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Article: Quality assessment of Cortex phellodendri by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry

TitleQuality assessment of Cortex phellodendri by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry
Authors
KeywordsCortex phellodendri
HPLC-UV-ESI/MS
Limonoidal triterpenes
Phenols
Quality assessment
Quaternary isoquinoline alkaloids
Traditional herbal medicine (THM)
Issue Date2010
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0269-3879/
Citation
Biomedical Chromatography, 2010, v. 24 n. 4, p. 438-453 How to Cite?
AbstractA simple method based on liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI-MS) was developed for the quality assessment of Cortex Phellodendri (CP), which was mainly derived from two species of Phellodendron chinense Schneid and Phellodendron amurense Rupr. Total 41 compounds, including 14 phenols, 24 alkaloids and three liminoidal triterpenes were identified or tentatively characterized from the 75% methanol extract of CP samples by online ESI-MS n fragmentation and UV spectra analysis. Among them, two phenols and six alkaloids were simultaneously quantified using HPLC-DAD method. The validated HPLC-DAD method showed a good linearity, precision, repeatability and accuracy for the quantification of eight marker compounds. Furthermore, the plausible fragmentation pathway of the representative compounds were proposed in the present study. The differences of the chemical constituents content and the comprehensive HPLC profiles between the two CP species using LC-DAD-ESI-MS method are reported for the first time, indicating that the CP drugs from different resources should be used separately in the clinic. Copyright © 2009 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/89638
ISSN
2015 Impact Factor: 1.729
2015 SCImago Journal Rankings: 0.572
ISI Accession Number ID
Funding AgencyGrant Number
Committee on Research and Conference Grants200807176144
Seed Funding Programme for Applied Research200802160025
University of Hong Kong
Funding Information:

This research was supported by the Small Project Funding (200807176144) from the Committee on Research and Conference Grants, Seed Funding Programme for Applied Research (no. 200802160025), the University of Hong Kong. The authors are also grateful to the Physical and Chemical Testing Center in the Jin Nan University, Guangzhou, for assistance with NMR and ESIMS experiment.

References

 

DC FieldValueLanguage
dc.contributor.authorHu, YMen_HK
dc.contributor.authorSu, GHen_HK
dc.contributor.authorSze, SCWen_HK
dc.contributor.authorYe, Wen_HK
dc.contributor.authorTong, Yen_HK
dc.date.accessioned2010-09-06T09:59:44Z-
dc.date.available2010-09-06T09:59:44Z-
dc.date.issued2010en_HK
dc.identifier.citationBiomedical Chromatography, 2010, v. 24 n. 4, p. 438-453en_HK
dc.identifier.issn0269-3879en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89638-
dc.description.abstractA simple method based on liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI-MS) was developed for the quality assessment of Cortex Phellodendri (CP), which was mainly derived from two species of Phellodendron chinense Schneid and Phellodendron amurense Rupr. Total 41 compounds, including 14 phenols, 24 alkaloids and three liminoidal triterpenes were identified or tentatively characterized from the 75% methanol extract of CP samples by online ESI-MS n fragmentation and UV spectra analysis. Among them, two phenols and six alkaloids were simultaneously quantified using HPLC-DAD method. The validated HPLC-DAD method showed a good linearity, precision, repeatability and accuracy for the quantification of eight marker compounds. Furthermore, the plausible fragmentation pathway of the representative compounds were proposed in the present study. The differences of the chemical constituents content and the comprehensive HPLC profiles between the two CP species using LC-DAD-ESI-MS method are reported for the first time, indicating that the CP drugs from different resources should be used separately in the clinic. Copyright © 2009 John Wiley & Sons, Ltd.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0269-3879/en_HK
dc.relation.ispartofBiomedical Chromatographyen_HK
dc.rightsBiomedical Chromatography. Copyright © John Wiley & Sons Ltd.en_HK
dc.subjectCortex phellodendrien_HK
dc.subjectHPLC-UV-ESI/MSen_HK
dc.subjectLimonoidal triterpenesen_HK
dc.subjectPhenolsen_HK
dc.subjectQuality assessmenten_HK
dc.subjectQuaternary isoquinoline alkaloidsen_HK
dc.subjectTraditional herbal medicine (THM)en_HK
dc.titleQuality assessment of Cortex phellodendri by high-performance liquid chromatography coupled with electrospray ionization mass spectrometryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0269-3879&volume=&spage=&epage=&date=2009&atitle=Quality+Assessment+Of+Cortex+Phellodendri+By+High-performance+Liquid+Chromatography+Coupled+With+Electrospray+Ionization+Mass+Spectrometryen_HK
dc.identifier.emailSze, SCW: stephens@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.authoritySze, SCW=rp00514en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/bmc.1311en_HK
dc.identifier.pmid19711298-
dc.identifier.scopuseid_2-s2.0-77950202791en_HK
dc.identifier.hkuros160700en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77950202791&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue4en_HK
dc.identifier.spage438en_HK
dc.identifier.epage453en_HK
dc.identifier.isiWOS:000276169100015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHu, YM=7407116890en_HK
dc.identifier.scopusauthoridSu, GH=36086803700en_HK
dc.identifier.scopusauthoridSze, SCW=23482617000en_HK
dc.identifier.scopusauthoridYe, W=7201560816en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK

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