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Article: Overexpression of membrane-associated acyl-CoA-binding protein ACBP1 enhances lead tolerance in Arabidopsis

TitleOverexpression of membrane-associated acyl-CoA-binding protein ACBP1 enhances lead tolerance in Arabidopsis
Authors
KeywordsACBP
Heavy metal
Pb(II) accumulation
Phytoremediation
Plasma membrane
Issue Date2008
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412
Citation
Plant Journal, 2008, v. 54 n. 1, p. 141-151 How to Cite?
AbstractIn Arabidopsis thaliana, a family of six genes encodes acyl-CoA-binding proteins (ACBPs) that show conservation at the acyl-CoA-binding domain. They are the membrane-associated ACBP1 and ACBP2, extracellularly targeted ACBP3, kelch-motif-containing ACBP4 and ACBP5, and 10-kDa ACBP6. The acyl-CoA domain in each of ACBP1 to ACBP6 binds long-chain acyl-CoA esters in vitro, suggestive of possible roles in plant lipid metabolism. We addressed here the use of Arabidopsis ACBPs in conferring lead [Pb(II)] tolerance in transgenic plants because the 10-kDa human ACBP has been identified as a molecular target for Pb(II) in vivo. We investigated the effect of Pb(II) stress on the expression of genes encoding Arabidopsis ACBP1, ACBP2 and ACBP6. We showed that the expression of ACBP1 and ACBP2, but not ACBP6, in root is induced by Pb(II) nitrate treatment. In vitro Pb(II)-binding assays indicated that ACBP1 binds Pb(II) comparatively better, and ACBP1 was therefore selected for further investigations. When grown on Pb(II)-containing medium, transgenic Arabidopsis lines overexpressing ACBP1 were more tolerant to Pb(II)-induced stress than the wild type. Accumulation of Pb(II) in shoots of the ACBP1-overepxressing plants was significantly higher than wild type. The acbp1 mutant showed enhanced sensitivity to Pb(II) when germinated and grown in the presence of Pb(II) nitrate and tolerance was restored upon complementation using an ACBP1 cDNA. Our results suggest that ACBP1 is involved in mediating Pb(II) tolerance in Arabidopsis with accumulation of Pb(II) in shoots. Such observations of Pb(II) accumulation, rather than Pb(II) extrusion, in the ACBP1-overexpressing plants implicate possible use of ACBP1 in Pb(II) phytoremediation. © 2008 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/89299
ISSN
2015 Impact Factor: 5.468
2015 SCImago Journal Rankings: 3.457
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXiao, Sen_HK
dc.contributor.authorGao, Wen_HK
dc.contributor.authorChen, QFen_HK
dc.contributor.authorRamalingam, Sen_HK
dc.contributor.authorChye, MLen_HK
dc.date.accessioned2010-09-06T09:55:06Z-
dc.date.available2010-09-06T09:55:06Z-
dc.date.issued2008en_HK
dc.identifier.citationPlant Journal, 2008, v. 54 n. 1, p. 141-151en_HK
dc.identifier.issn0960-7412en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89299-
dc.description.abstractIn Arabidopsis thaliana, a family of six genes encodes acyl-CoA-binding proteins (ACBPs) that show conservation at the acyl-CoA-binding domain. They are the membrane-associated ACBP1 and ACBP2, extracellularly targeted ACBP3, kelch-motif-containing ACBP4 and ACBP5, and 10-kDa ACBP6. The acyl-CoA domain in each of ACBP1 to ACBP6 binds long-chain acyl-CoA esters in vitro, suggestive of possible roles in plant lipid metabolism. We addressed here the use of Arabidopsis ACBPs in conferring lead [Pb(II)] tolerance in transgenic plants because the 10-kDa human ACBP has been identified as a molecular target for Pb(II) in vivo. We investigated the effect of Pb(II) stress on the expression of genes encoding Arabidopsis ACBP1, ACBP2 and ACBP6. We showed that the expression of ACBP1 and ACBP2, but not ACBP6, in root is induced by Pb(II) nitrate treatment. In vitro Pb(II)-binding assays indicated that ACBP1 binds Pb(II) comparatively better, and ACBP1 was therefore selected for further investigations. When grown on Pb(II)-containing medium, transgenic Arabidopsis lines overexpressing ACBP1 were more tolerant to Pb(II)-induced stress than the wild type. Accumulation of Pb(II) in shoots of the ACBP1-overepxressing plants was significantly higher than wild type. The acbp1 mutant showed enhanced sensitivity to Pb(II) when germinated and grown in the presence of Pb(II) nitrate and tolerance was restored upon complementation using an ACBP1 cDNA. Our results suggest that ACBP1 is involved in mediating Pb(II) tolerance in Arabidopsis with accumulation of Pb(II) in shoots. Such observations of Pb(II) accumulation, rather than Pb(II) extrusion, in the ACBP1-overexpressing plants implicate possible use of ACBP1 in Pb(II) phytoremediation. © 2008 The Authors.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412en_HK
dc.relation.ispartofPlant Journalen_HK
dc.rightsThe Plant Journal. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectACBPen_HK
dc.subjectHeavy metalen_HK
dc.subjectPb(II) accumulationen_HK
dc.subjectPhytoremediationen_HK
dc.subjectPlasma membraneen_HK
dc.titleOverexpression of membrane-associated acyl-CoA-binding protein ACBP1 enhances lead tolerance in Arabidopsisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0960-7412&volume=54&spage=141&epage=151&date=2008&atitle=Overexpression+of+membrane-associated+acyl-CoA-binding+protein+ACBP1+enhances+lead+tolerance+in+Arabidopsisen_HK
dc.identifier.emailXiao, S: xiaoshi@graduate.hku.hken_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityXiao, S=rp00817en_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1365-313X.2008.03402.xen_HK
dc.identifier.pmid18182029-
dc.identifier.scopuseid_2-s2.0-40949119009en_HK
dc.identifier.hkuros142110en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40949119009&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue1en_HK
dc.identifier.spage141en_HK
dc.identifier.epage151en_HK
dc.identifier.isiWOS:000254023600012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridXiao, S=7402022635en_HK
dc.identifier.scopusauthoridGao, W=36045713300en_HK
dc.identifier.scopusauthoridChen, QF=7406335399en_HK
dc.identifier.scopusauthoridRamalingam, S=8709830400en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK
dc.identifier.citeulike2563014-

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