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Article: Lycium barbarum polysaccharides induce apoptosis in human prostate cancer cells and inhibits prostate cancer growth in a xenograft mouse model of human prostate cancer
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TitleLycium barbarum polysaccharides induce apoptosis in human prostate cancer cells and inhibits prostate cancer growth in a xenograft mouse model of human prostate cancer
 
AuthorsLuo, Q2
Li, Z2
Yan, J2
Zhu, F1
Xu, RJ1
Cai, YZ2 1
 
KeywordsAntitumor
Apoptosis
Human prostate cancer cells
Lycium barbarum
Polysaccharides
Proliferation
 
Issue Date2009
 
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/jmf
 
CitationJournal Of Medicinal Food, 2009, v. 12 n. 4, p. 695-703 [How to Cite?]
DOI: http://dx.doi.org/10.1089/jmf.2008.1232
 
AbstractLycium barbarum polysaccharides (LBPs) are important functional constituents in red-colored fruits of L. barbarum (Guo Qi Zi, a well-known traditional Chinese medicinal plant commonly known as Goji berry or wolfberry). The influence of LBP on human prostate cancer cells was systematically investigated in vitro and in vivo. The in vitro effects of LBP on two cell lines (PC-3 and DU-145) were examined by using trypan blue exclusion staining, single-cell gel electrophoresis, flow cytometry, terminal dUTP nick-end labeling assay, and immunohistochemical assay (assessment of Bcl-2 and Bax expression). The in vivo effect of LBP on PC-3 cells was assessed in the nude mouse xenograft tumor model. The in vitro results showed that LBP can dose- and time-dependently inhibit the growth of both PC-3 and DU-145 cells. LBP caused the breakage of DNA strands of PC-3 and DU-145 cells; the tail frequency and tail length were significantly higher than that of control cells. LBP also markedly induced PC-3 and DU-145 cell apoptosis, with the highest apoptosis rates at 41.5% and 35.5%, respectively. The ratio of Bcl-2/Bax protein expression following LBP treatments decreased significantly with a dose-effect relationship, which suggested that LBP can regulate the expression of Bcl-2 and Bax to induce apoptosis of PC-3 and DU-145 cells. The in vivo experimental results indicate that LBP might significantly inhibit PC-3 tumor growth in nude mice. Both the tumor volume and weight of the LBP treatment group were significantly lower than those of the control group. © 2009 Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition.
 
ISSN1096-620X
2013 Impact Factor: 1.699
2013 SCImago Journal Rankings: 0.615
 
DOIhttp://dx.doi.org/10.1089/jmf.2008.1232
 
ISI Accession Number IDWOS:000269569500001
Funding AgencyGrant Number
Bureau of Science and Technology of Hubei, China
Food Nutrition and Education Fund of DANONE Institute (China)
University of Hong Kong
Funding Information:

This work was supported by a key program funding from the Bureau of Science and Technology of Hubei, China, the Food Nutrition and Education Fund of DANONE Institute (China), and the University of Hong Kong Seed Funding for Basic Research.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLuo, Q
 
dc.contributor.authorLi, Z
 
dc.contributor.authorYan, J
 
dc.contributor.authorZhu, F
 
dc.contributor.authorXu, RJ
 
dc.contributor.authorCai, YZ
 
dc.date.accessioned2010-09-06T09:54:24Z
 
dc.date.available2010-09-06T09:54:24Z
 
dc.date.issued2009
 
dc.description.abstractLycium barbarum polysaccharides (LBPs) are important functional constituents in red-colored fruits of L. barbarum (Guo Qi Zi, a well-known traditional Chinese medicinal plant commonly known as Goji berry or wolfberry). The influence of LBP on human prostate cancer cells was systematically investigated in vitro and in vivo. The in vitro effects of LBP on two cell lines (PC-3 and DU-145) were examined by using trypan blue exclusion staining, single-cell gel electrophoresis, flow cytometry, terminal dUTP nick-end labeling assay, and immunohistochemical assay (assessment of Bcl-2 and Bax expression). The in vivo effect of LBP on PC-3 cells was assessed in the nude mouse xenograft tumor model. The in vitro results showed that LBP can dose- and time-dependently inhibit the growth of both PC-3 and DU-145 cells. LBP caused the breakage of DNA strands of PC-3 and DU-145 cells; the tail frequency and tail length were significantly higher than that of control cells. LBP also markedly induced PC-3 and DU-145 cell apoptosis, with the highest apoptosis rates at 41.5% and 35.5%, respectively. The ratio of Bcl-2/Bax protein expression following LBP treatments decreased significantly with a dose-effect relationship, which suggested that LBP can regulate the expression of Bcl-2 and Bax to induce apoptosis of PC-3 and DU-145 cells. The in vivo experimental results indicate that LBP might significantly inhibit PC-3 tumor growth in nude mice. Both the tumor volume and weight of the LBP treatment group were significantly lower than those of the control group. © 2009 Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Medicinal Food, 2009, v. 12 n. 4, p. 695-703 [How to Cite?]
DOI: http://dx.doi.org/10.1089/jmf.2008.1232
 
dc.identifier.doihttp://dx.doi.org/10.1089/jmf.2008.1232
 
dc.identifier.epage703
 
dc.identifier.hkuros158397
 
dc.identifier.isiWOS:000269569500001
Funding AgencyGrant Number
Bureau of Science and Technology of Hubei, China
Food Nutrition and Education Fund of DANONE Institute (China)
University of Hong Kong
Funding Information:

This work was supported by a key program funding from the Bureau of Science and Technology of Hubei, China, the Food Nutrition and Education Fund of DANONE Institute (China), and the University of Hong Kong Seed Funding for Basic Research.

 
dc.identifier.issn1096-620X
2013 Impact Factor: 1.699
2013 SCImago Journal Rankings: 0.615
 
dc.identifier.issue4
 
dc.identifier.pmid19735167
 
dc.identifier.scopuseid_2-s2.0-70149104250
 
dc.identifier.spage695
 
dc.identifier.urihttp://hdl.handle.net/10722/89246
 
dc.identifier.volume12
 
dc.languageeng
 
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/jmf
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Medicinal Food
 
dc.relation.referencesReferences in Scopus
 
dc.subjectAntitumor
 
dc.subjectApoptosis
 
dc.subjectHuman prostate cancer cells
 
dc.subjectLycium barbarum
 
dc.subjectPolysaccharides
 
dc.subjectProliferation
 
dc.titleLycium barbarum polysaccharides induce apoptosis in human prostate cancer cells and inhibits prostate cancer growth in a xenograft mouse model of human prostate cancer
 
dc.typeArticle
 
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<contributor.author>Zhu, F</contributor.author>
<contributor.author>Xu, RJ</contributor.author>
<contributor.author>Cai, YZ</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Wuhan University