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Article: 5′ HS5 of the Human β-globin Locus Control Region is dispensable for the formation of the β-globin active chromatin hub
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Title5′ HS5 of the Human β-globin Locus Control Region is dispensable for the formation of the β-globin active chromatin hub
 
AuthorsChan, PK2
Wai, A1
Philipsen, S1
TanUn, KC2
 
Issue Date2008
 
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
CitationPlos One, 2008, v. 3 n. 5 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0002134
 
AbstractHypersensitive site 5 (5′HS5) of the β-globin Locus Control Region functions as a developmental stage-specific border in erythroid cells. Here, we have analyzed the role of 59HS5 in the three dimensional organization of the β-gene locus using the Chromatin Conformation Capture (3C) technique. The results show that when 5′HS5 is deleted from the locus, both remote and internal regulatory elements are still able to interact with each other in a three-dimensional configuration termed the Active Chromatin Hub. Thus, the absence of 5′HS5 does not have an appreciable effect on the three dimensional organization of the β-globin locus. This rules out models in which 5′HS5 nucleates interactions with remote and/or internal regulatory elements. We also determined the binding of CTCF, the only defined insulator protein in mammalian cells, to 5′HS5 by using chromatin immunoprecipitation (ChIP) assays. We detect low levels of CTCF binding to 5′HS5 in primitive erythroid cells, in which it functions as a border element. Surprisingly, we also observe binding levels of CTCF to 5′HS5 in definitive erythroid cells. Thus, binding of CTCF to 5′HS5 per se does not render it a functional border element. This is consistent with the previous data suggesting that CTCF has dual functionality. © 2008 Chan et al.
 
ISSN1932-6203
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
 
DOIhttp://dx.doi.org/10.1371/journal.pone.0002134
 
PubMed Central IDPMC2358975
 
ISI Accession Number IDWOS:000261642400055
Funding AgencyGrant Number
HKU School of Professional and Continuing Education (HKU SPACE)
Funding Information:

This work was supported by the HKU School of Professional and Continuing Education (HKU SPACE).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChan, PK
 
dc.contributor.authorWai, A
 
dc.contributor.authorPhilipsen, S
 
dc.contributor.authorTanUn, KC
 
dc.date.accessioned2010-09-06T09:54:18Z
 
dc.date.available2010-09-06T09:54:18Z
 
dc.date.issued2008
 
dc.description.abstractHypersensitive site 5 (5′HS5) of the β-globin Locus Control Region functions as a developmental stage-specific border in erythroid cells. Here, we have analyzed the role of 59HS5 in the three dimensional organization of the β-gene locus using the Chromatin Conformation Capture (3C) technique. The results show that when 5′HS5 is deleted from the locus, both remote and internal regulatory elements are still able to interact with each other in a three-dimensional configuration termed the Active Chromatin Hub. Thus, the absence of 5′HS5 does not have an appreciable effect on the three dimensional organization of the β-globin locus. This rules out models in which 5′HS5 nucleates interactions with remote and/or internal regulatory elements. We also determined the binding of CTCF, the only defined insulator protein in mammalian cells, to 5′HS5 by using chromatin immunoprecipitation (ChIP) assays. We detect low levels of CTCF binding to 5′HS5 in primitive erythroid cells, in which it functions as a border element. Surprisingly, we also observe binding levels of CTCF to 5′HS5 in definitive erythroid cells. Thus, binding of CTCF to 5′HS5 per se does not render it a functional border element. This is consistent with the previous data suggesting that CTCF has dual functionality. © 2008 Chan et al.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationPlos One, 2008, v. 3 n. 5 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0002134
 
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0002134
 
dc.identifier.eissn1932-6203
 
dc.identifier.epagee2134
 
dc.identifier.hkuros142401
 
dc.identifier.isiWOS:000261642400055
Funding AgencyGrant Number
HKU School of Professional and Continuing Education (HKU SPACE)
Funding Information:

This work was supported by the HKU School of Professional and Continuing Education (HKU SPACE).

 
dc.identifier.issn1932-6203
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
 
dc.identifier.issue5
 
dc.identifier.pmcidPMC2358975
 
dc.identifier.pmid18461170
 
dc.identifier.scopuseid_2-s2.0-47749114963
 
dc.identifier.spagee2134
 
dc.identifier.urihttp://hdl.handle.net/10722/89238
 
dc.identifier.volume3
 
dc.languageeng
 
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
dc.publisher.placeUnited States
 
dc.relation.ispartofPLoS ONE
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshChromatin - genetics
 
dc.subject.meshDNA-Binding Proteins - genetics
 
dc.subject.meshErythrocytes - physiology
 
dc.subject.meshGlobins - chemistry - genetics
 
dc.subject.meshLocus Control Region - genetics
 
dc.title5′ HS5 of the Human β-globin Locus Control Region is dispensable for the formation of the β-globin active chromatin hub
 
dc.typeArticle
 
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<description.abstract>Hypersensitive site 5 (5&#8242;HS5) of the &#946;-globin Locus Control Region functions as a developmental stage-specific border in erythroid cells. Here, we have analyzed the role of 59HS5 in the three dimensional organization of the &#946;-gene locus using the Chromatin Conformation Capture (3C) technique. The results show that when 5&#8242;HS5 is deleted from the locus, both remote and internal regulatory elements are still able to interact with each other in a three-dimensional configuration termed the Active Chromatin Hub. Thus, the absence of 5&#8242;HS5 does not have an appreciable effect on the three dimensional organization of the &#946;-globin locus. This rules out models in which 5&#8242;HS5 nucleates interactions with remote and/or internal regulatory elements. We also determined the binding of CTCF, the only defined insulator protein in mammalian cells, to 5&#8242;HS5 by using chromatin immunoprecipitation (ChIP) assays. We detect low levels of CTCF binding to 5&#8242;HS5 in primitive erythroid cells, in which it functions as a border element. Surprisingly, we also observe binding levels of CTCF to 5&#8242;HS5 in definitive erythroid cells. Thus, binding of CTCF to 5&#8242;HS5 per se does not render it a functional border element. This is consistent with the previous data suggesting that CTCF has dual functionality. &#169; 2008 Chan et al.</description.abstract>
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Author Affiliations
  1. Erasmus University Medical Center
  2. The University of Hong Kong