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Article: Comparative analysis of Epstein-Barr virus gene polymorphisms in nasal T/NK-cell lymphomas and normal nasal tissues: Implications on virus strain selection in malignancy

TitleComparative analysis of Epstein-Barr virus gene polymorphisms in nasal T/NK-cell lymphomas and normal nasal tissues: Implications on virus strain selection in malignancy
Authors
Issue Date1999
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 1999, v. 80 n. 3, p. 356-364 How to Cite?
AbstractWhether particular Epstein-Barr virus (EBV) strains are preferentially selected in malignant diseases remains controversial. Assessment of the importance of strain variation in the pathogenicity of EBV has been hampered principally by the lack of accurate data on the prevalence of virus variants in the normal population. To clarify this issue, a detailed comparative analysis of the EBV genomes contained in normal nasal and nasopharyngeal mucosal tissues and in nasal T/NK-cell lymphoma, which originates at these anatomic sites, was carried out by PCR amplification across the 30-bp deletion and the 33-bp repeat loci in the LMPI gene and the typespecific polymorphic loci in the EBNA2 and EBNA3C genes and by sequence analysis of the 3' C-terminal region of the LMPI gene. Whilst the majority of EBV strains in either normal or tumour tissues were type I viruses with similar numbers of LMPI repeats, a marked predominance of LMPI deletion (del-LMP) over non- deleted/wild-type LMPI (wt-LMPI) variants was observed in nasal T/NK-cell lymphoma. Although del-LMPI variants were also prevalent in the normal carriers of our population, wt-LMPI was detected at a significantly higher frequency in normal vs. tumour tissues (p = 0.036). More critically, wt-LMPI variants were found frequently in mixed infection with del-LMPI variants in the normal carriers. Sequence analysis identified 2 major del-LMPI (and several wt-LMPI) variants containing signatory nucleotide changes in relation to the prototype B95-8 sequence in both normal and neoplastic nasal tissues. Together, our data provide strong evidence for a selection mechanism for del- LMPI over the wt-LMPI variants in tumours.
Persistent Identifierhttp://hdl.handle.net/10722/88529
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChiang, AKSen_HK
dc.contributor.authorWong, KYen_HK
dc.contributor.authorLiang, ACTen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.date.accessioned2010-09-06T09:44:33Z-
dc.date.available2010-09-06T09:44:33Z-
dc.date.issued1999en_HK
dc.identifier.citationInternational Journal Of Cancer, 1999, v. 80 n. 3, p. 356-364en_HK
dc.identifier.issn0020-7136en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88529-
dc.description.abstractWhether particular Epstein-Barr virus (EBV) strains are preferentially selected in malignant diseases remains controversial. Assessment of the importance of strain variation in the pathogenicity of EBV has been hampered principally by the lack of accurate data on the prevalence of virus variants in the normal population. To clarify this issue, a detailed comparative analysis of the EBV genomes contained in normal nasal and nasopharyngeal mucosal tissues and in nasal T/NK-cell lymphoma, which originates at these anatomic sites, was carried out by PCR amplification across the 30-bp deletion and the 33-bp repeat loci in the LMPI gene and the typespecific polymorphic loci in the EBNA2 and EBNA3C genes and by sequence analysis of the 3' C-terminal region of the LMPI gene. Whilst the majority of EBV strains in either normal or tumour tissues were type I viruses with similar numbers of LMPI repeats, a marked predominance of LMPI deletion (del-LMP) over non- deleted/wild-type LMPI (wt-LMPI) variants was observed in nasal T/NK-cell lymphoma. Although del-LMPI variants were also prevalent in the normal carriers of our population, wt-LMPI was detected at a significantly higher frequency in normal vs. tumour tissues (p = 0.036). More critically, wt-LMPI variants were found frequently in mixed infection with del-LMPI variants in the normal carriers. Sequence analysis identified 2 major del-LMPI (and several wt-LMPI) variants containing signatory nucleotide changes in relation to the prototype B95-8 sequence in both normal and neoplastic nasal tissues. Together, our data provide strong evidence for a selection mechanism for del- LMPI over the wt-LMPI variants in tumours.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_HK
dc.relation.ispartofInternational Journal of Canceren_HK
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.subject.meshAntigens, Viral - geneticsen_HK
dc.subject.meshGene Deletionen_HK
dc.subject.meshGenes, Viral - geneticsen_HK
dc.subject.meshHerpesvirus 4, Human - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKiller Cells, Naturalen_HK
dc.subject.meshLymphoma, T-Cell - geneticsen_HK
dc.subject.meshNose - virologyen_HK
dc.subject.meshNose Neoplasms - genetics - virologyen_HK
dc.subject.meshPolymorphism, Genetic - geneticsen_HK
dc.subject.meshViral Matrix Proteins - geneticsen_HK
dc.titleComparative analysis of Epstein-Barr virus gene polymorphisms in nasal T/NK-cell lymphomas and normal nasal tissues: Implications on virus strain selection in malignancyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=80&spage=356&epage=364&date=1999&atitle=Comparative+analysis+of+Epstein-barr+virus+gene+polymorphisms+in+nasal+T/NK-cell+lymphomas+and+normal+nasal+tissues:+implications+on+virus+strain+selection+in+malignancyen_HK
dc.identifier.emailChiang, AKS:chiangak@hkucc.hku.hken_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authorityChiang, AKS=rp00403en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1097-0215(19990129)80:3<356::AID-IJC4>3.0.CO;2-Den_HK
dc.identifier.pmid9935174en_HK
dc.identifier.scopuseid_2-s2.0-0032933462en_HK
dc.identifier.hkuros43067en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032933462&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume80en_HK
dc.identifier.issue3en_HK
dc.identifier.spage356en_HK
dc.identifier.epage364en_HK
dc.identifier.isiWOS:000077782700004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChiang, AKS=7101623534en_HK
dc.identifier.scopusauthoridWong, KY=7404758500en_HK
dc.identifier.scopusauthoridLiang, ACT=14825292900en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK

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