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Article: A novel prolactin-like protein (PRL-L) gene in chickens and zebrafish: Cloning and characterization of its tissue expression

TitleA novel prolactin-like protein (PRL-L) gene in chickens and zebrafish: Cloning and characterization of its tissue expression
Authors
KeywordsChicken
Pituitary
Prolactin
Prolactin-like protein
Zebrafish
Issue Date2010
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcen
Citation
General And Comparative Endocrinology, 2010, v. 166 n. 1, p. 200-210 How to Cite?
AbstractIn this study, a full-length cDNA encoding a prolactin-like protein (PRL-L) was cloned from chicken brain tissues using RT-PCR. This putative PRL-L precursor has 225 amino acids in length and shares 30-35% amino acid sequence identity with prolactin (PRL) of chicken, zebrafish, Xenopus, rat and human. Using RT-PCR, the mRNA expression of PRL-L in chicken tissues was further examined. Unlike the predominant expression of PRL in pituitary, PRL-L was found to be widely expressed in adult chicken extra-pituitary tissues with only minimal expression detected in pituitary. In day-7 chicken embryos, the expression of PRL-L, but not PRL, was also detected in all extra-pituitary tissues examined. In line with this finding, the 5′-flanking region of chicken PRL-L (cPRL-L) gene, but not PRL gene, displayed a strong promoter activity in cultured DF-1 cell (a chicken embryo fibroblast cell line), suggesting that the basal expression of PRL-L gene is controlled by a transcriptional regulatory mechanism different from that of PRL gene. As the same findings in chickens, PRL-like protein(s), which share high amino acid sequence (42-86%) identity with chicken PRL-L, was identified in several non-mammalian vertebrate species including zebra finch, tiger puffer, green puffer and zebrafish. RT-PCR assay demonstrated that zebrafish PRL-L, similar to chicken PRL-L, is expressed in extra-pituitary tissues including brain, gill, muscle, ovary and testis. Taken together, these findings strongly suggest that a novel PRL-like protein exists in some non-mammalian vertebrates and may play an important role in target tissues, such as extra-pituitary tissues of chickens and zebrafish. © 2009 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/89290
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.245
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council of the Hong Kong GovernmentHKU7345/03M
National Natural Science Foundation of China30771171
30871338
National High Technology Research and Development Program of China2007AA10Z163
Funding Information:

We would like to give special thanks to the two anonymous reviewers for their constructive suggestions for the publication of this manuscript. This work was supported by grants from the Research Grant Council of the Hong Kong Government (HKU7345/03M), the National Natural Science Foundation of China (30771171, 30871338), and the National High Technology Research and Development Program of China (2007AA10Z163).

References

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorYan Kwok, AHen_HK
dc.contributor.authorGe, Wen_HK
dc.contributor.authorLeung, FCen_HK
dc.date.accessioned2010-09-06T09:54:59Z-
dc.date.available2010-09-06T09:54:59Z-
dc.date.issued2010en_HK
dc.identifier.citationGeneral And Comparative Endocrinology, 2010, v. 166 n. 1, p. 200-210en_HK
dc.identifier.issn0016-6480en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89290-
dc.description.abstractIn this study, a full-length cDNA encoding a prolactin-like protein (PRL-L) was cloned from chicken brain tissues using RT-PCR. This putative PRL-L precursor has 225 amino acids in length and shares 30-35% amino acid sequence identity with prolactin (PRL) of chicken, zebrafish, Xenopus, rat and human. Using RT-PCR, the mRNA expression of PRL-L in chicken tissues was further examined. Unlike the predominant expression of PRL in pituitary, PRL-L was found to be widely expressed in adult chicken extra-pituitary tissues with only minimal expression detected in pituitary. In day-7 chicken embryos, the expression of PRL-L, but not PRL, was also detected in all extra-pituitary tissues examined. In line with this finding, the 5′-flanking region of chicken PRL-L (cPRL-L) gene, but not PRL gene, displayed a strong promoter activity in cultured DF-1 cell (a chicken embryo fibroblast cell line), suggesting that the basal expression of PRL-L gene is controlled by a transcriptional regulatory mechanism different from that of PRL gene. As the same findings in chickens, PRL-like protein(s), which share high amino acid sequence (42-86%) identity with chicken PRL-L, was identified in several non-mammalian vertebrate species including zebra finch, tiger puffer, green puffer and zebrafish. RT-PCR assay demonstrated that zebrafish PRL-L, similar to chicken PRL-L, is expressed in extra-pituitary tissues including brain, gill, muscle, ovary and testis. Taken together, these findings strongly suggest that a novel PRL-like protein exists in some non-mammalian vertebrates and may play an important role in target tissues, such as extra-pituitary tissues of chickens and zebrafish. © 2009 Elsevier Inc.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcenen_HK
dc.relation.ispartofGeneral and Comparative Endocrinologyen_HK
dc.subjectChickenen_HK
dc.subjectPituitaryen_HK
dc.subjectProlactinen_HK
dc.subjectProlactin-like proteinen_HK
dc.subjectZebrafishen_HK
dc.subject.meshAmino Acid Sequence-
dc.subject.meshChickens-
dc.subject.meshChromosome Mapping-
dc.subject.meshProlactin - genetics-
dc.subject.meshZebrafish-
dc.titleA novel prolactin-like protein (PRL-L) gene in chickens and zebrafish: Cloning and characterization of its tissue expressionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-6480&volume=166&issue=1&spage=200&epage=210&date=2010&atitle=A+novel+prolactin-like+protein+(PRL-L)+gene+in+chickens+and+zebrafish:+cloning+and+characterization+of+its+tissue+expressionen_HK
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityWang, Y=rp00801en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ygcen.2009.10.007en_HK
dc.identifier.pmid19854191-
dc.identifier.scopuseid_2-s2.0-77349117507en_HK
dc.identifier.hkuros168611en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77349117507&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume166en_HK
dc.identifier.issue1en_HK
dc.identifier.spage200en_HK
dc.identifier.epage210en_HK
dc.identifier.isiWOS:000274673500024-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, Y=36062525200en_HK
dc.identifier.scopusauthoridLi, J=27172363500en_HK
dc.identifier.scopusauthoridYan Kwok, AH=36437957200en_HK
dc.identifier.scopusauthoridGe, W=22979306700en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK
dc.identifier.citeulike6048541-

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