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Article: Grass carp somatolactin: I. Evidence for PACAP induction of somatolactin-α and -β gene expression via activation of pituitary PAC-I receptors

TitleGrass carp somatolactin: I. Evidence for PACAP induction of somatolactin-α and -β gene expression via activation of pituitary PAC-I receptors
Authors
KeywordsGrass carp pituitary cells
Pituitary adenylate cyclase-activating polypeptide
Issue Date2008
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpendo.physiology.org/
Citation
American Journal Of Physiology - Endocrinology And Metabolism, 2008, v. 295 n. 2, p. E463-E476 How to Cite?
Abstract
Somatolactin (SL), the latest member of the growth hormone/prolactin family, is a novel pituitary hormone with diverse functions. At present, SL can be identified only in fish but not in tetrapods and its regulation at the pituitary level has not been fully characterized. Using grass carp as a model, we examined the direct effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on SL secretion and synthesis at the pituitary cell level. As a first step, the structural identity of grass carp SL, SLα and SLβ, was established by 5′/3′-rapid amplification of cDNA ends. These two SL isoforms are single-copy genes and are expressed in two separate populations of pituitary cells located in the pars intermedia. In the carp pituitary, PACAP nerve fibers were detected in the nerve tracts of the neurohypophysis and extended into the vicinity of pituitary cells forming the pars intermedia. In primary cultures of grass carp pituitary cells, PACAP was effective in stimulating SL release, cellular SL content, and total SL production. The increase in SL production also occurred with parallel rises in SLα and SLβ mRNA levels. With the use of a combination of molecular and pharmacological approaches, PACAP-induced SL release and SL gene expression were shown to be mediated by pituitary PAC-I receptors. These findings, as a whole, suggest that PACAP may serve as a hypophysiotropic factor in fish stimulating SL secretion and synthesis at the pituitary level. Apparently, PACAP-induced SL production is mediated by upregulation of SLα and SLβ gene expression through activation of PAC-I receptors. Copyright © 2008 the American Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/89231
ISSN
2013 Impact Factor: 4.088
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJiang, Qen_HK
dc.contributor.authorKo, WKWen_HK
dc.contributor.authorLerner, EAen_HK
dc.contributor.authorChan, KMen_HK
dc.contributor.authorWong, AOLen_HK
dc.date.accessioned2010-09-06T09:54:13Z-
dc.date.available2010-09-06T09:54:13Z-
dc.date.issued2008en_HK
dc.identifier.citationAmerican Journal Of Physiology - Endocrinology And Metabolism, 2008, v. 295 n. 2, p. E463-E476en_HK
dc.identifier.issn0193-1849en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89231-
dc.description.abstractSomatolactin (SL), the latest member of the growth hormone/prolactin family, is a novel pituitary hormone with diverse functions. At present, SL can be identified only in fish but not in tetrapods and its regulation at the pituitary level has not been fully characterized. Using grass carp as a model, we examined the direct effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on SL secretion and synthesis at the pituitary cell level. As a first step, the structural identity of grass carp SL, SLα and SLβ, was established by 5′/3′-rapid amplification of cDNA ends. These two SL isoforms are single-copy genes and are expressed in two separate populations of pituitary cells located in the pars intermedia. In the carp pituitary, PACAP nerve fibers were detected in the nerve tracts of the neurohypophysis and extended into the vicinity of pituitary cells forming the pars intermedia. In primary cultures of grass carp pituitary cells, PACAP was effective in stimulating SL release, cellular SL content, and total SL production. The increase in SL production also occurred with parallel rises in SLα and SLβ mRNA levels. With the use of a combination of molecular and pharmacological approaches, PACAP-induced SL release and SL gene expression were shown to be mediated by pituitary PAC-I receptors. These findings, as a whole, suggest that PACAP may serve as a hypophysiotropic factor in fish stimulating SL secretion and synthesis at the pituitary level. Apparently, PACAP-induced SL production is mediated by upregulation of SLα and SLβ gene expression through activation of PAC-I receptors. Copyright © 2008 the American Physiological Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpendo.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Endocrinology and Metabolismen_HK
dc.subjectGrass carp pituitary cellsen_HK
dc.subjectPituitary adenylate cyclase-activating polypeptideen_HK
dc.titleGrass carp somatolactin: I. Evidence for PACAP induction of somatolactin-α and -β gene expression via activation of pituitary PAC-I receptorsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0193-1849&volume=295&issue=2&spage=E463&epage=76&date=2008&atitle=Grass+carp+somatolactin:+I.+Evidence+for+PACAP+induction+of+somatolactin-{alpha]+and+-{beta}+gene+expression+via+activation+of+pituitary+PAC-I+receptorsen_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1152/ajpendo.90385.2008en_HK
dc.identifier.scopuseid_2-s2.0-52649161272en_HK
dc.identifier.hkuros151020en_HK
dc.identifier.hkuros164869-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-52649161272&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume295en_HK
dc.identifier.issue2en_HK
dc.identifier.spageE463en_HK
dc.identifier.epageE476en_HK
dc.identifier.eissn1522-1555-
dc.identifier.isiWOS:000258133200030-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridJiang, Q=35484035000en_HK
dc.identifier.scopusauthoridKo, WKW=7202286890en_HK
dc.identifier.scopusauthoridLerner, EA=7102531679en_HK
dc.identifier.scopusauthoridChan, KM=26642931000en_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK

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