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Article: Biochemical and molecular characterization of PvPAP3, a novel purple acid phosphatase isolated from common bean enhancing extracellular ATP utilization

TitleBiochemical and molecular characterization of PvPAP3, a novel purple acid phosphatase isolated from common bean enhancing extracellular ATP utilization
Authors
Issue Date2010
PublisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org
Citation
Plant Physiology, 2010, v. 152 n. 2, p. 854-865 How to Cite?
AbstractPurple acid phosphatases (PAPs) play diverse physiological roles in plants. In this study, we purified a novel PAP, PvPAP3, from the roots of common bean (Phaseolus vulgaris) grown under phosphate (Pi) starvation. PvPAP3 was identified as a 34-kD monomer acting on the specific substrate, ATP, with a broad pH range and a high heat stability. The activity of PvPAP3 was insensitive to tartrate, indicating that PvPAP3 is a PAP-like protein. Amino acid sequence alignment and phylogenetic analysis suggest that PvPAP3 belongs to the group of plant PAPs with low molecular mass. Transient expression of 35S:PvPAP3-green fluorescent protein in onion (Allium cepa) epidermal cells verified that it might anchor on plasma membrane and be secreted into apoplast. Pi starvation led to induction of PvPAP3 expression in both leaves and roots of common bean, and expression of PvPAP3 was strictly dependent on phosphorus (P) availability and duration of Pi starvation. Furthermore, induction of PvPAP3 expression was more rapid and higher in a P-efficient genotype, G19833, than in a P-inefficient genotype, DOR364, suggesting possible roles of PvPAP3 in P efficiency in bean. In vivo analysis using a transgenic hairy root system of common bean showed that both growth and P uptake of bean hairy roots from the PvPAP3 overexpression transgenic lines were significantly enhanced when ATP was supplied as the sole external P source. Taken together, our results suggest that PvPAP3 is a novel PAP that might function in the adaptation of common bean to P deficiency, possibly through enhancing utilization of extracellular ATP as a P source. © 2009 American Society of Plant Biologists.
Persistent Identifierhttp://hdl.handle.net/10722/89313
ISSN
2015 Impact Factor: 6.28
2015 SCImago Journal Rankings: 3.642
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30890131
National Program on the Development of Basic Research of China2005CB120902
Hong Kong Research Grants Council EarmarkedCUHK4434/04M
Hong Kong UniversityAoE-B-07/09
Funding Information:

This work was supported by the National Natural Science Foundation of China (grant no. 30890131) and the National Program on the Development of Basic Research of China (grant no. 2005CB120902) to X.Y. and H.L., and by the Hong Kong Research Grants Council Earmarked Grant (grant no. CUHK4434/04M) and the Hong Kong University Grants Committee Areas of Excellence Plant and Agricultural Biotechnology Project (grant no. AoE-B-07/09) to H.-M. L.

References

 

DC FieldValueLanguage
dc.contributor.authorLiang, Cen_HK
dc.contributor.authorTian, Jen_HK
dc.contributor.authorLam, HMen_HK
dc.contributor.authorLim, BLen_HK
dc.contributor.authorYan, Xen_HK
dc.contributor.authorLiao, Hen_HK
dc.date.accessioned2010-09-06T09:55:17Z-
dc.date.available2010-09-06T09:55:17Z-
dc.date.issued2010en_HK
dc.identifier.citationPlant Physiology, 2010, v. 152 n. 2, p. 854-865en_HK
dc.identifier.issn0032-0889en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89313-
dc.description.abstractPurple acid phosphatases (PAPs) play diverse physiological roles in plants. In this study, we purified a novel PAP, PvPAP3, from the roots of common bean (Phaseolus vulgaris) grown under phosphate (Pi) starvation. PvPAP3 was identified as a 34-kD monomer acting on the specific substrate, ATP, with a broad pH range and a high heat stability. The activity of PvPAP3 was insensitive to tartrate, indicating that PvPAP3 is a PAP-like protein. Amino acid sequence alignment and phylogenetic analysis suggest that PvPAP3 belongs to the group of plant PAPs with low molecular mass. Transient expression of 35S:PvPAP3-green fluorescent protein in onion (Allium cepa) epidermal cells verified that it might anchor on plasma membrane and be secreted into apoplast. Pi starvation led to induction of PvPAP3 expression in both leaves and roots of common bean, and expression of PvPAP3 was strictly dependent on phosphorus (P) availability and duration of Pi starvation. Furthermore, induction of PvPAP3 expression was more rapid and higher in a P-efficient genotype, G19833, than in a P-inefficient genotype, DOR364, suggesting possible roles of PvPAP3 in P efficiency in bean. In vivo analysis using a transgenic hairy root system of common bean showed that both growth and P uptake of bean hairy roots from the PvPAP3 overexpression transgenic lines were significantly enhanced when ATP was supplied as the sole external P source. Taken together, our results suggest that PvPAP3 is a novel PAP that might function in the adaptation of common bean to P deficiency, possibly through enhancing utilization of extracellular ATP as a P source. © 2009 American Society of Plant Biologists.en_HK
dc.languageengen_HK
dc.publisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.orgen_HK
dc.relation.ispartofPlant Physiologyen_HK
dc.subject.meshAcid Phosphatase - genetics - metabolism-
dc.subject.meshAdenosine Triphosphate - metabolism-
dc.subject.meshGlycoproteins - genetics - metabolism-
dc.subject.meshPhaseolus - enzymology - genetics-
dc.subject.meshPlant Proteins - genetics - metabolism-
dc.titleBiochemical and molecular characterization of PvPAP3, a novel purple acid phosphatase isolated from common bean enhancing extracellular ATP utilizationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0032-0889&volume=152&issue=2&spage=854–865&epage=&date=2010&atitle=Biochemical+and+Molecular+Characterization+of+PvPAP3,+a+Novel+Purple+Acid+Phosphatase+Isolated+from+Common+Bean+Enhancing+Extracellular+ATP+Utilizationen_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1104/pp.109.147918en_HK
dc.identifier.pmid19955264-
dc.identifier.pmcidPMC2815866-
dc.identifier.scopuseid_2-s2.0-75949123248en_HK
dc.identifier.hkuros168304en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-75949123248&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume152en_HK
dc.identifier.issue2en_HK
dc.identifier.spage854en_HK
dc.identifier.epage865en_HK
dc.identifier.isiWOS:000274246600039-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiang, C=35783204500en_HK
dc.identifier.scopusauthoridTian, J=55111185900en_HK
dc.identifier.scopusauthoridLam, HM=7202775017en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK
dc.identifier.scopusauthoridYan, X=7403596595en_HK
dc.identifier.scopusauthoridLiao, H=55121516700en_HK

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