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Article: Complete sequence of plasmid pMP1 from the marine environmental Vibrio vulnificus and location of its replication origin

TitleComplete sequence of plasmid pMP1 from the marine environmental Vibrio vulnificus and location of its replication origin
Authors
Issue Date2009
PublisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/10126
Citation
Marine Biotechnology, 2009, v. 11, p. 465-462 How to Cite?
AbstractA novel cryptic plasmid, pMP1, from an environmental Vibrio vulnificus MP-4 isolated from Mai Po Nature Reserve in Hong Kong, has been characterized. The 7.6-kb plasmid had guanine–cytosine content of 40.03% and encoded four open reading frames (ORFs) with >100 amino acids. The predicted protein of ORF1 contained 478 amino acids showing 29% identity and 50% similarity over 309 amino acids to the integrase of Vibrio cholerae phage VP2. ORF2 encoded a putative protein of 596 amino acids, which were 23% identity and 42% similarity over 455 amino acids to the tail tape measure protein TP901 of Chromohalobacter salexigens phage. ORF3 and ORF4 encoded putative proteins of 103 and 287 amino acids, respectively, but showed no homologies to any known proteins. Further experiments indicated that a 3.2-kb fragment from EcoRI digestion could self-replicate. Analysis indicated that a sequence upstream of ORF4 had the features characteristic of theta-type replicons: AT-rich region, six potential direct repeats (iterons) spaced approximately two DNA helical turn apart (about 23 bp), two copies of 9 bp dnaA boxes, three Dam methylation sites, and five inverted repeats. Complementation experiments confirmed that the protein encoded by ORF4 was required for plasmid replication. We propose that ORF4 encode a new type of Rep protein and pMP1 is a new type of theta plasmid.
Persistent Identifierhttp://hdl.handle.net/10722/89221
ISSN
2015 Impact Factor: 3.062
2015 SCImago Journal Rankings: 1.184
ISI Accession Number ID
Funding AgencyGrant Number
The University of Hong Kong2002AA601160
RGC Central AllocationCA00/01.SC01
Funding Information:

This research was financially supported by 863 Project (no. 2002AA601160) supplement fund of The University of Hong Kong and RGC Central Allocation CA00/01.SC01. We would also like to thank the laboratory assistance of Miss Jessie Lai.

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ren_HK
dc.contributor.authorGu, JDen_HK
dc.date.accessioned2010-09-06T09:54:04Z-
dc.date.available2010-09-06T09:54:04Z-
dc.date.issued2009en_HK
dc.identifier.citationMarine Biotechnology, 2009, v. 11, p. 465-462en_HK
dc.identifier.issn1436-2228en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89221-
dc.description.abstractA novel cryptic plasmid, pMP1, from an environmental Vibrio vulnificus MP-4 isolated from Mai Po Nature Reserve in Hong Kong, has been characterized. The 7.6-kb plasmid had guanine–cytosine content of 40.03% and encoded four open reading frames (ORFs) with >100 amino acids. The predicted protein of ORF1 contained 478 amino acids showing 29% identity and 50% similarity over 309 amino acids to the integrase of Vibrio cholerae phage VP2. ORF2 encoded a putative protein of 596 amino acids, which were 23% identity and 42% similarity over 455 amino acids to the tail tape measure protein TP901 of Chromohalobacter salexigens phage. ORF3 and ORF4 encoded putative proteins of 103 and 287 amino acids, respectively, but showed no homologies to any known proteins. Further experiments indicated that a 3.2-kb fragment from EcoRI digestion could self-replicate. Analysis indicated that a sequence upstream of ORF4 had the features characteristic of theta-type replicons: AT-rich region, six potential direct repeats (iterons) spaced approximately two DNA helical turn apart (about 23 bp), two copies of 9 bp dnaA boxes, three Dam methylation sites, and five inverted repeats. Complementation experiments confirmed that the protein encoded by ORF4 was required for plasmid replication. We propose that ORF4 encode a new type of Rep protein and pMP1 is a new type of theta plasmid.-
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/10126en_HK
dc.relation.ispartofMarine Biotechnologyen_HK
dc.subject.meshBacterial Proteins - genetics-
dc.subject.meshGenes, Bacterial-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPlasmids - genetics-
dc.subject.meshReplication Origin-
dc.subject.meshVibrio vulnificus - genetics/isolation & purification-
dc.titleComplete sequence of plasmid pMP1 from the marine environmental Vibrio vulnificus and location of its replication originen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1436-2228&volume=11&spage=465&epage=462&date=2009&atitle=Complete+sequence+of+plasmid+pMP1+from+the+marine+environmental+Vibrio+vulnificus+and+location+of+its+replication+originen_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10126-008-9160-3-
dc.identifier.pmid19009320-
dc.identifier.scopuseid_2-s2.0-67349113968-
dc.identifier.hkuros160888en_HK
dc.identifier.isiWOS:000266333900004-
dc.identifier.citeulike3623634-

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