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Article: A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region

TitleA DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region
Authors
Keywords5 S ribosomal DNA gene
Authentication by DNA microarray
Chinese medicine
Dendrobium officinale (Fengdu Shihu, FDSH)
Identification of plant adulterants
Issue Date2008
PublisherPortland Press Ltd. The Journal's web site is located at http://www.babonline.org
Citation
Biotechnology And Applied Biochemistry, 2008, v. 49 n. 2, p. 149-154 How to Cite?
AbstractA DNA microarray was constructed for high-throughput identification of the plant resource of commercial FDSH [Fengdu Shihu (Dendrobium officinale)]. The 5 S rDNA (ribosomal DNA) intergenic spacer region in D. officinale, D. nobile, D. moniliforme, D. hercoglossum, D. williamsonii, D. capillipes, D. wilsonii and D. jenkinsii was amplified by a single primer pair and sequenced. The sequences showed polymorphism. They were incorporated on a glass slide and hybridized with fluorescently labelled 5 S sequences from commercial Shihu. The DNA microarray enabled the differentiation of D. officinale from the other species tested. FDSH could thus be distinguished from its adulterants. It is evident that DNA microarrays provide a high-throughput and reliable approach for the identification of plant resources, and the method presented here is useful for the authentication of FDSH. © 2008 Portland Press Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/89666
ISSN
2015 Impact Factor: 1.429
2015 SCImago Journal Rankings: 0.415
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSze, SCWen_HK
dc.contributor.authorZhang, KYBen_HK
dc.contributor.authorShaw, PCen_HK
dc.contributor.authorBut, PPHen_HK
dc.contributor.authorNg, TBen_HK
dc.contributor.authorTong, Yen_HK
dc.date.accessioned2010-09-06T10:00:09Z-
dc.date.available2010-09-06T10:00:09Z-
dc.date.issued2008en_HK
dc.identifier.citationBiotechnology And Applied Biochemistry, 2008, v. 49 n. 2, p. 149-154en_HK
dc.identifier.issn0885-4513en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89666-
dc.description.abstractA DNA microarray was constructed for high-throughput identification of the plant resource of commercial FDSH [Fengdu Shihu (Dendrobium officinale)]. The 5 S rDNA (ribosomal DNA) intergenic spacer region in D. officinale, D. nobile, D. moniliforme, D. hercoglossum, D. williamsonii, D. capillipes, D. wilsonii and D. jenkinsii was amplified by a single primer pair and sequenced. The sequences showed polymorphism. They were incorporated on a glass slide and hybridized with fluorescently labelled 5 S sequences from commercial Shihu. The DNA microarray enabled the differentiation of D. officinale from the other species tested. FDSH could thus be distinguished from its adulterants. It is evident that DNA microarrays provide a high-throughput and reliable approach for the identification of plant resources, and the method presented here is useful for the authentication of FDSH. © 2008 Portland Press Ltd.en_HK
dc.languageengen_HK
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.babonline.orgen_HK
dc.relation.ispartofBiotechnology and Applied Biochemistryen_HK
dc.subject5 S ribosomal DNA geneen_HK
dc.subjectAuthentication by DNA microarrayen_HK
dc.subjectChinese medicineen_HK
dc.subjectDendrobium officinale (Fengdu Shihu, FDSH)en_HK
dc.subjectIdentification of plant adulterantsen_HK
dc.titleA DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer regionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0885-4513&volume=49&spage=149&epage=154&date=2008&atitle=A+DNA+microarray+for+differentiation+of+the+Chinese+medicinal+herb+Dendrobium+officinale+(Fengdou+Shihu)+by+its+5+S+ribosomal+DNA+intergenic+spacer+regionen_HK
dc.identifier.emailSze, SCW: stephens@hku.hken_HK
dc.identifier.emailZhang, KYB: ybzhang@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.authoritySze, SCW=rp00514en_HK
dc.identifier.authorityZhang, KYB=rp01410en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1042/BA20070059en_HK
dc.identifier.pmid17680776-
dc.identifier.scopuseid_2-s2.0-38749130871en_HK
dc.identifier.hkuros141891en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-38749130871&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume49en_HK
dc.identifier.issue2en_HK
dc.identifier.spage149en_HK
dc.identifier.epage154en_HK
dc.identifier.isiWOS:000253080900008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSze, SCW=23482617000en_HK
dc.identifier.scopusauthoridZhang, KYB=23483121900en_HK
dc.identifier.scopusauthoridShaw, PC=35599523600en_HK
dc.identifier.scopusauthoridBut, PPH=7005593430en_HK
dc.identifier.scopusauthoridNg, TB=35311803300en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK

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