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Article: Identification and characterization of molecular targets of natural products by mass spectrometry

TitleIdentification and characterization of molecular targets of natural products by mass spectrometry
Authors
KeywordsMass spectrometry
Natural products
Proteomics
Target identification
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0277-7037/
Citation
Mass Spectrometry Reviews, 2010, v. 29 n. 1, p. 126-155 How to Cite?
AbstractNatural products, and their derivatives and mimics, have contributed to the development of important therapeutics to combat diseases such as infections and cancers over the past decades. The value of natural products to modern drug discovery is still considerable. However, its development is hampered by a lack of a mechanistic understanding of their molecular action, as opposed to the emerging molecule-targeted therapeutics that are tailored to a specific protein target(s). Recent advances in the mass spectrometry-based proteomic approaches have the potential to offer unprecedented insights into the molecular action of natural products. Chemical proteomics is established as an invaluable tool for the identification of protein targets of natural products. Small-molecule affinity selection combined with mass spectrometry is a successful strategy to "fish"cellular targets from the entire proteome. Mass spectrometry-based profiling of protein expression is also routinely employed to elucidate molecular pathways involved in the therapeutic and possible toxicological responses upon treatment with natural products. In addition, mass spectrometry is increasingly utilized to probe structural aspects of natural products-protein interactions. Limited proteolysis, photoaffinity labeling, and hydrogen/deuterium exchange in conjunction with mass spectrometry are sensitive and high-throughput strategies that provide low-resolution structural information of non-covalent natural product-protein complexes. In this review, we provide an overview on the applications of mass spectrometry-based techniques in the identification and characterization of natural product-protein interactions, and we describe how these applications might revolutionize natural product-based drug discovery. © 2009 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/89270
ISSN
2015 Impact Factor: 9.346
2015 SCImago Journal Rankings: 2.504
ISI Accession Number ID
Funding AgencyGrant Number
HKU Genomics
Proteomics and Bioinformatics Strategic Research Theme
HKU Outstanding YOUng Researcher Award
HKU Outstanding Research Student Supervisor Award
Funding Information:

The authors wish to acknowledge the support of HKU Genomics, Proteomics and Bioinformatics Strategic Research Theme, the HKU Outstanding YOUng Researcher Award and the HKU Outstanding Research Student Supervisor Award.

References

 

DC FieldValueLanguage
dc.contributor.authorCheng, KWen_HK
dc.contributor.authorWong, CCen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorHe, QYen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T09:54:42Z-
dc.date.available2010-09-06T09:54:42Z-
dc.date.issued2010en_HK
dc.identifier.citationMass Spectrometry Reviews, 2010, v. 29 n. 1, p. 126-155en_HK
dc.identifier.issn0277-7037en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89270-
dc.description.abstractNatural products, and their derivatives and mimics, have contributed to the development of important therapeutics to combat diseases such as infections and cancers over the past decades. The value of natural products to modern drug discovery is still considerable. However, its development is hampered by a lack of a mechanistic understanding of their molecular action, as opposed to the emerging molecule-targeted therapeutics that are tailored to a specific protein target(s). Recent advances in the mass spectrometry-based proteomic approaches have the potential to offer unprecedented insights into the molecular action of natural products. Chemical proteomics is established as an invaluable tool for the identification of protein targets of natural products. Small-molecule affinity selection combined with mass spectrometry is a successful strategy to "fish"cellular targets from the entire proteome. Mass spectrometry-based profiling of protein expression is also routinely employed to elucidate molecular pathways involved in the therapeutic and possible toxicological responses upon treatment with natural products. In addition, mass spectrometry is increasingly utilized to probe structural aspects of natural products-protein interactions. Limited proteolysis, photoaffinity labeling, and hydrogen/deuterium exchange in conjunction with mass spectrometry are sensitive and high-throughput strategies that provide low-resolution structural information of non-covalent natural product-protein complexes. In this review, we provide an overview on the applications of mass spectrometry-based techniques in the identification and characterization of natural product-protein interactions, and we describe how these applications might revolutionize natural product-based drug discovery. © 2009 Wiley Periodicals, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0277-7037/en_HK
dc.relation.ispartofMass Spectrometry Reviewsen_HK
dc.rightsMass Spectrometry Reviews. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectMass spectrometryen_HK
dc.subjectNatural productsen_HK
dc.subjectProteomicsen_HK
dc.subjectTarget identificationen_HK
dc.subject.meshBiological Products - analysis - metabolism-
dc.subject.meshDrug Design-
dc.subject.meshMass Spectrometry - methods-
dc.subject.meshProteins - analysis - metabolism-
dc.subject.meshProteomics - methods-
dc.titleIdentification and characterization of molecular targets of natural products by mass spectrometryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0277-7037&volume=29&issue=1&spage=126&epage=155&date=2010&atitle=Identification+and+characterization+of+molecular+targets+of+natural+products+by+mass+spectrometryen_HK
dc.identifier.emailWang, M: mfwang@hku.hken_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityWang, M=rp00800en_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/mas.20235en_HK
dc.identifier.pmid19319922-
dc.identifier.scopuseid_2-s2.0-73849090207en_HK
dc.identifier.hkuros169746en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-73849090207&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue1en_HK
dc.identifier.spage126en_HK
dc.identifier.epage155en_HK
dc.identifier.isiWOS:000273518300006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCheng, KW=12141247000en_HK
dc.identifier.scopusauthoridWong, CC=35333095100en_HK
dc.identifier.scopusauthoridWang, M=7406691844en_HK
dc.identifier.scopusauthoridHe, QY=34770287900en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.citeulike6463468-

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