Article: 5′ HS5 of the Human β-globin Locus Control Region is dispensable for the formation of the β-globin active chromatin hub
| Title | 5′ HS5 of the Human β-globin Locus Control Region is dispensable for the formation of the β-globin active chromatin hub | ||||
|---|---|---|---|---|---|
| Authors | Chan, PK2 Wai, A1 Philipsen, S1 TanUn, KC2 | ||||
| Issue Date | 2008 | ||||
| Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||
| Citation | Plos One, 2008, v. 3 n. 5 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0002134 | ||||
| Abstract | Hypersensitive site 5 (5′HS5) of the β-globin Locus Control Region functions as a developmental stage-specific border in erythroid cells. Here, we have analyzed the role of 59HS5 in the three dimensional organization of the β-gene locus using the Chromatin Conformation Capture (3C) technique. The results show that when 5′HS5 is deleted from the locus, both remote and internal regulatory elements are still able to interact with each other in a three-dimensional configuration termed the Active Chromatin Hub. Thus, the absence of 5′HS5 does not have an appreciable effect on the three dimensional organization of the β-globin locus. This rules out models in which 5′HS5 nucleates interactions with remote and/or internal regulatory elements. We also determined the binding of CTCF, the only defined insulator protein in mammalian cells, to 5′HS5 by using chromatin immunoprecipitation (ChIP) assays. We detect low levels of CTCF binding to 5′HS5 in primitive erythroid cells, in which it functions as a border element. Surprisingly, we also observe binding levels of CTCF to 5′HS5 in definitive erythroid cells. Thus, binding of CTCF to 5′HS5 per se does not render it a functional border element. This is consistent with the previous data suggesting that CTCF has dual functionality. © 2008 Chan et al. | ||||
| ISSN | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||
| DOI | http://dx.doi.org/10.1371/journal.pone.0002134 | ||||
| ISI Accession Number ID | WOS:000261642400055
Funding Information: This work was supported by the HKU School of Professional and Continuing Education (HKU SPACE). | ||||
| PubMed Central ID | PMC2358975 | ||||
| References | References in Scopus |
| dc.contributor.author | Chan, PK | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Wai, A | ||||
| dc.contributor.author | Philipsen, S | ||||
| dc.contributor.author | TanUn, KC | ||||
| dc.date.accessioned | 2010-09-06T09:54:18Z | ||||
| dc.date.available | 2010-09-06T09:54:18Z | ||||
| dc.date.issued | 2008 | ||||
| dc.description.abstract | Hypersensitive site 5 (5′HS5) of the β-globin Locus Control Region functions as a developmental stage-specific border in erythroid cells. Here, we have analyzed the role of 59HS5 in the three dimensional organization of the β-gene locus using the Chromatin Conformation Capture (3C) technique. The results show that when 5′HS5 is deleted from the locus, both remote and internal regulatory elements are still able to interact with each other in a three-dimensional configuration termed the Active Chromatin Hub. Thus, the absence of 5′HS5 does not have an appreciable effect on the three dimensional organization of the β-globin locus. This rules out models in which 5′HS5 nucleates interactions with remote and/or internal regulatory elements. We also determined the binding of CTCF, the only defined insulator protein in mammalian cells, to 5′HS5 by using chromatin immunoprecipitation (ChIP) assays. We detect low levels of CTCF binding to 5′HS5 in primitive erythroid cells, in which it functions as a border element. Surprisingly, we also observe binding levels of CTCF to 5′HS5 in definitive erythroid cells. Thus, binding of CTCF to 5′HS5 per se does not render it a functional border element. This is consistent with the previous data suggesting that CTCF has dual functionality. © 2008 Chan et al. | ||||
| dc.description.nature | published_or_final_version | ||||
| dc.identifier.citation | Plos One, 2008, v. 3 n. 5 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0002134 | ||||
| dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0002134 | ||||
| dc.identifier.epage | e2134 | ||||
| dc.identifier.hkuros | 142401 | ||||
| dc.identifier.isi | WOS:000261642400055
Funding Information: This work was supported by the HKU School of Professional and Continuing Education (HKU SPACE). | ||||
| dc.identifier.issn | 1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519 | ||||
| dc.identifier.issue | 5 | ||||
| dc.identifier.pmcid | PMC2358975 | ||||
| dc.identifier.pmid | 18461170 | ||||
| dc.identifier.scopus | eid_2-s2.0-47749114963 | ||||
| dc.identifier.spage | e2134 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/89238 | ||||
| dc.identifier.volume | 3 | ||||
| dc.language | eng | ||||
| dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | ||||
| dc.publisher.place | United States | ||||
| dc.relation.ispartof | PLoS ONE | ||||
| dc.relation.references | References in Scopus | ||||
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License | ||||
| dc.subject.mesh | Chromatin - genetics | ||||
| dc.subject.mesh | DNA-Binding Proteins - genetics | ||||
| dc.subject.mesh | Erythrocytes - physiology | ||||
| dc.subject.mesh | Globins - chemistry - genetics | ||||
| dc.subject.mesh | Locus Control Region - genetics | ||||
| dc.title | 5′ HS5 of the Human β-globin Locus Control Region is dispensable for the formation of the β-globin active chromatin hub | ||||
| dc.type | Article |
Author Affiliations
- Erasmus University Medical Center
- The University of Hong Kong