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Article: GroEL-GroES solubilizes abundantly expressed xylulokinase in Escherichia coli

TitleGroEL-GroES solubilizes abundantly expressed xylulokinase in Escherichia coli
Authors
KeywordsSpecies Index: Bacillus Megaterium
Escherichia Coli
Negibacteria
Issue Date2005
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JAM
Citation
Journal Of Applied Microbiology, 2005, v. 98 n. 1, p. 210-215 How to Cite?
AbstractAims: The aims of the present work were to solubilize the abundantly expressed recombinant xylulokinase in Escherichia coli and to develop a reliable xylulokinase assay. Methods and Results: Three mutants of xylulokinase of Bacillus megaterium that were expressed at high level but formed insoluble protein in E. coli BL21(DE3)pLysS were selected for solubility study. The solubility of xylulokinase increased eight to 77-fold after introduction of molecular chaperones GroEL-GroES into the host. Conclusion: This investigation reports that GroEL-GroES minimizes the formation of insoluble protein in three highly expressed recombinant xylulokinases and an improved xylulokinase assay. Significance and Impact of the Study: Commercial production of bioethanol is critically dependent on the development of an efficient and low-cost process of enzymatic conversion of xylan, a major component in lignocellulose biomass, to xylulose-5-phosphate, which can then be channelled into pentose phosphate pathway and metabolized to ethanol. The improved intracellular xylulokinase activity is expected to facilitate the xylose degradation.
Persistent Identifierhttp://hdl.handle.net/10722/90639
ISSN
2015 Impact Factor: 2.156
2015 SCImago Journal Rankings: 0.921
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorBu, Sen_HK
dc.contributor.authorTsang, PWKen_HK
dc.contributor.authorFu, RZen_HK
dc.date.accessioned2010-09-17T10:06:05Z-
dc.date.available2010-09-17T10:06:05Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Applied Microbiology, 2005, v. 98 n. 1, p. 210-215en_HK
dc.identifier.issn1364-5072en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90639-
dc.description.abstractAims: The aims of the present work were to solubilize the abundantly expressed recombinant xylulokinase in Escherichia coli and to develop a reliable xylulokinase assay. Methods and Results: Three mutants of xylulokinase of Bacillus megaterium that were expressed at high level but formed insoluble protein in E. coli BL21(DE3)pLysS were selected for solubility study. The solubility of xylulokinase increased eight to 77-fold after introduction of molecular chaperones GroEL-GroES into the host. Conclusion: This investigation reports that GroEL-GroES minimizes the formation of insoluble protein in three highly expressed recombinant xylulokinases and an improved xylulokinase assay. Significance and Impact of the Study: Commercial production of bioethanol is critically dependent on the development of an efficient and low-cost process of enzymatic conversion of xylan, a major component in lignocellulose biomass, to xylulose-5-phosphate, which can then be channelled into pentose phosphate pathway and metabolized to ethanol. The improved intracellular xylulokinase activity is expected to facilitate the xylose degradation.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JAMen_HK
dc.relation.ispartofJournal of Applied Microbiologyen_HK
dc.subjectSpecies Index: Bacillus Megateriumen_HK
dc.subjectEscherichia Colien_HK
dc.subjectNegibacteriaen_HK
dc.subject.meshBioreactorsen_HK
dc.subject.meshChaperonin 10 - metabolismen_HK
dc.subject.meshChaperonin 60 - metabolismen_HK
dc.subject.meshEscherichia coli - enzymologyen_HK
dc.subject.meshFermentationen_HK
dc.subject.meshMolecular Chaperonesen_HK
dc.subject.meshPentosephosphates - metabolismen_HK
dc.subject.meshPhosphotransferases (Alcohol Group Acceptor) - analysis - metabolismen_HK
dc.subject.meshRecombinant Proteins - metabolismen_HK
dc.subject.meshXylans - metabolismen_HK
dc.subject.meshXylulose - metabolismen_HK
dc.titleGroEL-GroES solubilizes abundantly expressed xylulokinase in Escherichia colien_HK
dc.typeArticleen_HK
dc.identifier.emailTsang, PWK:pwktsang@hku.hken_HK
dc.identifier.authorityTsang, PWK=rp01388en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1365-2672.2004.02446.xen_HK
dc.identifier.pmid15610434-
dc.identifier.scopuseid_2-s2.0-12344257694en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-12344257694&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume98en_HK
dc.identifier.issue1en_HK
dc.identifier.spage210en_HK
dc.identifier.epage215en_HK
dc.identifier.isiWOS:000225791600023-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridBu, S=16634803200en_HK
dc.identifier.scopusauthoridTsang, PWK=8334953500en_HK
dc.identifier.scopusauthoridFu, RZ=7203054356en_HK
dc.identifier.citeulike38401-

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