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Article: Differential role of gonadotropin-releasing hormone on human ovarian epithelial cancer cell invasion

TitleDifferential role of gonadotropin-releasing hormone on human ovarian epithelial cancer cell invasion
Authors
KeywordsGnRH
Invasion
Matrix metalloproteinases
Ovarian cancer
PI3K
Issue Date2007
PublisherHumana Press, Inc.
Citation
Endocrine, 2007, v. 31 n. 3, p. 311-320 How to Cite?
AbstractOvarian cancer is the most lethal of all gynecological cancers. Most deaths from ovarian cancer are due to widespread intraperitoneal metastases and malignant ascites. However, mechanisms of invasion in ovarian cancer remain poorly understood. In this study, we examined the effects of gonadotropin-releasing hormone (GnRH)-I (the classical mammalian GnRH), GnRH-II (a second form of GnRH), and GnRH receptor on invasion using two human ovarian carcinoma cell lines, OVCAR-3 and SKOV-3. Here we demonstrated that in OVCAR-3, GnRH-I and GnRH-II promoted cell invasion, whereas in SKOV-3, GnRH-I and GnRH-II inhibited cell invasion. Transfection of small interfering RNA to abrogate the gene expression of GnRH receptor reversed GnRH-I and GnRH-II-mediated invasion activities, suggesting that the same receptor, type I GnRH receptor, is essential for the effects of GnRH-I and GnRH-II in both OVCAR-3 and SKOV-3. Treatment of SKOV-3 cells with GnRH-I or GnRH-II resulted in a decrease in matrix metalloproteinase 2 but an increase in tissue inhibitor of metalloproteinase 2 secretions. In addition, we found that GnRH-I and GnRH-II interfered with activation of the phosphatidylinositol-3-kinase/AKT pathway that is well documented to stimulate proteolysis and invasion of ovarian cancer cells. Taken together, these observations suggest that GnRH-I and GnRH-II play key regulatory roles in ovarian tumor cell invasion and extracellular matrix degradation. © Humana Press Inc. 2007.
Persistent Identifierhttp://hdl.handle.net/10722/89294
ISSN
2015 Impact Factor: 3.279
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, CLen_HK
dc.contributor.authorCheung, LWTen_HK
dc.contributor.authorLau, MTen_HK
dc.contributor.authorChoi, JHen_HK
dc.contributor.authorAuersperg, Nen_HK
dc.contributor.authorWang, HSen_HK
dc.contributor.authorWong, ASTen_HK
dc.contributor.authorLeung, PCKen_HK
dc.date.accessioned2010-09-06T09:55:02Z-
dc.date.available2010-09-06T09:55:02Z-
dc.date.issued2007en_HK
dc.identifier.citationEndocrine, 2007, v. 31 n. 3, p. 311-320en_HK
dc.identifier.issn1355-008Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/89294-
dc.description.abstractOvarian cancer is the most lethal of all gynecological cancers. Most deaths from ovarian cancer are due to widespread intraperitoneal metastases and malignant ascites. However, mechanisms of invasion in ovarian cancer remain poorly understood. In this study, we examined the effects of gonadotropin-releasing hormone (GnRH)-I (the classical mammalian GnRH), GnRH-II (a second form of GnRH), and GnRH receptor on invasion using two human ovarian carcinoma cell lines, OVCAR-3 and SKOV-3. Here we demonstrated that in OVCAR-3, GnRH-I and GnRH-II promoted cell invasion, whereas in SKOV-3, GnRH-I and GnRH-II inhibited cell invasion. Transfection of small interfering RNA to abrogate the gene expression of GnRH receptor reversed GnRH-I and GnRH-II-mediated invasion activities, suggesting that the same receptor, type I GnRH receptor, is essential for the effects of GnRH-I and GnRH-II in both OVCAR-3 and SKOV-3. Treatment of SKOV-3 cells with GnRH-I or GnRH-II resulted in a decrease in matrix metalloproteinase 2 but an increase in tissue inhibitor of metalloproteinase 2 secretions. In addition, we found that GnRH-I and GnRH-II interfered with activation of the phosphatidylinositol-3-kinase/AKT pathway that is well documented to stimulate proteolysis and invasion of ovarian cancer cells. Taken together, these observations suggest that GnRH-I and GnRH-II play key regulatory roles in ovarian tumor cell invasion and extracellular matrix degradation. © Humana Press Inc. 2007.en_HK
dc.languageengen_HK
dc.publisherHumana Press, Inc.en_HK
dc.relation.ispartofEndocrineen_HK
dc.subjectGnRHen_HK
dc.subjectInvasionen_HK
dc.subjectMatrix metalloproteinasesen_HK
dc.subjectOvarian canceren_HK
dc.subjectPI3Ken_HK
dc.titleDifferential role of gonadotropin-releasing hormone on human ovarian epithelial cancer cell invasionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1355-008X&volume=31&spage=311&epage=320&date=2007&atitle=Differential+role+of+gonadotropin-releasing+hormone+on+human+ovarian+epithelial+cancer+cell+invasionen_HK
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_HK
dc.identifier.authorityWong, AST=rp00805en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s12020-007-0041-8en_HK
dc.identifier.pmid17906381-
dc.identifier.scopuseid_2-s2.0-35348864325en_HK
dc.identifier.hkuros130652en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-35348864325&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume31en_HK
dc.identifier.issue3en_HK
dc.identifier.spage311en_HK
dc.identifier.epage320en_HK
dc.identifier.isiWOS:000249871700014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChen, CL=36107678700en_HK
dc.identifier.scopusauthoridCheung, LWT=14119560800en_HK
dc.identifier.scopusauthoridLau, MT=35082179700en_HK
dc.identifier.scopusauthoridChoi, JH=7501395645en_HK
dc.identifier.scopusauthoridAuersperg, N=7006582556en_HK
dc.identifier.scopusauthoridWang, HS=8663433900en_HK
dc.identifier.scopusauthoridWong, AST=23987963300en_HK
dc.identifier.scopusauthoridLeung, PCK=55085135300en_HK

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