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Article: SDF1-alpha is associated with VEGFR-2 in human choroidal neovascularisation

TitleSDF1-alpha is associated with VEGFR-2 in human choroidal neovascularisation
Authors
KeywordsChoroidal neovascularisation
Endothelial progenitor cells
Stromal cell derived factor 1-alpha
Issue Date2008
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ymvre
Citation
Microvascular Research, 2008, v. 75 n. 3, p. 302-307 How to Cite?
AbstractEndothelial progenitor cells (EPCs) have been shown to contribute to experimentally induced choroidal neovascularisation (CNV) in animal models. The recruitment pathway for EPCs is dependent on the chemokine stromal cell derived factor 1-alpha (SDF) and its receptor CXCR4 on the progenitor cell. We examined 23 specimens of CNV occurring secondary to a variety of aetiologies (10 secondary to age-related macular degeneration (AMD), 4 inflammatory, 4 idiopathic and 5 melanoma-associated) for the presence and distribution of SDF and CXCR4 in order to determine if this pathway may play a role in neovascularisation. Specimens were examined by immunohistochemistry using a panel of antibodies against SDF, CXCR4, vascular endothelial growth factor receptor 2 (VEGFR-2), CD34 (endothelial cells), CD68 (macrophages) and cytokeratins (retinal pigment epithelium; RPE). SDF was detected in 2 cases of CNV in AMD, 1 inflammatory CNV, 3 idiopathic CNVs and in 3 cases of CNV associated with melanoma. A significant association was found between SDF and VEGFR-2 immunostaining in individual membranes (p < 0.001). Localisation of SDF immunostaining to the presumed RPE was also significant (p < 0.05). CXCR4 immunostaining was widespread in all membranes in keeping with the published work of other investigators. Our study suggests that SDF, which may be produced by the RPE, could play a role in CNV. © 2007 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/90364
ISSN
2015 Impact Factor: 2.3
2015 SCImago Journal Rankings: 1.030
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuerin, Een_HK
dc.contributor.authorSheridan, Cen_HK
dc.contributor.authorAssheton, Den_HK
dc.contributor.authorKent, Den_HK
dc.contributor.authorWong, Den_HK
dc.contributor.authorGrant, Men_HK
dc.contributor.authorHiscott, Pen_HK
dc.date.accessioned2010-09-06T10:09:21Z-
dc.date.available2010-09-06T10:09:21Z-
dc.date.issued2008en_HK
dc.identifier.citationMicrovascular Research, 2008, v. 75 n. 3, p. 302-307en_HK
dc.identifier.issn0026-2862en_HK
dc.identifier.urihttp://hdl.handle.net/10722/90364-
dc.description.abstractEndothelial progenitor cells (EPCs) have been shown to contribute to experimentally induced choroidal neovascularisation (CNV) in animal models. The recruitment pathway for EPCs is dependent on the chemokine stromal cell derived factor 1-alpha (SDF) and its receptor CXCR4 on the progenitor cell. We examined 23 specimens of CNV occurring secondary to a variety of aetiologies (10 secondary to age-related macular degeneration (AMD), 4 inflammatory, 4 idiopathic and 5 melanoma-associated) for the presence and distribution of SDF and CXCR4 in order to determine if this pathway may play a role in neovascularisation. Specimens were examined by immunohistochemistry using a panel of antibodies against SDF, CXCR4, vascular endothelial growth factor receptor 2 (VEGFR-2), CD34 (endothelial cells), CD68 (macrophages) and cytokeratins (retinal pigment epithelium; RPE). SDF was detected in 2 cases of CNV in AMD, 1 inflammatory CNV, 3 idiopathic CNVs and in 3 cases of CNV associated with melanoma. A significant association was found between SDF and VEGFR-2 immunostaining in individual membranes (p < 0.001). Localisation of SDF immunostaining to the presumed RPE was also significant (p < 0.05). CXCR4 immunostaining was widespread in all membranes in keeping with the published work of other investigators. Our study suggests that SDF, which may be produced by the RPE, could play a role in CNV. © 2007 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ymvreen_HK
dc.relation.ispartofMicrovascular Researchen_HK
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in <Journal title>. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, [VOL#, ISSUE#, (DATE)] DOI#-
dc.subjectChoroidal neovascularisationen_HK
dc.subjectEndothelial progenitor cellsen_HK
dc.subjectStromal cell derived factor 1-alphaen_HK
dc.subject.meshChemokine CXCL12 - metabolism-
dc.subject.meshChoroidal Neovascularization - metabolism - pathology-
dc.subject.meshEndothelium, Vascular - metabolism - pathology-
dc.subject.meshMesenchymal Stem Cells - metabolism - pathology-
dc.subject.meshReceptors, CXCR4 - metabolism-
dc.titleSDF1-alpha is associated with VEGFR-2 in human choroidal neovascularisationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0026-2862&volume=75&issue=3&spage=302&epage=307&date=2008&atitle=SDF1-alpha+is+associated+with+VEGFR-2+in+human+choroidal+neovascularisationen_HK
dc.identifier.emailWong, D: shdwong@hku.hken_HK
dc.identifier.authorityWong, D=rp00516en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.mvr.2007.12.001en_HK
dc.identifier.pmid18234239-
dc.identifier.scopuseid_2-s2.0-41949119693en_HK
dc.identifier.hkuros152802en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-41949119693&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume75en_HK
dc.identifier.issue3en_HK
dc.identifier.spage302en_HK
dc.identifier.epage307en_HK
dc.identifier.isiWOS:000256198800002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGuerin, E=13608875400en_HK
dc.identifier.scopusauthoridSheridan, C=7004974390en_HK
dc.identifier.scopusauthoridAssheton, D=15839036200en_HK
dc.identifier.scopusauthoridKent, D=35512364500en_HK
dc.identifier.scopusauthoridWong, D=7401536078en_HK
dc.identifier.scopusauthoridGrant, M=7401439724en_HK
dc.identifier.scopusauthoridHiscott, P=7006368693en_HK

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