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Article: Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis

TitleUp-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis
Authors
KeywordsIL-6
IL-8
MIF
NEC
Issue Date2005
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HIS
Citation
Histopathology, 2005, v. 46 n. 6, p. 659-667 How to Cite?
AbstractAims: To investigate the role of macrophage migration inhibitory factor (MIF) and its downstream cytokine cascade in necrotizing enterocolitis (NEC). Methods and results: The expression of MIF mRNA and protein in NEC guts was assayed by in situ hybridization and immunohistochemistry, respectively. Concentrations of MIF, interleukin (IL)-6 and IL-8 in the serum and in the: supernatant of macrophage cultures were examined by ELISA. Increased expression of MIF mRNA and protein was observed in the NEC guts, mainly in the infiltrating macrophages in the mucosa and submucosal layers. Up-regulation of MIF was associated with the accumulation of macrophages and T cells. In addition, serum levels of MIF, IL-6 and IL-8 in NEC patients during the acute stage of the disease were significantly increased. The expression of MIF decreased both locally and systemically after the disease was resolved. MIF was also found to increase the secretion of IL-6 and IL-8 by macrophages isolated from healthy individuals in vitro in NEC. Conclusions: MIF acts by stimulating macrophage production of IL-6 and IL-8. This further aggravates the inflammatory process by increasing the infiltration of neutrophils and activating inflammatory cells. The results of this study suggest that MIF plays an important role in the pathogenesis of NEC and may serve as a target for therapeutic intervention in NEC. © 2005 Blackwell Publishing Limited.
Persistent Identifierhttp://hdl.handle.net/10722/88548
ISSN
2015 Impact Factor: 3.425
2015 SCImago Journal Rankings: 1.488
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRen, Yen_HK
dc.contributor.authorLin, CLen_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorChen, XYen_HK
dc.contributor.authorHuang, Xen_HK
dc.contributor.authorLui, Ven_HK
dc.contributor.authorNicholls, Jen_HK
dc.contributor.authorLan, HYen_HK
dc.contributor.authorTam, PKHen_HK
dc.date.accessioned2010-09-06T09:44:48Z-
dc.date.available2010-09-06T09:44:48Z-
dc.date.issued2005en_HK
dc.identifier.citationHistopathology, 2005, v. 46 n. 6, p. 659-667en_HK
dc.identifier.issn0309-0167en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88548-
dc.description.abstractAims: To investigate the role of macrophage migration inhibitory factor (MIF) and its downstream cytokine cascade in necrotizing enterocolitis (NEC). Methods and results: The expression of MIF mRNA and protein in NEC guts was assayed by in situ hybridization and immunohistochemistry, respectively. Concentrations of MIF, interleukin (IL)-6 and IL-8 in the serum and in the: supernatant of macrophage cultures were examined by ELISA. Increased expression of MIF mRNA and protein was observed in the NEC guts, mainly in the infiltrating macrophages in the mucosa and submucosal layers. Up-regulation of MIF was associated with the accumulation of macrophages and T cells. In addition, serum levels of MIF, IL-6 and IL-8 in NEC patients during the acute stage of the disease were significantly increased. The expression of MIF decreased both locally and systemically after the disease was resolved. MIF was also found to increase the secretion of IL-6 and IL-8 by macrophages isolated from healthy individuals in vitro in NEC. Conclusions: MIF acts by stimulating macrophage production of IL-6 and IL-8. This further aggravates the inflammatory process by increasing the infiltration of neutrophils and activating inflammatory cells. The results of this study suggest that MIF plays an important role in the pathogenesis of NEC and may serve as a target for therapeutic intervention in NEC. © 2005 Blackwell Publishing Limited.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/HISen_HK
dc.relation.ispartofHistopathologyen_HK
dc.rightsHistopathology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectIL-6en_HK
dc.subjectIL-8en_HK
dc.subjectMIFen_HK
dc.subjectNECen_HK
dc.subject.meshAcute Diseaseen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCulture Media, Conditioned - chemistryen_HK
dc.subject.meshEnterocolitis, Necrotizing - genetics - metabolism - pathologyen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshInfant, Newbornen_HK
dc.subject.meshInterleukin-6 - blooden_HK
dc.subject.meshInterleukin-8 - blooden_HK
dc.subject.meshMacrophage Migration-Inhibitory Factors - blood - genetics - metabolismen_HK
dc.subject.meshMacrophages - cytology - metabolismen_HK
dc.subject.meshRNA, Messenger - genetics - metabolismen_HK
dc.subject.meshUp-Regulation - geneticsen_HK
dc.titleUp-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0309-0167&volume=46&issue=6&spage=659&epage=667&date=2005&atitle=Up-regulation+of+macrophage+migration+inhibitory+factor+in+infants+with+acute+neonatal+necrotizing+enterocolitisen_HK
dc.identifier.emailLui, V: vchlui@hkucc.hku.hken_HK
dc.identifier.emailNicholls, J: nicholls@pathology.hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.authorityLui, V=rp00363en_HK
dc.identifier.authorityNicholls, J=rp00364en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1365-2559.2005.02159.xen_HK
dc.identifier.pmid15910597-
dc.identifier.scopuseid_2-s2.0-20644434611en_HK
dc.identifier.hkuros98968en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-20644434611&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue6en_HK
dc.identifier.spage659en_HK
dc.identifier.epage667en_HK
dc.identifier.isiWOS:000229780800007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridRen, Y=8109150500en_HK
dc.identifier.scopusauthoridLin, CL=27169299500en_HK
dc.identifier.scopusauthoridLi, Z=36064156900en_HK
dc.identifier.scopusauthoridChen, XY=8611578500en_HK
dc.identifier.scopusauthoridHuang, X=55212099100en_HK
dc.identifier.scopusauthoridLui, V=7004231344en_HK
dc.identifier.scopusauthoridNicholls, J=7201463077en_HK
dc.identifier.scopusauthoridLan, HY=7102710832en_HK
dc.identifier.scopusauthoridTam, PKH=7202539421en_HK
dc.identifier.citeulike204015-

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