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Article: Isolation and characterization of a novel haloacid permease from Burkholderia cepacia MBA4

TitleIsolation and characterization of a novel haloacid permease from Burkholderia cepacia MBA4
Authors
Issue Date2007
PublisherAmerican Society for Microbiology.
Citation
Applied And Environmental Microbiology, 2007, v. 73 n. 15, p. 4874-4880 How to Cite?
AbstractBurkholderia cepacia MBA4 is a bacterium that can utilize 2-haloacids as carbon and energy sources for growth. It has been proposed that dehalogenase-associated permease mediates the uptake of haloacid. In this paper, we report the first cloning and characterization of such a haloacid permease. The structural gene, designated deh4p, was found 353 bases downstream of the dehalogenase gene deh4a. Quantitative analysis of the expression of deh4p showed that it was induced by monochloroacetate (MCA), to a level similar to the MCA-induced level of deh4a. The nucleotide sequence of deh4p was determined, and an open reading frame of 1,656 bp encoding a putative peptide of 552 amino acids was identified. Deh4p has a putative molecular weight of 59,414 and an isoelectric point of 9.88. Deh4p has the signatures of sugar transport proteins and integral membrane proteins of the major facilitator superfamily. Uptake of [14C]MCA into the cell was Deh4p dependent. Deh4p has apparent K ms of 5.5 and 8.9 μM and Vmaxs of 9.1 and 23.1 nmol mg-1 min-1 for acetate and MCA, respectively. A mutant with a transposon-inactivated haloacid operon failed to grow on MCA even when deh4a was provided in trans. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/89228
ISSN
2015 Impact Factor: 3.823
2015 SCImago Journal Rankings: 1.891
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYu, Men_HK
dc.contributor.authorFaan, YWen_HK
dc.contributor.authorChung, WYKen_HK
dc.contributor.authorTsang, JSHen_HK
dc.date.accessioned2010-09-06T09:54:10Z-
dc.date.available2010-09-06T09:54:10Z-
dc.date.issued2007en_HK
dc.identifier.citationApplied And Environmental Microbiology, 2007, v. 73 n. 15, p. 4874-4880en_HK
dc.identifier.issn0099-2240en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89228-
dc.description.abstractBurkholderia cepacia MBA4 is a bacterium that can utilize 2-haloacids as carbon and energy sources for growth. It has been proposed that dehalogenase-associated permease mediates the uptake of haloacid. In this paper, we report the first cloning and characterization of such a haloacid permease. The structural gene, designated deh4p, was found 353 bases downstream of the dehalogenase gene deh4a. Quantitative analysis of the expression of deh4p showed that it was induced by monochloroacetate (MCA), to a level similar to the MCA-induced level of deh4a. The nucleotide sequence of deh4p was determined, and an open reading frame of 1,656 bp encoding a putative peptide of 552 amino acids was identified. Deh4p has a putative molecular weight of 59,414 and an isoelectric point of 9.88. Deh4p has the signatures of sugar transport proteins and integral membrane proteins of the major facilitator superfamily. Uptake of [14C]MCA into the cell was Deh4p dependent. Deh4p has apparent K ms of 5.5 and 8.9 μM and Vmaxs of 9.1 and 23.1 nmol mg-1 min-1 for acetate and MCA, respectively. A mutant with a transposon-inactivated haloacid operon failed to grow on MCA even when deh4a was provided in trans. Copyright © 2007, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofApplied and Environmental Microbiologyen_HK
dc.rightsApplied and Environmental Microbiology. Copyright © American Society for Microbiology.en_HK
dc.titleIsolation and characterization of a novel haloacid permease from Burkholderia cepacia MBA4en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0099-2240&volume=73&spage=4874&epage=4880&date=2007&atitle=Isolation+and+characterization+of+a+novel+haloacid+permease+from+Burkholderia+cepacia+MBA4.en_HK
dc.identifier.emailTsang, JSH: jshtsang@hku.hken_HK
dc.identifier.authorityTsang, JSH=rp00792en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/AEM.00576-07en_HK
dc.identifier.pmid17545323-
dc.identifier.scopuseid_2-s2.0-34547775053en_HK
dc.identifier.hkuros143122en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34547775053&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume73en_HK
dc.identifier.issue15en_HK
dc.identifier.spage4874en_HK
dc.identifier.epage4880en_HK
dc.identifier.eissn1098-5336-
dc.identifier.isiWOS:000248587600021-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYu, M=7404272859en_HK
dc.identifier.scopusauthoridFaan, YW=16244861000en_HK
dc.identifier.scopusauthoridChung, WYK=35080084100en_HK
dc.identifier.scopusauthoridTsang, JSH=7102483508en_HK

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