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Article: Identification of cytotoxic T cell epitopes within Epstein-Barr virus (EBV) oncogene latent membrane protein 1 (LMP1): Evidence for HLA A2 supertype-restricted immune recognition of EBV-infected cells by LMP1-specific cytotoxic T lymphocytes

TitleIdentification of cytotoxic T cell epitopes within Epstein-Barr virus (EBV) oncogene latent membrane protein 1 (LMP1): Evidence for HLA A2 supertype-restricted immune recognition of EBV-infected cells by LMP1-specific cytotoxic T lymphocytes
Authors
Issue Date1998
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.de
Citation
European Journal Of Immunology, 1998, v. 28 n. 2, p. 451-458 How to Cite?
AbstractEpstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) and latent membrane proteins (LMP) are the only antigens consistently expressed in malignancies such as nasopharyngeal carcinoma (NPC) and Hodgkin's disease (HD). Since EBNA1 is not recognized by EBV-specific cytotoxic T lymphocytes (CTL), there is increasing interest in the identification of the potential target epitopes within LMP1. Although LMP1-specific CTL have been isolated from seropositive individuals, earlier attempts to identify the peptide epitopes recognized by these T cells have been unsuccessful. In the present report we used a novel protocol to identify CTL epitopes within LMP1 which can be recognized by both polyclonal and clonal CTL. Firstly, a computer-based program was employed to identify the potential HLA-binding peptides within LMP1. Polyclonal CD8+ CTL were then isolated from seropositive donors that recognized the peptide epitopes YLLEMLWRL and YLQQNWWTL from LMP1 in association with HLA A2. Limiting dilution analysis of the memory CTL response revealed that the LMP1-specific CTL response constitutes a minor component of the CTL response in healthy virus carriers. Interestingly, analysis of YLLEMLWRL-specific CTL revealed that these CTL were able to lyse EBV-infected B cells expressing different HLA A2 supertype alleles including A(*)0201, A(*)0202, A(*)0203, A(*)0204, A(*)0206, A(*)6802 and A(*)6901. These data strongly support the notion that HLA class I supertype-restricted CTL may be of significant use in the development of peptide-based immunotherapeutics against EBV-associated malignancies in different ethnic populations.
Persistent Identifierhttp://hdl.handle.net/10722/88728
ISSN
2015 Impact Factor: 4.179
2015 SCImago Journal Rankings: 2.568
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKhanna, Ren_HK
dc.contributor.authorBurrows, SRen_HK
dc.contributor.authorNicholls, Jen_HK
dc.contributor.authorPoulsen, LMen_HK
dc.date.accessioned2010-09-06T09:47:11Z-
dc.date.available2010-09-06T09:47:11Z-
dc.date.issued1998en_HK
dc.identifier.citationEuropean Journal Of Immunology, 1998, v. 28 n. 2, p. 451-458en_HK
dc.identifier.issn0014-2980en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88728-
dc.description.abstractEpstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) and latent membrane proteins (LMP) are the only antigens consistently expressed in malignancies such as nasopharyngeal carcinoma (NPC) and Hodgkin's disease (HD). Since EBNA1 is not recognized by EBV-specific cytotoxic T lymphocytes (CTL), there is increasing interest in the identification of the potential target epitopes within LMP1. Although LMP1-specific CTL have been isolated from seropositive individuals, earlier attempts to identify the peptide epitopes recognized by these T cells have been unsuccessful. In the present report we used a novel protocol to identify CTL epitopes within LMP1 which can be recognized by both polyclonal and clonal CTL. Firstly, a computer-based program was employed to identify the potential HLA-binding peptides within LMP1. Polyclonal CD8+ CTL were then isolated from seropositive donors that recognized the peptide epitopes YLLEMLWRL and YLQQNWWTL from LMP1 in association with HLA A2. Limiting dilution analysis of the memory CTL response revealed that the LMP1-specific CTL response constitutes a minor component of the CTL response in healthy virus carriers. Interestingly, analysis of YLLEMLWRL-specific CTL revealed that these CTL were able to lyse EBV-infected B cells expressing different HLA A2 supertype alleles including A(*)0201, A(*)0202, A(*)0203, A(*)0204, A(*)0206, A(*)6802 and A(*)6901. These data strongly support the notion that HLA class I supertype-restricted CTL may be of significant use in the development of peptide-based immunotherapeutics against EBV-associated malignancies in different ethnic populations.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.deen_HK
dc.relation.ispartofEuropean Journal of Immunologyen_HK
dc.subject.meshAllelesen_HK
dc.subject.meshBurkitt Lymphoma - immunologyen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshEpitopes, T-Lymphocyte - analysis - chemistryen_HK
dc.subject.meshHLA-A2 Antigen - genetics - immunology - metabolismen_HK
dc.subject.meshHerpesvirus 4, Human - immunologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLymphocyte Activationen_HK
dc.subject.meshOncogene Proteins, Viral - immunologyen_HK
dc.subject.meshPeptide Fragments - immunology - metabolismen_HK
dc.subject.meshProtein Binding - immunologyen_HK
dc.subject.meshT-Lymphocytes, Cytotoxic - immunology - metabolismen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.subject.meshTumor Virus Infections - immunologyen_HK
dc.subject.meshViral Matrix Proteins - immunology - metabolismen_HK
dc.titleIdentification of cytotoxic T cell epitopes within Epstein-Barr virus (EBV) oncogene latent membrane protein 1 (LMP1): Evidence for HLA A2 supertype-restricted immune recognition of EBV-infected cells by LMP1-specific cytotoxic T lymphocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-2980&volume=28&spage=451&epage=458&date=1998&atitle=Identification+of+cytotoxic+T+cell+epitopes+within+Epstein-Barr+virus+(EBV)+oncogene+latent+membrane+protein+1+(LMP1):+evidence+for+HLA+A2+supertype-restricted+immune+recognition+of+EBV-infected+cells+by+LMP1-specific+cytotoxic+T+lymphocytesen_HK
dc.identifier.emailNicholls, J:nicholls@pathology.hku.hken_HK
dc.identifier.authorityNicholls, J=rp00364en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1521-4141(199802)28:02<451::AID-IMMU451>3.0.CO;2-Uen_HK
dc.identifier.pmid9521052-
dc.identifier.scopuseid_2-s2.0-0031936036en_HK
dc.identifier.hkuros34030en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031936036&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue2en_HK
dc.identifier.spage451en_HK
dc.identifier.epage458en_HK
dc.identifier.isiWOS:000072307700006-
dc.publisher.placeGermanyen_HK

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