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Article: Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa

TitleProteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa
Authors
KeywordsProteomics
Issue Date2009
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.proteomesci.com/home/
Citation
Proteome Science, 2009, v. 7 article no. 44 How to Cite?
AbstractBACKGROUND: While the larval-juvenile transition (metamorphosis) in the spionid polychaete. Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle. RESULTS: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase). CONCLUSIONS: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.
Persistent Identifierhttp://hdl.handle.net/10722/89219
ISSN
2015 Impact Factor: 1.746
2015 SCImago Journal Rankings: 0.683
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
HKSAR Government662408
KAUST Global Partnership Program
Funding Information:

We are grateful to 3 anonymous reviewers for their constructive suggestions in improving the manuscript. We thank Dr. Priscilla Leung and Mr. Jack Man (Genome Research Centre, HKU) for MS analysis. We are also thankful to Dr. Kondethimma H. Chandramouli, Dr. Shawn M. Arellano, Dr. Olivia Ambrogio, and Dr. On On Lee for proof-reading the manuscript. This study was supported by the CAS/SAFEA International Partnership Program for Creative Research Teams, a research grant (GRF # 662408) from the HKSAR Government, and KAUST Global Partnership Program awarded to PY Qian.

 

DC FieldValueLanguage
dc.contributor.authorMok, Fen_HK
dc.contributor.authorThiyagarajan, Ven_HK
dc.contributor.authorQian, PYen_HK
dc.date.accessioned2010-09-06T09:54:03Z-
dc.date.available2010-09-06T09:54:03Z-
dc.date.issued2009en_HK
dc.identifier.citationProteome Science, 2009, v. 7 article no. 44en_HK
dc.identifier.issn1477-5956en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89219-
dc.description.abstractBACKGROUND: While the larval-juvenile transition (metamorphosis) in the spionid polychaete. Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle. RESULTS: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase). CONCLUSIONS: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms.-
dc.languageengen_HK
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.proteomesci.com/home/en_HK
dc.relation.ispartofProteome Scienceen_HK
dc.rightsProteome Science. Copyright © BioMed Central Ltd.en_HK
dc.subjectProteomics-
dc.titleProteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1477-5956&volume=7 article no. 44&spage=&epage=&date=2009&atitle=Proteomic+analysis+during+larval+development+and+metamorphosis+of+the+spionid+polychaete+Pseudopolydora+vexillosaen_HK
dc.identifier.emailMok, F: floramok@ust.hken_HK
dc.identifier.emailThiyagarajan, V: rajan@hkucc.hku.hk-
dc.identifier.authorityThiyagarajan, V=rp00796en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1477-5956-7-44-
dc.identifier.pmid20003395-
dc.identifier.pmcidPMC2803448-
dc.identifier.scopuseid_2-s2.0-74049109438-
dc.identifier.hkuros163879en_HK
dc.identifier.hkuros177900-
dc.identifier.volume7 article no. 44-
dc.identifier.isiWOS:000273444500001-
dc.publisher.placeUnited Kingdom-
dc.identifier.scopusauthoridMok, FSY=23566932300-
dc.identifier.scopusauthoridThiyagarajan, V=6602476830-
dc.identifier.scopusauthoridQian, PY=35240648600-
dc.identifier.citeulike6374944-

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