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Article: High resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell lines

TitleHigh resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell lines
Authors
KeywordsAdenocarcinoma
Array CGH
EEF1A2
KLF6
Lung
Metaphase CGH
Tobacco
Issue Date2007
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
Citation
Cancer Letters, 2007, v. 245 n. 1-2, p. 303-314 How to Cite?
AbstractTumours develop from clonally expanded population of cells harbouring aberrations of oncogenes and tumour suppressor genes. In this study, metaphase and array comparative genomic hybridization showed good correlation of aberration profiles in lung adenocarcinoma cell lines from patients with different tobacco exposure. Recurrent DNA gains were found at chromosomes 1, 7, 8, 17, 20, and deletions at 1, 3, 8, 9, 10, 12, 17, 18, 19. Candidate tumour loci and encompassed genes at 7p21 (AGR2), 8q21(TPD52), 20q13 (ZNF217, WFDC2, EEF1A2) and 10p15 (KLF6) were analyzed by dual colour FISH for genomic changes and quantitative PCR for expression changes. Results indicated that EEF1A2 and KLF6 were strong candidates of oncogene and tumour suppressor genes, respectively. This study illustrates, a practical strategy for identifying candidate cancer genes from microarray data. © 2006 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/88789
ISSN
2015 Impact Factor: 5.992
2015 SCImago Journal Rankings: 2.331
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhu, Hen_HK
dc.contributor.authorLam, DCLen_HK
dc.contributor.authorHan, KCen_HK
dc.contributor.authorTin, VPCen_HK
dc.contributor.authorSuen, WSen_HK
dc.contributor.authorWang, Een_HK
dc.contributor.authorLam, WKen_HK
dc.contributor.authorCai, WWen_HK
dc.contributor.authorChung, LPen_HK
dc.contributor.authorWong, MPen_HK
dc.date.accessioned2010-09-06T09:48:01Z-
dc.date.available2010-09-06T09:48:01Z-
dc.date.issued2007en_HK
dc.identifier.citationCancer Letters, 2007, v. 245 n. 1-2, p. 303-314en_HK
dc.identifier.issn0304-3835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88789-
dc.description.abstractTumours develop from clonally expanded population of cells harbouring aberrations of oncogenes and tumour suppressor genes. In this study, metaphase and array comparative genomic hybridization showed good correlation of aberration profiles in lung adenocarcinoma cell lines from patients with different tobacco exposure. Recurrent DNA gains were found at chromosomes 1, 7, 8, 17, 20, and deletions at 1, 3, 8, 9, 10, 12, 17, 18, 19. Candidate tumour loci and encompassed genes at 7p21 (AGR2), 8q21(TPD52), 20q13 (ZNF217, WFDC2, EEF1A2) and 10p15 (KLF6) were analyzed by dual colour FISH for genomic changes and quantitative PCR for expression changes. Results indicated that EEF1A2 and KLF6 were strong candidates of oncogene and tumour suppressor genes, respectively. This study illustrates, a practical strategy for identifying candidate cancer genes from microarray data. © 2006 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canleten_HK
dc.relation.ispartofCancer Lettersen_HK
dc.rightsCancer Letters. Copyright © Elsevier Ireland Ltd.en_HK
dc.subjectAdenocarcinomaen_HK
dc.subjectArray CGHen_HK
dc.subjectEEF1A2en_HK
dc.subjectKLF6en_HK
dc.subjectLungen_HK
dc.subjectMetaphase CGHen_HK
dc.subjectTobaccoen_HK
dc.subject.meshAdenocarcinoma - genetics - pathologyen_HK
dc.subject.meshAgeden_HK
dc.subject.meshChromosome Aberrationsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Dosageen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshGenetic Predisposition to Disease - geneticsen_HK
dc.subject.meshGenome, Humanen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIn Situ Hybridization, Fluorescenceen_HK
dc.subject.meshLung Neoplasms - genetics - pathologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMetaphase - geneticsen_HK
dc.subject.meshMicroarray Analysis - methodsen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshNucleic Acid Hybridization - methodsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleHigh resolution analysis of genomic aberrations by metaphase and array comparative genomic hybridization identifies candidate tumour genes in lung cancer cell linesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0304-3835&volume=245&issue=1-2&spage=303&epage=14&date=2006&atitle=High+resolution+analysis+of+genomic+aberrations+by+metaphase+and+array+comparative+genomic+hybridization+identifies+candidate+tumour+genes+in+lung+cancer+cell+linesen_HK
dc.identifier.emailLam, DCL: dcllam@hku.hken_HK
dc.identifier.emailChung, LP: lpchung@hkucc.hku.hken_HK
dc.identifier.emailWong, MP: mwpik@hkucc.hku.hken_HK
dc.identifier.authorityLam, DCL=rp01345en_HK
dc.identifier.authorityChung, LP=rp00249en_HK
dc.identifier.authorityWong, MP=rp00348en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.canlet.2006.01.020en_HK
dc.identifier.pmid16517066-
dc.identifier.scopuseid_2-s2.0-33845651679en_HK
dc.identifier.hkuros121244en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33845651679&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume245en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage303en_HK
dc.identifier.epage314en_HK
dc.identifier.isiWOS:000244146100035-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridZhu, H=37109922200en_HK
dc.identifier.scopusauthoridLam, DCL=7201749615en_HK
dc.identifier.scopusauthoridHan, KC=40161408700en_HK
dc.identifier.scopusauthoridTin, VPC=6603199735en_HK
dc.identifier.scopusauthoridSuen, WS=12786607600en_HK
dc.identifier.scopusauthoridWang, E=7403414620en_HK
dc.identifier.scopusauthoridLam, WK=7203021937en_HK
dc.identifier.scopusauthoridCai, WW=7401710584en_HK
dc.identifier.scopusauthoridChung, LP=24315879100en_HK
dc.identifier.scopusauthoridWong, MP=7403907887en_HK
dc.identifier.citeulike539241-

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