose-dependent manner. The cell viability as determined by the clonogenic assay was reduced to c. 10% at 0.02 μmol l -1 BAM-SiPc with a total fluence of 12 J cm -2 at a cell density of 10 7 cells ml -1. A short incubation time of 5-15 min was sufficient to allow the photosensitiser to exert its optimal antifungal activity. Microscopical analysis showed that BAM-SiPc was effectively internalised by the fungal cells. Photodynamic treatment led to an increase in the intracellular reactive oxygen species level and disturbed the membrane integrity of the fungal cells. © 2009 Blackwell Verlag GmbH.
Persistent Identifierhttp://hdl.handle.net/10722/90643
ISSN
2015 Impact Factor: 2.332
2015 SCImago Journal Rankings: 0.921
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative RegionCUHK4569/05M
Funding Information:

We thank Prof. Dennis K.P. Ng, Department of Chemistry, The Chinese University of Hong Kong, for providing the photosensitiser BAM-SiPc and helpful discussion. This work was supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, Project No. CUHK4569/05M.

References

 

DC FieldValueLanguage
dc.contributor.authorSo, CWen_HK
dc.contributor.authorTsang, PWKen_HK
dc.contributor.authorLo, PCen_HK
dc.contributor.authorSeneviratne, CJen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorFong, WPen_HK
dc.date.accessioned2010-09-17T10:06:08Z-
dc.date.available2010-09-17T10:06:08Z-
dc.date.issued2010en_HK
dc.identifier.citationMycoses, 2010, v. 53 n. 3, p. 215-220en_HK
dc.identifier.issn0933-7407en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90643-
dc.description.abstractPhotodynamic therapy is a treatment that combines the use of three non-toxic components, viz. photosensitiser, light and oxygen to cause localised oxidative photodamage. In the present study, the antifungal effect of the photosensitiser, BAM-SiPc, an unsymmetrical bisamino phthalocyanine, was investigated. BAM-SiPc was effective in photo-inactivating Candida albicans in a dose-dependent manner. The cell viability as determined by the clonogenic assay was reduced to c. 10% at 0.02 μmol l -1 BAM-SiPc with a total fluence of 12 J cm -2 at a cell density of 10 7 cells ml -1. A short incubation time of 5-15 min was sufficient to allow the photosensitiser to exert its optimal antifungal activity. Microscopical analysis showed that BAM-SiPc was effectively internalised by the fungal cells. Photodynamic treatment led to an increase in the intracellular reactive oxygen species level and disturbed the membrane integrity of the fungal cells. © 2009 Blackwell Verlag GmbH.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Verlag GmbH. The Journal's web site is located at http://www.blackwellpublishing.com/journals/MYCen_HK
dc.relation.ispartofMycosesen_HK
dc.rightsThe definitive version is available at www3.interscience.wiley.com-
dc.subjectAntifungal drugen_HK
dc.subjectPhotodynamic therapyen_HK
dc.subjectPhotosensitiseren_HK
dc.subjectPhthalocyanineen_HK
dc.subject.meshAntifungal Agents - pharmacology-
dc.subject.meshCandida albicans - chemistry - drug effects-
dc.subject.meshIndoles - pharmacology-
dc.subject.meshOrganosilicon Compounds - pharmacology-
dc.subject.meshPhotosensitizing Agents - pharmacology-
dc.titlePhotodynamic inactivation of Candida albicans by BAM-SiPc: Original articleen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0933-7407&volume=53&issue=3&spage=215&epage=220&date=2010&atitle=Photodynamic+inactivation+of+Candida+albicans+by+BAM-SiPc-
dc.identifier.emailTsang, PWK:pwktsang@hku.hken_HK
dc.identifier.emailSeneviratne, CJ:jaya@hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authorityTsang, PWK=rp01388en_HK
dc.identifier.authoritySeneviratne, CJ=rp01372en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1439-0507.2008.01688.xen_HK
dc.identifier.pmid19298354-
dc.identifier.scopuseid_2-s2.0-77950835056en_HK
dc.identifier.hkuros175344-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77950835056&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume53en_HK
dc.identifier.issue3en_HK
dc.identifier.spage215en_HK
dc.identifier.epage220en_HK
dc.identifier.isiWOS:000276467000006-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridSo, CW=36437729900en_HK
dc.identifier.scopusauthoridTsang, PWK=8334953500en_HK
dc.identifier.scopusauthoridLo, PC=7004401819en_HK
dc.identifier.scopusauthoridSeneviratne, CJ=6701897753en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridFong, WP=7102816006en_HK
dc.identifier.citeulike7067813-

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