File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Transcriptional activity of the promoter region of rat frizzled-related protein gene

TitleTranscriptional activity of the promoter region of rat frizzled-related protein gene
Authors
Issue Date2001
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2001, v. 286 n. 1, p. 94-100 How to Cite?
AbstractFrizzled-related protein (Frp) is a new family of secreted proteins involved in tumorigenesis and Wnt-signaling pathway. Previous study has shown that rat Frp (rFrp) gene was found to be differentially expressed in Rat 6 fibroblast cell line overexpressing p53val135 (R6#13-8). The rFrp gene was otherwise silent in normal parental Rat 6 cells. To elucidate the molecular basis of the transcriptional activation of rFrp, we have isolated and analyzed a 2-kilobase pair promoter region of the rFrp gene. Mapping of transcription initiation sites of rFrp showed the existence of multiple initiation sites. Transfection studies of serial deletion constructs in both Rat 6 and CHOK1 cell lines revealed that the region from -202 to -144 contains cis-acting elements essential for the efficient transcription of rFrp. This work provides a transcriptional regulation basis for Frp and gives insight into its implication in tumorigenesis. © 2001 Academic Press.
Persistent Identifierhttp://hdl.handle.net/10722/88520
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPing Yam, JWen_HK
dc.contributor.authorChan, KWen_HK
dc.contributor.authorWai Wong, VKen_HK
dc.contributor.authorWendy Hsiao, WLen_HK
dc.date.accessioned2010-09-06T09:44:26Z-
dc.date.available2010-09-06T09:44:26Z-
dc.date.issued2001en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2001, v. 286 n. 1, p. 94-100en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/88520-
dc.description.abstractFrizzled-related protein (Frp) is a new family of secreted proteins involved in tumorigenesis and Wnt-signaling pathway. Previous study has shown that rat Frp (rFrp) gene was found to be differentially expressed in Rat 6 fibroblast cell line overexpressing p53val135 (R6#13-8). The rFrp gene was otherwise silent in normal parental Rat 6 cells. To elucidate the molecular basis of the transcriptional activation of rFrp, we have isolated and analyzed a 2-kilobase pair promoter region of the rFrp gene. Mapping of transcription initiation sites of rFrp showed the existence of multiple initiation sites. Transfection studies of serial deletion constructs in both Rat 6 and CHOK1 cell lines revealed that the region from -202 to -144 contains cis-acting elements essential for the efficient transcription of rFrp. This work provides a transcriptional regulation basis for Frp and gives insight into its implication in tumorigenesis. © 2001 Academic Press.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCHO Cellsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshDNAen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPromoter Regions, Geneticen_HK
dc.subject.meshProteins - geneticsen_HK
dc.subject.meshRatsen_HK
dc.subject.meshTranscription, Geneticen_HK
dc.titleTranscriptional activity of the promoter region of rat frizzled-related protein geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=286&spage=94&epage=100&date=2001&atitle=Transcriptional+activity+of+the+promoter+region+of+rat+Frizzled-related+protein+geneen_HK
dc.identifier.emailPing Yam, JW:judyyam@pathology.hku.hken_HK
dc.identifier.authorityPing Yam, JW=rp00468en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/bbrc.2001.5349en_HK
dc.identifier.pmid11485313-
dc.identifier.scopuseid_2-s2.0-0034816421en_HK
dc.identifier.hkuros63383en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034816421&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume286en_HK
dc.identifier.issue1en_HK
dc.identifier.spage94en_HK
dc.identifier.epage100en_HK
dc.identifier.isiWOS:000170519100015-
dc.publisher.placeUnited Statesen_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats