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Article: Lycium barbarum polysaccharides induce apoptosis in human prostate cancer cells and inhibits prostate cancer growth in a xenograft mouse model of human prostate cancer

TitleLycium barbarum polysaccharides induce apoptosis in human prostate cancer cells and inhibits prostate cancer growth in a xenograft mouse model of human prostate cancer
Authors
KeywordsAntitumor
Apoptosis
Human prostate cancer cells
Lycium barbarum
Polysaccharides
Proliferation
Issue Date2009
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/jmf
Citation
Journal Of Medicinal Food, 2009, v. 12 n. 4, p. 695-703 How to Cite?
AbstractLycium barbarum polysaccharides (LBPs) are important functional constituents in red-colored fruits of L. barbarum (Guo Qi Zi, a well-known traditional Chinese medicinal plant commonly known as Goji berry or wolfberry). The influence of LBP on human prostate cancer cells was systematically investigated in vitro and in vivo. The in vitro effects of LBP on two cell lines (PC-3 and DU-145) were examined by using trypan blue exclusion staining, single-cell gel electrophoresis, flow cytometry, terminal dUTP nick-end labeling assay, and immunohistochemical assay (assessment of Bcl-2 and Bax expression). The in vivo effect of LBP on PC-3 cells was assessed in the nude mouse xenograft tumor model. The in vitro results showed that LBP can dose- and time-dependently inhibit the growth of both PC-3 and DU-145 cells. LBP caused the breakage of DNA strands of PC-3 and DU-145 cells; the tail frequency and tail length were significantly higher than that of control cells. LBP also markedly induced PC-3 and DU-145 cell apoptosis, with the highest apoptosis rates at 41.5% and 35.5%, respectively. The ratio of Bcl-2/Bax protein expression following LBP treatments decreased significantly with a dose-effect relationship, which suggested that LBP can regulate the expression of Bcl-2 and Bax to induce apoptosis of PC-3 and DU-145 cells. The in vivo experimental results indicate that LBP might significantly inhibit PC-3 tumor growth in nude mice. Both the tumor volume and weight of the LBP treatment group were significantly lower than those of the control group. © 2009 Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition.
Persistent Identifierhttp://hdl.handle.net/10722/89246
ISSN
2014 Impact Factor: 1.626
2013 SCImago Journal Rankings: 0.615
ISI Accession Number ID
Funding AgencyGrant Number
Bureau of Science and Technology of Hubei, China
Food Nutrition and Education Fund of DANONE Institute (China)
University of Hong Kong
Funding Information:

This work was supported by a key program funding from the Bureau of Science and Technology of Hubei, China, the Food Nutrition and Education Fund of DANONE Institute (China), and the University of Hong Kong Seed Funding for Basic Research.

References

 

DC FieldValueLanguage
dc.contributor.authorLuo, Qen_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorYan, Jen_HK
dc.contributor.authorZhu, Fen_HK
dc.contributor.authorXu, RJen_HK
dc.contributor.authorCai, YZen_HK
dc.date.accessioned2010-09-06T09:54:24Z-
dc.date.available2010-09-06T09:54:24Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Medicinal Food, 2009, v. 12 n. 4, p. 695-703en_HK
dc.identifier.issn1096-620Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/89246-
dc.description.abstractLycium barbarum polysaccharides (LBPs) are important functional constituents in red-colored fruits of L. barbarum (Guo Qi Zi, a well-known traditional Chinese medicinal plant commonly known as Goji berry or wolfberry). The influence of LBP on human prostate cancer cells was systematically investigated in vitro and in vivo. The in vitro effects of LBP on two cell lines (PC-3 and DU-145) were examined by using trypan blue exclusion staining, single-cell gel electrophoresis, flow cytometry, terminal dUTP nick-end labeling assay, and immunohistochemical assay (assessment of Bcl-2 and Bax expression). The in vivo effect of LBP on PC-3 cells was assessed in the nude mouse xenograft tumor model. The in vitro results showed that LBP can dose- and time-dependently inhibit the growth of both PC-3 and DU-145 cells. LBP caused the breakage of DNA strands of PC-3 and DU-145 cells; the tail frequency and tail length were significantly higher than that of control cells. LBP also markedly induced PC-3 and DU-145 cell apoptosis, with the highest apoptosis rates at 41.5% and 35.5%, respectively. The ratio of Bcl-2/Bax protein expression following LBP treatments decreased significantly with a dose-effect relationship, which suggested that LBP can regulate the expression of Bcl-2 and Bax to induce apoptosis of PC-3 and DU-145 cells. The in vivo experimental results indicate that LBP might significantly inhibit PC-3 tumor growth in nude mice. Both the tumor volume and weight of the LBP treatment group were significantly lower than those of the control group. © 2009 Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition.en_HK
dc.languageengen_HK
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/jmfen_HK
dc.relation.ispartofJournal of Medicinal Fooden_HK
dc.subjectAntitumoren_HK
dc.subjectApoptosisen_HK
dc.subjectHuman prostate cancer cellsen_HK
dc.subjectLycium barbarumen_HK
dc.subjectPolysaccharidesen_HK
dc.subjectProliferationen_HK
dc.titleLycium barbarum polysaccharides induce apoptosis in human prostate cancer cells and inhibits prostate cancer growth in a xenograft mouse model of human prostate canceren_HK
dc.typeArticleen_HK
dc.identifier.emailXu, RJ: xuruojun@hkucc.hku.hken_HK
dc.identifier.emailCai, YZ: yzcai@hkucc.hku.hken_HK
dc.identifier.authorityXu, RJ=rp00820en_HK
dc.identifier.authorityCai, YZ=rp00661en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1089/jmf.2008.1232en_HK
dc.identifier.pmid19735167-
dc.identifier.scopuseid_2-s2.0-70149104250en_HK
dc.identifier.hkuros158397en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70149104250&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue4en_HK
dc.identifier.spage695en_HK
dc.identifier.epage703en_HK
dc.identifier.isiWOS:000269569500001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLuo, Q=35311306700en_HK
dc.identifier.scopusauthoridLi, Z=14019808400en_HK
dc.identifier.scopusauthoridYan, J=7403729064en_HK
dc.identifier.scopusauthoridZhu, F=35306203800en_HK
dc.identifier.scopusauthoridXu, RJ=7402813973en_HK
dc.identifier.scopusauthoridCai, YZ=8684149300en_HK

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