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Article: Molecular cloning and characterization of chicken prostaglandin E receptor subtypes 2 and 4 (EP2 and EP4)

TitleMolecular cloning and characterization of chicken prostaglandin E receptor subtypes 2 and 4 (EP2 and EP4)
Authors
KeywordscAMP-PKA signaling pathway
Chicken
EP2
EP4
Prostaglandin E2
Issue Date2008
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcen
Citation
General And Comparative Endocrinology, 2008, v. 157 n. 2, p. 99-106 How to Cite?
AbstractProstaglandin E2 (PGE2) is an important chemical mediator responsible for regulation of many vital physiological processes. Four receptor subtypes have been identified to mediate its biological actions. Among these subtypes, prostaglandin E receptor subtypes 2 and 4 (EP2 and EP4), both coupled to cAMP-protein kinase A (cAMP-PKA) signaling pathway, are proposed to play crucial roles under both physiological and pathological conditions. Though both receptors were extensively studied in mammals, little is known about their functionality and expression in non-mammalian species including chicken. In present study, the full-length cDNAs for chicken EP2 and EP4 receptors were first cloned from adult chicken ovary and testis, respectively. Chicken EP2 is 356 amino acids in length and shows high amino acid identity to that of human (61%), mouse (63%), and rat (61%). On the other hand, the full-length cDNA of EP4 gene encodes a precursor of 475 amino acids with a high degree of amino acid identity to that of mammals, including human (87%), mouse (86%), rat (84%), dog (85%), and cattle (83%), and a comparatively lower sequence identity to zebrafish (52%). RT-PCR assays revealed that EP2 mRNA was expressed in all tissues examined including the oviduct, while EP4 expression was detected only in a few tissues. Using the pGL3-CRE-luciferase reporter system, we also demonstrated that PGE2 could induce luciferase activity in DF-1 cells expressing EP2 and EP4 in dose-dependent manners (EC50: <1 nM), confirming that both receptors could be activated by PGE2 and functionally coupled to the cAMP-PKA signaling pathway. Together, our study establishes a molecular basis to understand the physiological roles of PGE2 in target tissues of chicken. © 2008 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/89316
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.245
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKwok, AHYen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorWang, CYen_HK
dc.contributor.authorLeung, FCen_HK
dc.date.accessioned2010-09-06T09:55:19Z-
dc.date.available2010-09-06T09:55:19Z-
dc.date.issued2008en_HK
dc.identifier.citationGeneral And Comparative Endocrinology, 2008, v. 157 n. 2, p. 99-106en_HK
dc.identifier.issn0016-6480en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89316-
dc.description.abstractProstaglandin E2 (PGE2) is an important chemical mediator responsible for regulation of many vital physiological processes. Four receptor subtypes have been identified to mediate its biological actions. Among these subtypes, prostaglandin E receptor subtypes 2 and 4 (EP2 and EP4), both coupled to cAMP-protein kinase A (cAMP-PKA) signaling pathway, are proposed to play crucial roles under both physiological and pathological conditions. Though both receptors were extensively studied in mammals, little is known about their functionality and expression in non-mammalian species including chicken. In present study, the full-length cDNAs for chicken EP2 and EP4 receptors were first cloned from adult chicken ovary and testis, respectively. Chicken EP2 is 356 amino acids in length and shows high amino acid identity to that of human (61%), mouse (63%), and rat (61%). On the other hand, the full-length cDNA of EP4 gene encodes a precursor of 475 amino acids with a high degree of amino acid identity to that of mammals, including human (87%), mouse (86%), rat (84%), dog (85%), and cattle (83%), and a comparatively lower sequence identity to zebrafish (52%). RT-PCR assays revealed that EP2 mRNA was expressed in all tissues examined including the oviduct, while EP4 expression was detected only in a few tissues. Using the pGL3-CRE-luciferase reporter system, we also demonstrated that PGE2 could induce luciferase activity in DF-1 cells expressing EP2 and EP4 in dose-dependent manners (EC50: <1 nM), confirming that both receptors could be activated by PGE2 and functionally coupled to the cAMP-PKA signaling pathway. Together, our study establishes a molecular basis to understand the physiological roles of PGE2 in target tissues of chicken. © 2008 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcenen_HK
dc.relation.ispartofGeneral and Comparative Endocrinologyen_HK
dc.subjectcAMP-PKA signaling pathwayen_HK
dc.subjectChickenen_HK
dc.subjectEP2en_HK
dc.subjectEP4en_HK
dc.subjectProstaglandin E2en_HK
dc.titleMolecular cloning and characterization of chicken prostaglandin E receptor subtypes 2 and 4 (EP2 and EP4)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-6480&volume=157&spage=99&epage=106&date=2008&atitle=Molecular+cloning+and+characterization+of+chicken+prostaglandin+E+receptor+subtypes+2+and+4+(EP2+and+EP4)en_HK
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityWang, Y=rp00801en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ygcen.2008.04.001en_HK
dc.identifier.pmid18486942-
dc.identifier.scopuseid_2-s2.0-44749092276en_HK
dc.identifier.hkuros146218en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-44749092276&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume157en_HK
dc.identifier.issue2en_HK
dc.identifier.spage99en_HK
dc.identifier.epage106en_HK
dc.identifier.isiWOS:000257087000002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKwok, AHY=27168105100en_HK
dc.identifier.scopusauthoridWang, Y=36062525200en_HK
dc.identifier.scopusauthoridWang, CY=16065173000en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK

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