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Article: Cloning and characterization of the promoter region of the mouse frizzled-related protein 4 gene

TitleCloning and characterization of the promoter region of the mouse frizzled-related protein 4 gene
Authors
Issue Date2003
PublisherWalter de Gruyter GmbH & Co KG. The Journal's web site is located at http://www.degruyter.de/journals/bc
Citation
Biological Chemistry, 2003, v. 384 n. 8, p. 1147-1154 How to Cite?
AbstractFrizzled-related protein (Frp) is a newly identified family of secreted proteins involved in the Wnt signaling pathway. To date, little is known about the underlying mechanisms regulating Frp expression. In this study the promoter region of mouse frizzled related protein 4 (sFrp4) gene was cloned, sequenced, and analyzed using transient reporter assays along with site-directed mutagenesis. Two clusters of cis-acting elements, STAT3/Lyf-1/MZF1 (site 1) and C/EBP-β/ GATA-1/CREB (site 2) located in the promoter region from - 238 to - 144 were found to be essential for the promoter activity of sFrp4. In addition to sites 1 and 2, putative transcriptional factor binding sites for TFIID, SP1/GC and ATF/CREB exhibited positive, while the site for NRSE exhibited negative regulatory functions, as determined by the alkaline phosphatase activities of the reporter assay. We also demonstrate that the ATF/CREB site may cooperatively interact with the NRSF-like element in regulating sFrp4 promoter activity. The data of our study, which is the first promoter analysis of mouse Frp genes, provide the basis for understanding the functions and the regulation of Frp and its role in regulating Wnt signals.
Persistent Identifierhttp://hdl.handle.net/10722/88647
ISSN
2015 Impact Factor: 2.71
2015 SCImago Journal Rankings: 1.607
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWai Wong, VKen_HK
dc.contributor.authorPing Yam, JWen_HK
dc.contributor.authorHsiao, WLWen_HK
dc.date.accessioned2010-09-06T09:46:07Z-
dc.date.available2010-09-06T09:46:07Z-
dc.date.issued2003en_HK
dc.identifier.citationBiological Chemistry, 2003, v. 384 n. 8, p. 1147-1154en_HK
dc.identifier.issn1431-6730en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88647-
dc.description.abstractFrizzled-related protein (Frp) is a newly identified family of secreted proteins involved in the Wnt signaling pathway. To date, little is known about the underlying mechanisms regulating Frp expression. In this study the promoter region of mouse frizzled related protein 4 (sFrp4) gene was cloned, sequenced, and analyzed using transient reporter assays along with site-directed mutagenesis. Two clusters of cis-acting elements, STAT3/Lyf-1/MZF1 (site 1) and C/EBP-β/ GATA-1/CREB (site 2) located in the promoter region from - 238 to - 144 were found to be essential for the promoter activity of sFrp4. In addition to sites 1 and 2, putative transcriptional factor binding sites for TFIID, SP1/GC and ATF/CREB exhibited positive, while the site for NRSE exhibited negative regulatory functions, as determined by the alkaline phosphatase activities of the reporter assay. We also demonstrate that the ATF/CREB site may cooperatively interact with the NRSF-like element in regulating sFrp4 promoter activity. The data of our study, which is the first promoter analysis of mouse Frp genes, provide the basis for understanding the functions and the regulation of Frp and its role in regulating Wnt signals.en_HK
dc.languageengen_HK
dc.publisherWalter de Gruyter GmbH & Co KG. The Journal's web site is located at http://www.degruyter.de/journals/bcen_HK
dc.relation.ispartofBiological Chemistryen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshDNAen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshMutationen_HK
dc.subject.meshPromoter Regions, Geneticen_HK
dc.subject.meshProteins - geneticsen_HK
dc.subject.meshRatsen_HK
dc.subject.meshSequence Alignmenten_HK
dc.subject.meshSignal Transductionen_HK
dc.titleCloning and characterization of the promoter region of the mouse frizzled-related protein 4 geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1431-6730&volume=384&issue=8&spage=1147&epage=1154 &date=2003&atitle=Cloning+and+characterization+of+the+promoter+region+of+the+mouse+frizzled-related+protein+4+geneen_HK
dc.identifier.emailPing Yam, JW:judyyam@pathology.hku.hken_HK
dc.identifier.authorityPing Yam, JW=rp00468en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1515/BC.2003.127en_HK
dc.identifier.pmid12974383-
dc.identifier.scopuseid_2-s2.0-0141626773en_HK
dc.identifier.hkuros86121en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0141626773&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume384en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1147en_HK
dc.identifier.epage1154en_HK
dc.identifier.isiWOS:000184962700003-
dc.publisher.placeGermanyen_HK

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