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Article: Isolation and characterization of the phytoene desaturase gene as a potential selective marker for genetic engineering of the astaxanthin-producing green alga Chlorella zofingiensis (Chlorophyta)

TitleIsolation and characterization of the phytoene desaturase gene as a potential selective marker for genetic engineering of the astaxanthin-producing green alga Chlorella zofingiensis (Chlorophyta)
Authors
KeywordsAstaxanthin
Chlorella zofingiensis
Herbicide
Phytoene desaturase
Point mutation
Issue Date2008
PublisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPY
Citation
Journal Of Phycology, 2008, v. 44 n. 3, p. 684-690 How to Cite?
AbstractPhytoene desaturase (PDS) is a rate-limiting enzyme in carotenoid biosynthesis. Algal PDS is inhibited by some herbicides, leading to the bleaching of the cells due to destruction of chl. Specific point mutations in PDS confer resistance to the herbicide norflurazon, suggesting that mutated PDS could be used as a dominant selectable marker for genetic engineering of algae, for which very few selective markers are available. In this study, we report the isolation and characterization of the PDS gene from the astaxanthin-producing green alga Chlorella zofingiensis Dönz. The open reading frame (ORF) of this PDS gene, interrupted by six introns, encoded a polypeptide of 558 amino acid residues. The deduced protein sequence showed significant homology to phytoene desaturases of algae, cyanobacteria, and higher plants. Expression of the PDS gene in Escherichia coli demonstrated that the enzyme was able to convert phytoene to ζ-carotene. The PDS gene in Chlorella was shown to be up-regulated by high light and glucose treatment. With a single amino acid change (L516R), the mutated PDS-L516R was still active and exhibited ∼36-fold greater resistance to the bleaching herbicide norflurazon than the unaltered enzyme. Thus, the modified PDS gene could be a useful tool for genetic engineering of carotenoid biosynthesis in C. zofingiensis and perhaps also in other algae. © 2008 Phycological Society of America.
Persistent Identifierhttp://hdl.handle.net/10722/89319
ISSN
2015 Impact Factor: 2.536
2015 SCImago Journal Rankings: 1.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, Jen_HK
dc.contributor.authorLiu, Jen_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T09:55:21Z-
dc.date.available2010-09-06T09:55:21Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Phycology, 2008, v. 44 n. 3, p. 684-690en_HK
dc.identifier.issn0022-3646en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89319-
dc.description.abstractPhytoene desaturase (PDS) is a rate-limiting enzyme in carotenoid biosynthesis. Algal PDS is inhibited by some herbicides, leading to the bleaching of the cells due to destruction of chl. Specific point mutations in PDS confer resistance to the herbicide norflurazon, suggesting that mutated PDS could be used as a dominant selectable marker for genetic engineering of algae, for which very few selective markers are available. In this study, we report the isolation and characterization of the PDS gene from the astaxanthin-producing green alga Chlorella zofingiensis Dönz. The open reading frame (ORF) of this PDS gene, interrupted by six introns, encoded a polypeptide of 558 amino acid residues. The deduced protein sequence showed significant homology to phytoene desaturases of algae, cyanobacteria, and higher plants. Expression of the PDS gene in Escherichia coli demonstrated that the enzyme was able to convert phytoene to ζ-carotene. The PDS gene in Chlorella was shown to be up-regulated by high light and glucose treatment. With a single amino acid change (L516R), the mutated PDS-L516R was still active and exhibited ∼36-fold greater resistance to the bleaching herbicide norflurazon than the unaltered enzyme. Thus, the modified PDS gene could be a useful tool for genetic engineering of carotenoid biosynthesis in C. zofingiensis and perhaps also in other algae. © 2008 Phycological Society of America.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPYen_HK
dc.relation.ispartofJournal of Phycologyen_HK
dc.subjectAstaxanthinen_HK
dc.subjectChlorella zofingiensisen_HK
dc.subjectHerbicideen_HK
dc.subjectPhytoene desaturaseen_HK
dc.subjectPoint mutationen_HK
dc.titleIsolation and characterization of the phytoene desaturase gene as a potential selective marker for genetic engineering of the astaxanthin-producing green alga Chlorella zofingiensis (Chlorophyta)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3646&volume=44&spage=684&epage=690&date=2008&atitle=Isolation+and+characterization+of+the+phytoene+desaturase+gene+as+a+potential+selective+marker+for+genetic+engineering+of+the+astaxanthin-producing+green+alga+Chlorella+zofingiensis+(Chlorophyta).en_HK
dc.identifier.emailHuang, J: huangjc@hku.hken_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityHuang, J=rp00710en_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1529-8817.2008.00511.xen_HK
dc.identifier.scopuseid_2-s2.0-44349110067en_HK
dc.identifier.hkuros147715en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-44349110067&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume44en_HK
dc.identifier.issue3en_HK
dc.identifier.spage684en_HK
dc.identifier.epage690en_HK
dc.identifier.isiWOS:000256123700014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHuang, J=7408108735en_HK
dc.identifier.scopusauthoridLiu, J=36064082300en_HK
dc.identifier.scopusauthoridLi, Y=53464856200en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.citeulike2835151-

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