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Article: Grass carp somatolactin: II. Pharmacological study on postreceptor signaling mechanisms for PACAP-induced somatolactin-α and -β gene expression

TitleGrass carp somatolactin: II. Pharmacological study on postreceptor signaling mechanisms for PACAP-induced somatolactin-α and -β gene expression
Authors
KeywordsGrass carp pituitary cells
Pituitary adenylate cyclase-activating polypeptide
Issue Date2008
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpendo.physiology.org/
Citation
American Journal Of Physiology - Endocrinology And Metabolism, 2008, v. 295 n. 2, p. E477-E490 How to Cite?
Abstract
Somatolactin (SL), the latest member of the growth hormone/prolactin family, is a novel pituitary hormone with diverse functions. However, the signal transduction mechanisms responsible for SL expression are still largely unknown. Using grass carp as an animal model, we examined the direct effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on SL gene expression at the pituitary level. In primary cultures of grass carp pituitary cells, SLα and SLδ mRNA levels could be elevated by PACAP via activation of PAC-I receptors. With the use of a pharmacological approach, the AC/cAMP/PKA and PLC/inositol 1,4,5-trisphosphate (IP3)/PKC pathways and subsequent activation of the Ca 2+/calmodulin (CaM)/CaMK-II cascades were shown to be involved in PACAP-induced SLα mRNA expression. Apparently, the downstream Ca 2+/CaM-dependent cascades were triggered by extracellular Ca 2+ ([Ca 2+] e) entry via L-type voltage-sensitive Ca 2+ channels (VSCC) and Ca 2+ release from IP3-sensitive intracellular Ca 2+ stores. In addition, the VSCC component could be activated by cAMP/PKA- and PLC/PKC-dependent mechanisms. Similar postreceptor signaling cascades were also observed for PACAP-induced SLβ mRNA expression, except that [Ca 2+] e entry through VSCC, PKC coupling to PLC, and subsequent activation of CaMK-II were not involved. These findings, taken together, provide evidence for the first time that PACAP can induce SLα and SLδ gene expression in fish model via PAC-I receptors through differential coupling to overlapping and yet distinct signaling pathways. Copyright © 2008 the American Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/89191
ISSN
2013 Impact Factor: 4.088
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorJiang, Qen_HK
dc.contributor.authorHe, Men_HK
dc.contributor.authorWang, Xen_HK
dc.contributor.authorWong, AOLen_HK
dc.date.accessioned2010-09-06T09:53:40Z-
dc.date.available2010-09-06T09:53:40Z-
dc.date.issued2008en_HK
dc.identifier.citationAmerican Journal Of Physiology - Endocrinology And Metabolism, 2008, v. 295 n. 2, p. E477-E490en_HK
dc.identifier.issn0193-1849en_HK
dc.identifier.urihttp://hdl.handle.net/10722/89191-
dc.description.abstractSomatolactin (SL), the latest member of the growth hormone/prolactin family, is a novel pituitary hormone with diverse functions. However, the signal transduction mechanisms responsible for SL expression are still largely unknown. Using grass carp as an animal model, we examined the direct effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on SL gene expression at the pituitary level. In primary cultures of grass carp pituitary cells, SLα and SLδ mRNA levels could be elevated by PACAP via activation of PAC-I receptors. With the use of a pharmacological approach, the AC/cAMP/PKA and PLC/inositol 1,4,5-trisphosphate (IP3)/PKC pathways and subsequent activation of the Ca 2+/calmodulin (CaM)/CaMK-II cascades were shown to be involved in PACAP-induced SLα mRNA expression. Apparently, the downstream Ca 2+/CaM-dependent cascades were triggered by extracellular Ca 2+ ([Ca 2+] e) entry via L-type voltage-sensitive Ca 2+ channels (VSCC) and Ca 2+ release from IP3-sensitive intracellular Ca 2+ stores. In addition, the VSCC component could be activated by cAMP/PKA- and PLC/PKC-dependent mechanisms. Similar postreceptor signaling cascades were also observed for PACAP-induced SLβ mRNA expression, except that [Ca 2+] e entry through VSCC, PKC coupling to PLC, and subsequent activation of CaMK-II were not involved. These findings, taken together, provide evidence for the first time that PACAP can induce SLα and SLδ gene expression in fish model via PAC-I receptors through differential coupling to overlapping and yet distinct signaling pathways. Copyright © 2008 the American Physiological Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpendo.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Endocrinology and Metabolismen_HK
dc.subjectGrass carp pituitary cellsen_HK
dc.subjectPituitary adenylate cyclase-activating polypeptideen_HK
dc.titleGrass carp somatolactin: II. Pharmacological study on postreceptor signaling mechanisms for PACAP-induced somatolactin-α and -β gene expressionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0193-1849&volume=295&issue=2&spage=E477&epage=90&date=2008&atitle=Grass+carp+somatolactin:+II.+Pharmacological+study+on+postreceptor+signaling+mechanisms+for+PACAP-induced+somatolactin-{alpha}+and+-{beta}+gene+expressionen_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1152/ajpendo.90386.2008en_HK
dc.identifier.scopuseid_2-s2.0-52649086807en_HK
dc.identifier.hkuros151032en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-52649086807&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume295en_HK
dc.identifier.issue2en_HK
dc.identifier.spageE477en_HK
dc.identifier.epageE490en_HK
dc.identifier.eissn1522-1555-
dc.identifier.isiWOS:000258133200031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridJiang, Q=35484035000en_HK
dc.identifier.scopusauthoridHe, M=7402609178en_HK
dc.identifier.scopusauthoridWang, X=52964763100en_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK

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