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Article: Grass carp somatolactin: I. Evidence for PACAP induction of somatolactin-α and -β gene expression via activation of pituitary PAC-I receptors
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TitleGrass carp somatolactin: I. Evidence for PACAP induction of somatolactin-α and -β gene expression via activation of pituitary PAC-I receptors
 
AuthorsJiang, Q
Ko, WKW
Lerner, EA1
Chan, KM3
Wong, AOL2
 
KeywordsGrass carp pituitary cells
Pituitary adenylate cyclase-activating polypeptide
 
Issue Date2008
 
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpendo.physiology.org/
 
CitationAmerican Journal Of Physiology - Endocrinology And Metabolism, 2008, v. 295 n. 2, p. E463-E476 [How to Cite?]
DOI: http://dx.doi.org/10.1152/ajpendo.90385.2008
 
AbstractSomatolactin (SL), the latest member of the growth hormone/prolactin family, is a novel pituitary hormone with diverse functions. At present, SL can be identified only in fish but not in tetrapods and its regulation at the pituitary level has not been fully characterized. Using grass carp as a model, we examined the direct effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on SL secretion and synthesis at the pituitary cell level. As a first step, the structural identity of grass carp SL, SLα and SLβ, was established by 5′/3′-rapid amplification of cDNA ends. These two SL isoforms are single-copy genes and are expressed in two separate populations of pituitary cells located in the pars intermedia. In the carp pituitary, PACAP nerve fibers were detected in the nerve tracts of the neurohypophysis and extended into the vicinity of pituitary cells forming the pars intermedia. In primary cultures of grass carp pituitary cells, PACAP was effective in stimulating SL release, cellular SL content, and total SL production. The increase in SL production also occurred with parallel rises in SLα and SLβ mRNA levels. With the use of a combination of molecular and pharmacological approaches, PACAP-induced SL release and SL gene expression were shown to be mediated by pituitary PAC-I receptors. These findings, as a whole, suggest that PACAP may serve as a hypophysiotropic factor in fish stimulating SL secretion and synthesis at the pituitary level. Apparently, PACAP-induced SL production is mediated by upregulation of SLα and SLβ gene expression through activation of PAC-I receptors. Copyright © 2008 the American Physiological Society.
 
ISSN0193-1849
2013 Impact Factor: 4.088
 
DOIhttp://dx.doi.org/10.1152/ajpendo.90385.2008
 
ISI Accession Number IDWOS:000258133200030
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorJiang, Q
 
dc.contributor.authorKo, WKW
 
dc.contributor.authorLerner, EA
 
dc.contributor.authorChan, KM
 
dc.contributor.authorWong, AOL
 
dc.date.accessioned2010-09-06T09:54:13Z
 
dc.date.available2010-09-06T09:54:13Z
 
dc.date.issued2008
 
dc.description.abstractSomatolactin (SL), the latest member of the growth hormone/prolactin family, is a novel pituitary hormone with diverse functions. At present, SL can be identified only in fish but not in tetrapods and its regulation at the pituitary level has not been fully characterized. Using grass carp as a model, we examined the direct effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on SL secretion and synthesis at the pituitary cell level. As a first step, the structural identity of grass carp SL, SLα and SLβ, was established by 5′/3′-rapid amplification of cDNA ends. These two SL isoforms are single-copy genes and are expressed in two separate populations of pituitary cells located in the pars intermedia. In the carp pituitary, PACAP nerve fibers were detected in the nerve tracts of the neurohypophysis and extended into the vicinity of pituitary cells forming the pars intermedia. In primary cultures of grass carp pituitary cells, PACAP was effective in stimulating SL release, cellular SL content, and total SL production. The increase in SL production also occurred with parallel rises in SLα and SLβ mRNA levels. With the use of a combination of molecular and pharmacological approaches, PACAP-induced SL release and SL gene expression were shown to be mediated by pituitary PAC-I receptors. These findings, as a whole, suggest that PACAP may serve as a hypophysiotropic factor in fish stimulating SL secretion and synthesis at the pituitary level. Apparently, PACAP-induced SL production is mediated by upregulation of SLα and SLβ gene expression through activation of PAC-I receptors. Copyright © 2008 the American Physiological Society.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationAmerican Journal Of Physiology - Endocrinology And Metabolism, 2008, v. 295 n. 2, p. E463-E476 [How to Cite?]
DOI: http://dx.doi.org/10.1152/ajpendo.90385.2008
 
dc.identifier.doihttp://dx.doi.org/10.1152/ajpendo.90385.2008
 
dc.identifier.eissn1522-1555
 
dc.identifier.epageE476
 
dc.identifier.hkuros151020
 
dc.identifier.hkuros164869
 
dc.identifier.isiWOS:000258133200030
 
dc.identifier.issn0193-1849
2013 Impact Factor: 4.088
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.scopuseid_2-s2.0-52649161272
 
dc.identifier.spageE463
 
dc.identifier.urihttp://hdl.handle.net/10722/89231
 
dc.identifier.volume295
 
dc.languageeng
 
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpendo.physiology.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofAmerican Journal of Physiology - Endocrinology and Metabolism
 
dc.relation.referencesReferences in Scopus
 
dc.subjectGrass carp pituitary cells
 
dc.subjectPituitary adenylate cyclase-activating polypeptide
 
dc.titleGrass carp somatolactin: I. Evidence for PACAP induction of somatolactin-α and -β gene expression via activation of pituitary PAC-I receptors
 
dc.typeArticle
 
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Author Affiliations
  1. Massachusetts General Hospital
  2. The University of Hong Kong
  3. Chinese University of Hong Kong