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Article: Lactobacillus rhamnosus strain GG restores alkaline phosphatase activity in differentiating Caco-2 cells dosed with the potent mycotoxin deoxynivalenol

TitleLactobacillus rhamnosus strain GG restores alkaline phosphatase activity in differentiating Caco-2 cells dosed with the potent mycotoxin deoxynivalenol
Authors
KeywordsCaco-2
Deoxynivalenol
Intestine
Lactobacillus
Probiotic
Issue Date2008
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/foodchemtox
Citation
Food And Chemical Toxicology, 2008, v. 46 n. 6, p. 2118-2123 How to Cite?
AbstractDeoxynivalenol (DON) contamination of cereal crops occurs frequently, and may cause acute exposure at high levels or chronic more moderate exposure. DON has proven toxicity including restriction of enterocyte differentiation, which may play a part in DON induced gastroenteritis. The probiotic bacteria Lactobacillus rhamnosus strain GG (GG) can bind DON, and therefore potentially restrict bioavailability of this toxin. Binding efficacy is not significantly altered by heat treatment, and therefore this in vitro study evaluated whether heat inactivated GG could restore the differentiation process in Caco-2 cells, using alkaline phosphatase (ALP) activity as a marker of differentiation. DON (200 ng/mL) caused a significant (p < 0.001) 36% reduction in ALP activity (1598 ± 137 U/mg protein) compared to untreated cells (2502 ± 80 U/mg). A dose dependant restoration of ALP activity was observed where DON treated cells were co-incubated with heat inactivated GG (1719 ± 84; 2007 ± 142; 2272 ± 160 U/mg for GG at 1 × 104 (p > 0.9), 1 × 107 (p < 0.001), and 1 × 1010 CFU/mL (p < 0.001), respectively). Co-incubation of the non-binding strain, LC-705 (1 × 1010 CFU/mL), with DON did not significantly restore the ALP (1841 ± 97 U/mg, p < 0.077) compared to DON only treated cells. When viable GG were co-incubated with DON a similar restoration of ALP activity was observed as seen for heat inactivated GG. These combined data suggest that the major effect of GG on restoring ALP activity, and therefore Caco-2 cell differentiation, was due to specific binding of DON, with possibly a more minor role of non-specific bacterial interference. © 2008 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/89281
ISSN
2015 Impact Factor: 3.584
2015 SCImago Journal Rankings: 1.190
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTurner, PCen_HK
dc.contributor.authorWu, QKen_HK
dc.contributor.authorPiekkola, Sen_HK
dc.contributor.authorGratz, Sen_HK
dc.contributor.authorMykkänen, Hen_HK
dc.contributor.authorElNezami, Hen_HK
dc.date.accessioned2010-09-06T09:54:52Z-
dc.date.available2010-09-06T09:54:52Z-
dc.date.issued2008en_HK
dc.identifier.citationFood And Chemical Toxicology, 2008, v. 46 n. 6, p. 2118-2123en_HK
dc.identifier.issn0278-6915en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89281-
dc.description.abstractDeoxynivalenol (DON) contamination of cereal crops occurs frequently, and may cause acute exposure at high levels or chronic more moderate exposure. DON has proven toxicity including restriction of enterocyte differentiation, which may play a part in DON induced gastroenteritis. The probiotic bacteria Lactobacillus rhamnosus strain GG (GG) can bind DON, and therefore potentially restrict bioavailability of this toxin. Binding efficacy is not significantly altered by heat treatment, and therefore this in vitro study evaluated whether heat inactivated GG could restore the differentiation process in Caco-2 cells, using alkaline phosphatase (ALP) activity as a marker of differentiation. DON (200 ng/mL) caused a significant (p < 0.001) 36% reduction in ALP activity (1598 ± 137 U/mg protein) compared to untreated cells (2502 ± 80 U/mg). A dose dependant restoration of ALP activity was observed where DON treated cells were co-incubated with heat inactivated GG (1719 ± 84; 2007 ± 142; 2272 ± 160 U/mg for GG at 1 × 104 (p > 0.9), 1 × 107 (p < 0.001), and 1 × 1010 CFU/mL (p < 0.001), respectively). Co-incubation of the non-binding strain, LC-705 (1 × 1010 CFU/mL), with DON did not significantly restore the ALP (1841 ± 97 U/mg, p < 0.077) compared to DON only treated cells. When viable GG were co-incubated with DON a similar restoration of ALP activity was observed as seen for heat inactivated GG. These combined data suggest that the major effect of GG on restoring ALP activity, and therefore Caco-2 cell differentiation, was due to specific binding of DON, with possibly a more minor role of non-specific bacterial interference. © 2008 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/foodchemtoxen_HK
dc.relation.ispartofFood and Chemical Toxicologyen_HK
dc.subjectCaco-2en_HK
dc.subjectDeoxynivalenolen_HK
dc.subjectIntestineen_HK
dc.subjectLactobacillusen_HK
dc.subjectProbioticen_HK
dc.titleLactobacillus rhamnosus strain GG restores alkaline phosphatase activity in differentiating Caco-2 cells dosed with the potent mycotoxin deoxynivalenolen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0278-6915&volume=46&spage=2118&epage=23&date=2008&atitle=Lactobacillus+rhamnosus+strain+GG+restores+alkaline+phosphatase+activity+in+differentiating+Caco-2+cells+dosed+with+the+potent+mycotoxin+deoxynivalenol.en_HK
dc.identifier.emailElNezami, H: elnezami@hkucc.hku.hken_HK
dc.identifier.authorityElNezami, H=rp00694en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.fct.2008.02.004en_HK
dc.identifier.pmid18343010-
dc.identifier.scopuseid_2-s2.0-43049181933en_HK
dc.identifier.hkuros151558en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-43049181933&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue6en_HK
dc.identifier.spage2118en_HK
dc.identifier.epage2123en_HK
dc.identifier.isiWOS:000256512200029-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTurner, PC=7402096074en_HK
dc.identifier.scopusauthoridWu, QK=36801000100en_HK
dc.identifier.scopusauthoridPiekkola, S=23973588700en_HK
dc.identifier.scopusauthoridGratz, S=9242677500en_HK
dc.identifier.scopusauthoridMykkänen, H=7003915985en_HK
dc.identifier.scopusauthoridElNezami, H=6603690577en_HK

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