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Article: 5′ HS5 of the Human β-globin Locus Control Region is dispensable for the formation of the β-globin active chromatin hub

Title5′ HS5 of the Human β-globin Locus Control Region is dispensable for the formation of the β-globin active chromatin hub
Authors
Issue Date2008
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2008, v. 3 n. 5 How to Cite?
Abstract
Hypersensitive site 5 (5′HS5) of the β-globin Locus Control Region functions as a developmental stage-specific border in erythroid cells. Here, we have analyzed the role of 59HS5 in the three dimensional organization of the β-gene locus using the Chromatin Conformation Capture (3C) technique. The results show that when 5′HS5 is deleted from the locus, both remote and internal regulatory elements are still able to interact with each other in a three-dimensional configuration termed the Active Chromatin Hub. Thus, the absence of 5′HS5 does not have an appreciable effect on the three dimensional organization of the β-globin locus. This rules out models in which 5′HS5 nucleates interactions with remote and/or internal regulatory elements. We also determined the binding of CTCF, the only defined insulator protein in mammalian cells, to 5′HS5 by using chromatin immunoprecipitation (ChIP) assays. We detect low levels of CTCF binding to 5′HS5 in primitive erythroid cells, in which it functions as a border element. Surprisingly, we also observe binding levels of CTCF to 5′HS5 in definitive erythroid cells. Thus, binding of CTCF to 5′HS5 per se does not render it a functional border element. This is consistent with the previous data suggesting that CTCF has dual functionality. © 2008 Chan et al.
Persistent Identifierhttp://hdl.handle.net/10722/89238
ISSN
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
HKU School of Professional and Continuing Education (HKU SPACE)
Funding Information:

This work was supported by the HKU School of Professional and Continuing Education (HKU SPACE).

References

 

Author Affiliations
  1. Erasmus University Medical Center
  2. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorChan, PKen_HK
dc.contributor.authorWai, Aen_HK
dc.contributor.authorPhilipsen, Sen_HK
dc.contributor.authorTanUn, KCen_HK
dc.date.accessioned2010-09-06T09:54:18Z-
dc.date.available2010-09-06T09:54:18Z-
dc.date.issued2008en_HK
dc.identifier.citationPlos One, 2008, v. 3 n. 5en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89238-
dc.description.abstractHypersensitive site 5 (5′HS5) of the β-globin Locus Control Region functions as a developmental stage-specific border in erythroid cells. Here, we have analyzed the role of 59HS5 in the three dimensional organization of the β-gene locus using the Chromatin Conformation Capture (3C) technique. The results show that when 5′HS5 is deleted from the locus, both remote and internal regulatory elements are still able to interact with each other in a three-dimensional configuration termed the Active Chromatin Hub. Thus, the absence of 5′HS5 does not have an appreciable effect on the three dimensional organization of the β-globin locus. This rules out models in which 5′HS5 nucleates interactions with remote and/or internal regulatory elements. We also determined the binding of CTCF, the only defined insulator protein in mammalian cells, to 5′HS5 by using chromatin immunoprecipitation (ChIP) assays. We detect low levels of CTCF binding to 5′HS5 in primitive erythroid cells, in which it functions as a border element. Surprisingly, we also observe binding levels of CTCF to 5′HS5 in definitive erythroid cells. Thus, binding of CTCF to 5′HS5 per se does not render it a functional border element. This is consistent with the previous data suggesting that CTCF has dual functionality. © 2008 Chan et al.en_HK
dc.languageengen_HK
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshChromatin - genetics-
dc.subject.meshDNA-Binding Proteins - genetics-
dc.subject.meshErythrocytes - physiology-
dc.subject.meshGlobins - chemistry - genetics-
dc.subject.meshLocus Control Region - genetics-
dc.title5′ HS5 of the Human β-globin Locus Control Region is dispensable for the formation of the β-globin active chromatin huben_HK
dc.typeArticleen_HK
dc.identifier.emailTanUn, KC: kctanun@hkucc.hku.hken_HK
dc.identifier.authorityTanUn, KC=rp00787en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0002134en_HK
dc.identifier.pmid18461170en_HK
dc.identifier.pmcidPMC2358975-
dc.identifier.scopuseid_2-s2.0-47749114963en_HK
dc.identifier.hkuros142401en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-47749114963&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume3en_HK
dc.identifier.issue5en_HK
dc.identifier.spagee2134-
dc.identifier.epagee2134-
dc.identifier.eissn1932-6203-
dc.identifier.isiWOS:000261642400055-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, PK=24474084200en_HK
dc.identifier.scopusauthoridWai, A=6603455255en_HK
dc.identifier.scopusauthoridPhilipsen, S=7003745052en_HK
dc.identifier.scopusauthoridTanUn, KC=6602914262en_HK

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