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Article: Direct visualization of mouse brain oxygen distribution by electron paramagnetic resonance imaging: Application to focal cerebral ischemia

TitleDirect visualization of mouse brain oxygen distribution by electron paramagnetic resonance imaging: Application to focal cerebral ischemia
Authors
KeywordsElectron paramagnetic resonance imaging
Nitroxides
Oxygen
Stroke
Issue Date2009
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/jcbfm
Citation
Journal Of Cerebral Blood Flow And Metabolism, 2009, v. 29 n. 10, p. 1695-1703 How to Cite?
AbstractElectron paramagnetic resonance imaging (EPRI) is a new modality for visualizing O 2 distribution in tissues, such as the brain after stroke or after administration of drugs of abuse. We have recently shown that 3-acetoxymethoxycarbonyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl 1 is a pro-imaging agent that can cross the blood-brain barrier. After hydrolysis by esterases, the anion of 3-carboxy-2,2,5,5-tetramethyl-1-tetramethyl-1- pyrrolidinyloxyl 2 is trapped in brain tissue. In this study, we investigated the feasibility of using this to map the changes of O 2 concentration in mouse brain after focal ischemia. The decrease in tissue O 2 concentration in the ischemic region of mouse brain was clearly visualized by EPRI. The hypoxic zone mapped by EPRI was spatially well correlated with the infarction area in the brain imaged by diffusion-weighted magnetic resonance imaging (MRI). Finally, we observed a decrease in the size of the hypoxic region when the mouse breathed higher levels of O 2. This finding suggests that EPRI with specifically designed nitroxides is a promising imaging modality for visualizing O 2 distribution in brain tissue, especially in an ischemic brain. We believe that this imaging method can be used for monitoring the effects of therapeutic intervention aimed at enhancing brain O 2 supply, which is crucial in minimizing brain injury after stroke. © 2009 ISCBFM All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/89677
ISSN
2015 Impact Factor: 4.929
2015 SCImago Journal Rankings: 3.004
ISI Accession Number ID
Funding AgencyGrant Number
National Institutes of HealthRR15636
AG031725
RGC GRF774408M
EB2034
DA023473
GM56481
Funding Information:

This research was supported in part by grants from the National Institutes of Health, RR15636 and AG031725 (KJL), RGC GRF No. 774408M (JGS), EB2034 and DA023473 (GMR), and GM56481 (JPYK). The authors thank Bruker BioSpin, Inc for technological support.

References

 

DC FieldValueLanguage
dc.contributor.authorShen, Jen_HK
dc.contributor.authorSood, Ren_HK
dc.contributor.authorWeaver, Jen_HK
dc.contributor.authorTimmins, GSen_HK
dc.contributor.authorSchnell, Aen_HK
dc.contributor.authorMiyake, Men_HK
dc.contributor.authorKao, JPYen_HK
dc.contributor.authorRosen, GMen_HK
dc.contributor.authorLiu, KJen_HK
dc.date.accessioned2010-09-06T10:00:19Z-
dc.date.available2010-09-06T10:00:19Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Cerebral Blood Flow And Metabolism, 2009, v. 29 n. 10, p. 1695-1703en_HK
dc.identifier.issn0271-678Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/89677-
dc.description.abstractElectron paramagnetic resonance imaging (EPRI) is a new modality for visualizing O 2 distribution in tissues, such as the brain after stroke or after administration of drugs of abuse. We have recently shown that 3-acetoxymethoxycarbonyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl 1 is a pro-imaging agent that can cross the blood-brain barrier. After hydrolysis by esterases, the anion of 3-carboxy-2,2,5,5-tetramethyl-1-tetramethyl-1- pyrrolidinyloxyl 2 is trapped in brain tissue. In this study, we investigated the feasibility of using this to map the changes of O 2 concentration in mouse brain after focal ischemia. The decrease in tissue O 2 concentration in the ischemic region of mouse brain was clearly visualized by EPRI. The hypoxic zone mapped by EPRI was spatially well correlated with the infarction area in the brain imaged by diffusion-weighted magnetic resonance imaging (MRI). Finally, we observed a decrease in the size of the hypoxic region when the mouse breathed higher levels of O 2. This finding suggests that EPRI with specifically designed nitroxides is a promising imaging modality for visualizing O 2 distribution in brain tissue, especially in an ischemic brain. We believe that this imaging method can be used for monitoring the effects of therapeutic intervention aimed at enhancing brain O 2 supply, which is crucial in minimizing brain injury after stroke. © 2009 ISCBFM All rights reserved.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/jcbfmen_HK
dc.relation.ispartofJournal of Cerebral Blood Flow and Metabolismen_HK
dc.subjectElectron paramagnetic resonance imagingen_HK
dc.subjectNitroxidesen_HK
dc.subjectOxygenen_HK
dc.subjectStrokeen_HK
dc.titleDirect visualization of mouse brain oxygen distribution by electron paramagnetic resonance imaging: Application to focal cerebral ischemiaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0271-678X&volume=29&spage=1695&epage=1703.&date=2009&atitle=Direct+visualization+of+mouse+brain+oxygen+distribution+by+electron+paramagnetic+resonance+imaging:+Application+to+focal+cerebral+ischemia.en_HK
dc.identifier.emailShen, J: shenjg@hku.hken_HK
dc.identifier.authorityShen, J=rp00487en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/jcbfm.2009.89en_HK
dc.identifier.pmid19675560-
dc.identifier.scopuseid_2-s2.0-70349684852en_HK
dc.identifier.hkuros158832en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70349684852&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1695en_HK
dc.identifier.epage1703en_HK
dc.identifier.eissn1559-7016-
dc.identifier.isiWOS:000270479600007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShen, J=7404929947en_HK
dc.identifier.scopusauthoridSood, R=11141778600en_HK
dc.identifier.scopusauthoridWeaver, J=7402079019en_HK
dc.identifier.scopusauthoridTimmins, GS=36553712100en_HK
dc.identifier.scopusauthoridSchnell, A=35119562100en_HK
dc.identifier.scopusauthoridMiyake, M=7201843244en_HK
dc.identifier.scopusauthoridKao, JPY=7201375605en_HK
dc.identifier.scopusauthoridRosen, GM=35497253700en_HK
dc.identifier.scopusauthoridLiu, KJ=7404200456en_HK
dc.identifier.citeulike8158484-

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