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Article: Detection and evaluation of p53 intron 2 polymorphism in lung carcinomas in Hong Kong

TitleDetection and evaluation of p53 intron 2 polymorphism in lung carcinomas in Hong Kong
Authors
Issue Date1996
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 1996, v. 69 n. 2, p. 120-124 How to Cite?
AbstractA polymorphism in intron 2 of the p53 gene, which gives rise to 2 alleles, A1 and A2, was analyzed by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and direct DNA-sequencing techniques. The distribution of this allele in the peripheral blood in the Chinese population comprising 27 healthy individuals, 30 bronchiectasis patients, 34 non-small-cell lung cancer (NSCLC) patients and 27 SCLC patients was analyzed. The genotypic distributions for this marker were significantly different between the blood of healthy individuals and SCLC patients. There was no significant difference between genotypes of Caucasians and Chinese. Tumors, normal lungs and peripheral blood of 83 adenocarcinoma and 10 squamous cell carcinoma patients were also studied. There was a significant difference in the distribution of the genotypes detected in tumor tissues vs. blood of adenocarcinoma patients. The frequency of detection of the A1/A1 genotype in the tumor tissues was increased in adenocarcinoma patients as compared with the blood of adenocarcinoma patients and was decreased in the blood of SCLC patients as compared with the blood of healthy individuals. Survival rates in Hong Kong adenocarcinoma patients with the A1/A1 genotype were lower than those in patients with A1/A2 and A2/A2 genotypes up to 30 months post-operation. Point mutations were detected at the p53 intron 2 polymorphic locus in NSCLC specimens, with a mutation rate of 15.4% (8/52). All mutations were GC transversions. The significance of this instability in p53 intron 2 remains to be elucidated.
Persistent Identifierhttp://hdl.handle.net/10722/88547
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGe, Hen_HK
dc.contributor.authorLam, WKen_HK
dc.contributor.authorLee, Jen_HK
dc.contributor.authorWong, MPen_HK
dc.contributor.authorFu, KHen_HK
dc.contributor.authorYew, WWen_HK
dc.contributor.authorLung, MLen_HK
dc.date.accessioned2010-09-06T09:44:48Z-
dc.date.available2010-09-06T09:44:48Z-
dc.date.issued1996en_HK
dc.identifier.citationInternational Journal Of Cancer, 1996, v. 69 n. 2, p. 120-124en_HK
dc.identifier.issn0020-7136en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88547-
dc.description.abstractA polymorphism in intron 2 of the p53 gene, which gives rise to 2 alleles, A1 and A2, was analyzed by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and direct DNA-sequencing techniques. The distribution of this allele in the peripheral blood in the Chinese population comprising 27 healthy individuals, 30 bronchiectasis patients, 34 non-small-cell lung cancer (NSCLC) patients and 27 SCLC patients was analyzed. The genotypic distributions for this marker were significantly different between the blood of healthy individuals and SCLC patients. There was no significant difference between genotypes of Caucasians and Chinese. Tumors, normal lungs and peripheral blood of 83 adenocarcinoma and 10 squamous cell carcinoma patients were also studied. There was a significant difference in the distribution of the genotypes detected in tumor tissues vs. blood of adenocarcinoma patients. The frequency of detection of the A1/A1 genotype in the tumor tissues was increased in adenocarcinoma patients as compared with the blood of adenocarcinoma patients and was decreased in the blood of SCLC patients as compared with the blood of healthy individuals. Survival rates in Hong Kong adenocarcinoma patients with the A1/A1 genotype were lower than those in patients with A1/A2 and A2/A2 genotypes up to 30 months post-operation. Point mutations were detected at the p53 intron 2 polymorphic locus in NSCLC specimens, with a mutation rate of 15.4% (8/52). All mutations were GC transversions. The significance of this instability in p53 intron 2 remains to be elucidated.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_HK
dc.relation.ispartofInternational Journal of Canceren_HK
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.subject.meshAdenocarcinoma - geneticsen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAsian Continental Ancestry Groupen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBronchiectasis - geneticsen_HK
dc.subject.meshCarcinoma, Non-Small-Cell Lung - geneticsen_HK
dc.subject.meshCarcinoma, Small Cell - geneticsen_HK
dc.subject.meshCarcinoma, Squamous Cell - geneticsen_HK
dc.subject.meshEuropean Continental Ancestry Groupen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGenes, p53en_HK
dc.subject.meshHong Kongen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIntronsen_HK
dc.subject.meshLung Neoplasms - geneticsen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPolymorphism, Geneticen_HK
dc.subject.meshPolymorphism, Single-Stranded Conformationalen_HK
dc.subject.meshSmokingen_HK
dc.subject.meshSurvival Analysisen_HK
dc.subject.meshTumor Suppressor Protein p53 - metabolismen_HK
dc.titleDetection and evaluation of p53 intron 2 polymorphism in lung carcinomas in Hong Kongen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=69&spage=120&epage=124&date=1996&atitle=Detection+and+evaluation+of+p53+intron+2+polymorphism+in+lung+carcinomas+in+Hong+Kongen_HK
dc.identifier.emailWong, MP:mwpik@hkucc.hku.hken_HK
dc.identifier.emailLung, ML:mlilung@hku.hken_HK
dc.identifier.authorityWong, MP=rp00348en_HK
dc.identifier.authorityLung, ML=rp00300en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1097-0215(19960422)69:2<120::AID-IJC9>3.0.CO;2-2en_HK
dc.identifier.pmid8608979-
dc.identifier.scopuseid_2-s2.0-0029885181en_HK
dc.identifier.hkuros11934en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029885181&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume69en_HK
dc.identifier.issue2en_HK
dc.identifier.spage120en_HK
dc.identifier.epage124en_HK
dc.identifier.isiWOS:A1996UH13500009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGe, H=55137880800en_HK
dc.identifier.scopusauthoridLam, WK=7203021937en_HK
dc.identifier.scopusauthoridLee, J=27169550700en_HK
dc.identifier.scopusauthoridWong, MP=7403907887en_HK
dc.identifier.scopusauthoridFu, KH=7202283800en_HK
dc.identifier.scopusauthoridYew, WW=16940459600en_HK
dc.identifier.scopusauthoridLung, ML=7006411788en_HK

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