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Article: Epstein-Barr virus expression within keratinizing nasopharyngeal carcinoma

TitleEpstein-Barr virus expression within keratinizing nasopharyngeal carcinoma
Authors
KeywordsEBV expression
NPC differentiation
Issue Date1998
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
Citation
Journal Of Medical Virology, 1998, v. 55 n. 3, p. 227-233 How to Cite?
AbstractThree stages of maturation can be seen in keratinizing nasopharyngeal carcinomas. These stages are similar morphologically to basal cells, intermediate and superficial squamous cells seen in normal squamous epithelium. Taking advantage of such a diverse tumour cell population, 10 keratinizing nasopharyngeal carcinoma (NPC) were examined by in situ hybridization for the presence of latent Epstein-Barr Virus (EBV) using EBV encoded RNAs (EBERs) and by immunohistology for the presence of EBV early antigen-diffuse (EA-D) and the 350/220 kd membrane glycoprotein of the EBV. The basal cell-like tumour cells are mainly infected latently with the virus; viral replication was found in isolated intermediate squamous cells, whilst superficial squamous cells are largely depleted of all the viral markers. We used a control series of nonkeratinizing nasopharyngeal carcinomas composed of undifferentiated and poorly differentiated tumour cells and EBV latency was present in these tumours. Viral replication was detected by RT-PCR, in the undifferentiated tumours but viral replication was not seen by immunohistology. The possible relationship between EBV life cycle in these tumours and tumour cell differentiation is discussed in the light of these findings.
Persistent Identifierhttp://hdl.handle.net/10722/88571
ISSN
2015 Impact Factor: 1.998
2015 SCImago Journal Rankings: 1.015
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, JXen_HK
dc.contributor.authorChen, HLen_HK
dc.contributor.authorZong, YSen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorNicholls, Jen_HK
dc.contributor.authorMiddeldorp, JMen_HK
dc.contributor.authorSham, JSTen_HK
dc.contributor.authorGriffin, BEen_HK
dc.contributor.authorNg, MHen_HK
dc.date.accessioned2010-09-06T09:45:06Z-
dc.date.available2010-09-06T09:45:06Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Medical Virology, 1998, v. 55 n. 3, p. 227-233en_HK
dc.identifier.issn0146-6615en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88571-
dc.description.abstractThree stages of maturation can be seen in keratinizing nasopharyngeal carcinomas. These stages are similar morphologically to basal cells, intermediate and superficial squamous cells seen in normal squamous epithelium. Taking advantage of such a diverse tumour cell population, 10 keratinizing nasopharyngeal carcinoma (NPC) were examined by in situ hybridization for the presence of latent Epstein-Barr Virus (EBV) using EBV encoded RNAs (EBERs) and by immunohistology for the presence of EBV early antigen-diffuse (EA-D) and the 350/220 kd membrane glycoprotein of the EBV. The basal cell-like tumour cells are mainly infected latently with the virus; viral replication was found in isolated intermediate squamous cells, whilst superficial squamous cells are largely depleted of all the viral markers. We used a control series of nonkeratinizing nasopharyngeal carcinomas composed of undifferentiated and poorly differentiated tumour cells and EBV latency was present in these tumours. Viral replication was detected by RT-PCR, in the undifferentiated tumours but viral replication was not seen by immunohistology. The possible relationship between EBV life cycle in these tumours and tumour cell differentiation is discussed in the light of these findings.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763en_HK
dc.relation.ispartofJournal of Medical Virologyen_HK
dc.rightsJournal of Medical Virology. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectEBV expressionen_HK
dc.subjectNPC differentiationen_HK
dc.subject.meshAntibodies, Monoclonalen_HK
dc.subject.meshAntigens, Viral - analysisen_HK
dc.subject.meshCarcinoma, Squamous Cell - pathology - virologyen_HK
dc.subject.meshCell Differentiationen_HK
dc.subject.meshGenes, Viralen_HK
dc.subject.meshHerpesvirus 4, Human - genetics - immunology - physiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshNasopharyngeal Neoplasms - pathology - virologyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshViral Matrix Proteins - analysisen_HK
dc.subject.meshVirus Latencyen_HK
dc.subject.meshVirus Replicationen_HK
dc.titleEpstein-Barr virus expression within keratinizing nasopharyngeal carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0146-6615&volume=55&spage=227&epage=233&date=1998&atitle=Epstein-Barr+virus+expression+within+keratinizing+nasopharyngeal+carcinomaen_HK
dc.identifier.emailZhang, JX:zhangajx@hkucc.hku.hken_HK
dc.identifier.emailChen, HL:hlchen@hkucc.hku.hken_HK
dc.identifier.emailNicholls, J:nicholls@pathology.hku.hken_HK
dc.identifier.authorityZhang, JX=rp00413en_HK
dc.identifier.authorityChen, HL=rp00383en_HK
dc.identifier.authorityNicholls, J=rp00364en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1096-9071(199807)55:3<227::AID-JMV8>3.0.CO;2-3en_HK
dc.identifier.pmid9624611-
dc.identifier.scopuseid_2-s2.0-0031812716en_HK
dc.identifier.hkuros34023en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031812716&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume55en_HK
dc.identifier.issue3en_HK
dc.identifier.spage227en_HK
dc.identifier.epage233en_HK
dc.identifier.isiWOS:000074002700008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, JX=12752135600en_HK
dc.identifier.scopusauthoridChen, HL=26643315400en_HK
dc.identifier.scopusauthoridZong, YS=7005203474en_HK
dc.identifier.scopusauthoridChan, KH=35338760600en_HK
dc.identifier.scopusauthoridNicholls, J=7201463077en_HK
dc.identifier.scopusauthoridMiddeldorp, JM=35493462100en_HK
dc.identifier.scopusauthoridSham, JST=24472255400en_HK
dc.identifier.scopusauthoridGriffin, BE=7201402684en_HK
dc.identifier.scopusauthoridNg, MH=7202076421en_HK

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